Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 578,890 (T)7→8 coding (4/294 nt) borD ← DLP12 prophage; putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913578,8831.T94.4% 364.3 / 14.5 130coding (11/294 nt)borDDLP12 prophage; putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base . (4/3);  new base T (61/58);  total (68/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

TCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCA‑‑TTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATT  >  NC_000913/578736‑579025
                                                                                                                                                     |                                                                                                                                                    
tCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGATATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTTTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCA‑‑t                                                                                                                                                    <  2:1544949/149‑1 (MQ=255)
  gACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCA‑‑ttt                                                                                                                                                 >  1:809601/1‑149 (MQ=255)
  gACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCA‑‑ttt                                                                                                                                                 >  1:1859942/1‑149 (MQ=255)
  gACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCA‑‑ttt                                                                                                                                                 >  1:1705936/1‑149 (MQ=255)
     agatttCTTCTGCCCAATTCCAGAAACGAAGAATTGATGGGTGATGGTTACATTTGGTGCTACTGATGTCTGTTTGTTTTTAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGGCAGCGCATTAGCGAGTAGCA‑‑ttt‑‑‑ttt                                                                                                                                          <  1:880438/146‑1 (MQ=255)
       ttttCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCA‑‑ttt‑‑‑ttttt                                                                                                                                        >  2:884174/1‑148 (MQ=255)
        tttCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCA‑‑ttt‑‑‑tttttc                                                                                                                                       <  2:612244/149‑3 (MQ=255)
          tcttctGGCCATTTCCATCAACGAAGATATGAGGGGTGTTGGTTTCCTTTGGTGCTCCTGCTGTCTGTGTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTTATACGCAGGGCCAGCGCAGTTGCGAGTAGCA‑‑ttt‑‑‑tttttcat                                                                                                                                     <  2:1001026/149‑5 (MQ=255)
            ttctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATgg                                                                                                                                    <  1:1700148/149‑1 (MQ=255)
            ttctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATgg                                                                                                                                    >  2:1689309/1‑149 (MQ=255)
              ctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGGCTGTTTGTTTTGAACAGTAAATGTCTGTTGATCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGgtg                                                                                                                                  <  1:1468181/149‑1 (MQ=255)
                  ccAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTAt                                                                                                                              <  1:1142371/149‑1 (MQ=255)
                   cAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTAtt                                                                                                                             >  1:1379822/1‑149 (MQ=255)
                     aTTCCAGTCACGATGAAATTATGGGTGATGGTTTCCTTTGGGCCAACTGCTGTCTGTTTGTTTTGAACAGTAAATCTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGGAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTcc                                                                                                                           <  2:1503889/149‑1 (MQ=255)
                      ttCCAGAACCGAAGAAATGTGGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACCGTAAATGTCTGTTGTGCACATCCTGTAAAAAGCAGGGCCAGCGCAGTAGCGAGTAGCA‑‑TTT‑‑‑TTATTCATGGTGTTATTccc                                                                                                                          <  2:688395/149‑1 (MQ=255)
                         cAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGAt                                                                                                                       >  1:619521/1‑149 (MQ=255)
                         cAGAAACGAAGAAATGATGGGTGATGGTTTCCTTAGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGATGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGAt                                                                                                                       >  2:1573064/1‑149 (MQ=255)
                          aGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTATATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGAAGCGAGTAGCATTTT‑‑‑TTTTCATCGTGTTATTCCCGATg                                                                                                                      >  1:1734926/1‑149 (MQ=255)
                          aGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATg                                                                                                                      >  1:80677/1‑149 (MQ=255)
                          aGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATg                                                                                                                      >  1:549669/1‑149 (MQ=255)
                             aaCGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCtt                                                                                                                   >  1:1518307/1‑149 (MQ=255)
                               cGAAGAACTGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCtttt                                                                                                                 >  1:1807591/1‑149 (MQ=255)
                                gaagaaATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAATTGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCT‑‑TTTTTATTTTCATGGTGTTATTACACATGCttt                                                                                                                  >  2:993326/1‑149 (MQ=255)
                                 aagaaATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCTCATGCTGTAATAAGCTGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTTTTACCGATGCTTTTTg                                                                                                               >  1:1003467/1‑149 (MQ=255)
                                 aagaaATGATGGGTGATGGTTTCATTTGGTGCTACTGCTGTCTGTTTGATTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATAGCCGATGCTTTTTg                                                                                                               >  1:1072871/1‑149 (MQ=255)
                                  agaaATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGa                                                                                                              <  1:733831/149‑1 (MQ=255)
                                   taaaTGATGTGTGATGGTTACCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGaa                                                                                                             <  1:563658/148‑1 (MQ=255)
                                   taaaTGATGGGCGATGGTGTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGaa                                                                                                             <  2:79124/148‑1 (MQ=255)
                                         atgGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGc                                                                                                       <  2:832822/149‑1 (MQ=255)
                                             gTGAGGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGAATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGaa                                                                                                   >  2:651678/1‑149 (MQ=255)
                                              ggATGGGTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGGCTGTTGAGCACATCCTGTAATAAGCAGGGACAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAAt                                                                                                  <  1:1230669/148‑1 (MQ=255)
                                                 tGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGt                                                                                               >  1:628509/1‑149 (MQ=255)
                                                   gTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTAt                                                                                             >  2:1588872/1‑149 (MQ=255)
                                                     ttCCTTTGGGGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTAtgt                                                                                           <  1:1003969/149‑1 (MQ=255)
                                                       ccTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATgtgt                                                                                         >  2:20028/1‑149 (MQ=255)
                                                       ccTTTGGTGCTACTGCTGTCTGTTAGTTTTGAACAGTAAATGTCTGTTGAGCACATACTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATgtgt                                                                                         <  2:140720/149‑1 (MQ=255)
                                                         tttGGTGCTACTGCTGGCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAg                                                                                       <  2:1540336/149‑1 (MQ=255)
                                                           tGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGaa                                                                                     >  2:273928/1‑149 (MQ=255)
                                                             gTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGaaaa                                                                                   <  1:410117/149‑1 (MQ=255)
                                                             gTGCTACTGCTGTCTGGTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGaaaa                                                                                   <  2:354500/149‑1 (MQ=255)
                                                               gCTACTGCTGTCTGTTTGTTTTGAACAGTCACTGTCTGATGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGAATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTGGAAGTTCGCAGAATCGTATGTGTAGATAAtt                                                                                 <  2:1149181/149‑1 (MQ=255)
                                                                 tACTGCTGTCTGGTTGTTTTTAACAGTAAATGGGTGTTGAGCTCATCCTGTAATATGTAGGGCGAGAGCAGTTTCGTGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGTTTTTTGAAGTTCGCAGACTCGTATGTGTAGAAAATTaa                                                                               <  2:1026030/149‑1 (MQ=255)
                                                                  aCTTCTGTCTGTTTGTTGTGAACAGTAAATGTCTGTTGATCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCA‑‑TTT‑‑‑TTATTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTaaa                                                                              <  1:1331963/149‑1 (MQ=255)
                                                                  aCTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTaaa                                                                              >  1:48842/1‑149 (MQ=255)
                                                                              ttgtttTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAAc                                                                  <  2:1379822/149‑1 (MQ=255)
                                                                               tgtttTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCATGGCCAGCACAGTATAGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGAAGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACa                                                                 <  1:1813517/149‑1 (MQ=255)
                                                                                 ttttGAAGAGTTAATGTCTGTTGAGCACATCGTTTAATAAGCAGGGCCAGCGCAGTAGCTTGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAAt                                                               <  1:243565/149‑1 (MQ=255)
                                                                                 ttttGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAAt                                                               <  2:763432/149‑1 (MQ=255)
                                                                                 ttttGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAAt                                                               >  1:106724/1‑149 (MQ=255)
                                                                                   ttGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGa                                                             >  1:661701/1‑149 (MQ=255)
                                                                                   ttGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTACCGATGCTTTTTTAAGTTCTCAGAATCGAATGTGTAGAAAATTAAACAAACCCTAAACAATGa                                                             >  1:858969/1‑149 (MQ=255)
                                                                                       aCAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTg                                                         >  2:840593/1‑149 (MQ=255)
                                                                                         aGTAAATGTCTGTTGTGCTCATCCTGTAATAAGCAGGGCCAGCGCTGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTTTTCCCGATGCTTTTTGAAGTTGTCAGAATCGTATGTGTAGAATTTTAAACTAACCCTAAACAATGAGTTGaa                                                       <  1:1135398/149‑1 (MQ=255)
                                                                                         aGTAAATGTCTGTTGAGCACATCCTGAAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGCTGTTATTCCCGATGCATTTTGAAGTTCGAAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGaa                                                       >  1:68223/1‑149 (MQ=255)
                                                                                          gTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTAATGGTGTTATTCCCCCTGGTTTTTGAAGTTCGCATAAAAGTATGTGTATAAAATTAAACAAACCCTAAACAATGATTTTaaa                                                      >  1:844040/1‑149 (MQ=255)
                                                                                            aaaTGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAAtt                                                    <  2:1590235/149‑1 (MQ=255)
                                                                                              aTGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGGGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTc                                                  <  2:1705936/149‑1 (MQ=255)
                                                                                                gTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAATATTCat                                                >  1:459289/1‑149 (MQ=255)
                                                                                                 tCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCata                                               >  2:31135/1‑149 (MQ=255)
                                                                                                 tCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCata                                               >  2:1818087/1‑149 (MQ=255)
                                                                                                   tGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGCATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATAtt                                             >  1:298469/1‑149 (MQ=255)
                                                                                                     ttGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGt                                           >  1:573921/1‑149 (MQ=255)
                                                                                                       gAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTa                                         >  1:134011/1‑149 (MQ=255)
                                                                                                         ccacaTCCTGTAATAAGCAGGGACAGATAAGTAGAGAGTAGCATTTT‑‑‑TTTTCATTGTGTTAATACCGATGCTGTTTTAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTTCACAATGAGTTGAAATTTCATATTGTTAat                                       <  1:1222901/148‑1 (MQ=255)
                                                                                                          cacaTCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAata                                      >  1:507680/1‑149 (MQ=255)
                                                                                                          cacaTCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAata                                      >  2:1329162/1‑149 (MQ=255)
                                                                                                          cacaTCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAata                                      <  1:716074/149‑1 (MQ=255)
                                                                                                           acaTCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAatat                                     >  1:165488/1‑149 (MQ=255)
                                                                                                            caTCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAAtatt                                    >  1:10259/1‑149 (MQ=255)
                                                                                                              tCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAAtattta                                  >  1:707401/1‑149 (MQ=255)
                                                                                                                  gTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTaa                              >  2:1315796/1‑149 (MQ=255)
                                                                                                                   taataaGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAAt                             >  2:1530686/1‑149 (MQ=255)
                                                                                                                   taataaGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAAt                             >  1:409649/1‑149 (MQ=255)
                                                                                                                   taataaGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAAt                             >  1:1113683/1‑149 (MQ=255)
                                                                                                                   taataaGCAGGGCCAGCGCAGTAGCGAGTAGCAGTTT‑‑‑TTTTCATGGTGTGATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGGTGAAATTTCATATTGTTAATATTTATTAAt                             >  2:1068099/1‑149 (MQ=255)
                                                                                                                    aataaGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGTAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATg                            <  1:1573064/149‑1 (MQ=255)
                                                                                                                      taaGCATGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTa                          <  1:1689309/149‑1 (MQ=255)
                                                                                                                      taaGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTa                          >  1:801796/1‑149 (MQ=255)
                                                                                                                       aaccAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTAt                         <  2:125240/146‑1 (MQ=255)
                                                                                                                       aaGCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAACCCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTAt                         >  1:824299/1‑149 (MQ=255)
                                                                                                                        aGCAGGTCCAGCGAAGTAGCGAGTAGCAA‑‑TAT‑‑‑TTTTCATGGGGTTATTCCCGCAGCTTTATCAAGTTGGCAGAATAGTAAGTGAAGAAAAATAAAAAAAACCTAAACAAAGTGTTGAAATTTCATATTGTTAATATTTATTAATCTAtt                        >  2:1864224/1‑148 (MQ=255)
                                                                                                                         gCAGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCACGATGCTTTTTTAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACACTAAAAAATGAGTTGAAAATTCATATTGTTAATAATTATTAATGTATGc                       >  2:353145/1‑149 (MQ=255)
                                                                                                                           aGGGCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCa                     <  1:884174/149‑1 (MQ=255)
                                                                                                                             ggCCAGCGCAGTAGCGAGTAGCAGTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAAGCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAgg                   >  2:829551/1‑149 (MQ=255)
                                                                                                                              gCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTATAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGt                  >  1:1858489/1‑149 (MQ=255)
                                                                                                                              gCCAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATATATTAATGTATGCCAGGt                  >  1:1237697/1‑149 (MQ=255)
                                                                                                                               ccAGCGCAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTg                 <  2:461821/149‑1 (MQ=255)
                                                                                                                                cAGCGCTGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGc                >  1:242340/1‑149 (MQ=255)
                                                                                                                                   cgcatgaGCGAGTATCA‑‑TATT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGAt             <  2:515829/143‑1 (MQ=255)
                                                                                                                                   cgcAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGAt             <  2:981811/149‑1 (MQ=255)
                                                                                                                                    gcAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATg            <  2:636926/149‑1 (MQ=255)
                                                                                                                                    gcAGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATg            <  2:549669/149‑1 (MQ=255)
                                                                                                                                      aGTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTAATCCCGTTGCTTTTTGAAGTGCGCAGAATCGTATGTGACGAAAATTAAACAACCCCTAAACACTGAGGTGAAATTTCATATTGTTAATATTTATTAATGTATGACAGGTGCGATGaa          <  1:1640399/149‑1 (MQ=255)
                                                                                                                                       gTAGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAAt         <  1:31135/149‑1 (MQ=255)
                                                                                                                                        tAGCGAGTAGCATTTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAAt         <  2:614430/149‑1 (MQ=255)
                                                                                                                                         aGCGAGTTGCATTTT‑‑‑TTTTCATGGTTTTATTCACGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCg       <  2:858969/149‑1 (MQ=255)
                                                                                                                                         aGCGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCg       <  2:1518307/149‑1 (MQ=255)
                                                                                                                                           cGAGTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTc     <  2:707401/149‑1 (MQ=255)
                                                                                                                                           cGAGTAGCAGTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTGTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTc     <  2:122362/149‑1 (MQ=255)
                                                                                                                                              gTAGCATTTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTCAtt  <  2:661701/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCA‑‑TTT‑‑‑TTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATT  >  NC_000913/578736‑579025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: