Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,182,9230CT56.4% 35.1 / 131.9 113P560L (CCA→CTA) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base T (32/30);  total (58/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.47e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 116 total aligned reads displayed.

CTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATC  >  NC_000913/4182775‑4183071
                                                                                                                                                    |                                                                                                                                                    
cTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCCCTTCGGTCGCGTATGTcc                                                                                                                                                      >  2:451825/1‑149 (MQ=255)
       aGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGATCGTGCAGGCTTCGAAGTTCGAGACGTACACGCGACTCACTTCGGTCGCGTATGtcgaatcgaa                                                                                                                                               >  2:89421/1‑149 (MQ=255)
       aGTTTATGGACCAGAACAACCCGCTGTCTGAGATTAAGCACAAACGTCGTATCTCAGCACTCGGCCCAGGCTGTATGCCCCTTGAACGTGCAGGCTTCGAAGTTCGAGACGTACAACCGACTAACTACGGTCGAGTTTGTCTAATCGaa                                                                                                                                               >  2:356412/1‑149 (MQ=255)
          ttATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCGTCGAAGTTAGAGACGTACACCCGCATCACTACGGTCGCGTATGTCCAATCGAAAcc                                                                                                                                            >  2:138435/1‑149 (MQ=255)
           tATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAccc                                                                                                                                           <  1:1705219/149‑1 (MQ=255)
           tATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAccc                                                                                                                                           <  1:191697/149‑1 (MQ=255)
                cccAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACCCCCGCCTCACTACGGTCGCGTATGGCCATTCGCAACCCCTGaa                                                                                                                                      >  2:1025754/2‑149 (MQ=255)
                aCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGaa                                                                                                                                      <  1:1234126/149‑1 (MQ=255)
                aCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGaa                                                                                                                                      <  1:295685/149‑1 (MQ=255)
                aCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTACGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGaa                                                                                                                                      >  1:886378/1‑149 (MQ=255)
                  cAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAgg                                                                                                                                    >  1:1409987/1‑149 (MQ=255)
                        aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGaa                                                                                                                              >  1:1682829/1‑149 (MQ=255)
                          cacGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCAATCGGCCCACGCGGTCTGACCCGTGATCGTGCAGGATTCGAAGTACGTGACGTACAACCGACTCACTAAGGTCGCGTATGTCTAATCGAAACCCATTAAGGTCCGAGCa                                                                                                                            >  1:1188931/3‑149 (MQ=255)
                           ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACAt                                                                                                                           <  1:787796/149‑1 (MQ=255)
                            cGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTCCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATc                                                                                                                          <  2:692068/149‑1 (MQ=255)
                             gCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGTTCGCGTATTTCTAATCGAAACCCCTGAAGGTCCGAACAACg                                                                                                                         >  2:805698/1‑149 (MQ=255)
                             gCTGACTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGGGAACGTGCAGGCTGCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                                                         <  1:1184348/149‑1 (MQ=255)
                               tggcTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGGCCAGGCGGTCTGACGCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGt                                                                                                                       <  2:771366/146‑1 (MQ=255)
                                   tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTCCACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACACCTGAAGGTCCGAACATCGGTCTGa                                                                                                                   >  1:1010899/1‑149 (MQ=255)
                                   tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGa                                                                                                                   >  1:22064/1‑149 (MQ=255)
                                   tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCAGAACATCGGTCTGa                                                                                                                   >  1:1608363/1‑149 (MQ=255)
                                   tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTgg                                                                                                                   >  1:723581/1‑148 (MQ=255)
                                     agaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATc                                                                                                                 >  1:212742/1‑149 (MQ=255)
                                       aTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGATACGACTGTAGGTCCGACCAGCTGTCTGATCaa                                                                                                               >  2:1275939/1‑149 (MQ=255)
                                         tACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAAct                                                                                                             <  1:1621162/149‑1 (MQ=255)
                                            gCACAAACGTCGTATCTCTGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctc                                                                                                          <  2:810184/149‑1 (MQ=255)
                                            gCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctc                                                                                                          >  2:865877/1‑149 (MQ=255)
                                             cacaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctct                                                                                                         >  1:898445/1‑149 (MQ=255)
                                              acaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGATCGAGACGTACACCCGCCTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGATCATCGGTCTGATCAACTCTCTg                                                                                                        >  1:1082528/1‑149 (MQ=255)
                                              acaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATAGGTCTGATAAACTCTCTg                                                                                                        >  2:1509659/1‑149 (MQ=255)
                                              acaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACACACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg                                                                                                        >  2:1204281/1‑149 (MQ=255)
                                                 aaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTcc                                                                                                     <  2:519119/149‑1 (MQ=255)
                                                   cgtcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGAGCAGGCTTCGAAGTGCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt                                                                                                   <  1:946885/149‑1 (MQ=255)
                                                    gtcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCAGACTCACGACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTGTGCTCAACTCTCTGTCCgtg                                                                                                  >  2:1496285/1‑149 (MQ=255)
                                                     tcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGATCGTGCAGGCTTAGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGACACCCCTGAAGGTCCGAACATCGGGCTGATCACCTCTCTGTCCgtgt                                                                                                 >  2:1024358/1‑149 (MQ=255)
                                                       gtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCTGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                                                                               <  1:1025754/149‑1 (MQ=255)
                                                       gtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                                                                               <  1:1204281/149‑1 (MQ=255)
                                                        tATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg                                                                                              <  2:1669591/149‑1 (MQ=255)
                                                            tcCGCACTCGGCCCAGGCGGTCTGACCCGTTAACGGGCAGGCGTCGAAGTTCGAGACGTACACCCGTCTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTACGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGcaca                                                                                          <  2:1010899/149‑1 (MQ=255)
                                                            tcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGcaca                                                                                          <  2:1608363/149‑1 (MQ=255)
                                                            tcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGcaca                                                                                          <  2:22064/149‑1 (MQ=255)
                                                             ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAg                                                                                         >  1:1105243/1‑149 (MQ=255)
                                                             ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGGCTGATCAACTCTCTGTCCGTGTACGCACAg                                                                                         >  1:1374549/1‑149 (MQ=255)
                                                              cGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGCACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGa                                                                                        >  1:854823/1‑149 (MQ=255)
                                                              cGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGCAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGa                                                                                        >  1:1697473/1‑149 (MQ=255)
                                                              cGCACTCGGCCCACGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGGTAGAGCTGTACACCCGACTCACTAGGGTCTCGTATTTCCATTCGAAAGCCCTGAAGGTCAGAAAATCTGTCTGATGAACTCTCTGTCCGTGTACGCACAGa                                                                                        <  2:1462971/149‑1 (MQ=255)
                                                               gCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTGCGTACACGGACAGag                                                                                       <  1:634458/149‑2 (MQ=255)
                                                                 aCTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCCCGTAAGTCTAATCCAAACCCCTGAAGGTCCAAACATCGGTCTGCTCCACTCTCTTTCCGTGGACGCACAGCCTa                                                                                     >  2:455490/1‑149 (MQ=255)
                                                                  cTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTaa                                                                                    <  2:212742/149‑1 (MQ=255)
                                                                   tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAc                                                                                       <  2:1436230/145‑1 (MQ=255)
                                                                   tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAc                                                                                       >  1:1436230/1‑145 (MQ=255)
                                                                   tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAAc                                                                                   <  2:767509/149‑1 (MQ=255)
                                                                   tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAAc                                                                                   <  1:560143/149‑1 (MQ=255)
                                                                      gCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGaa                                                                                >  1:537788/1‑149 (MQ=255)
                                                                      gCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGaa                                                                                <  2:549181/149‑1 (MQ=255)
                                                                       cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCCCTACGGTCGCGTATGTCGAATCGCAACCCCGGCAGGTCCGAACAGAGGTCTGGTCAACTCTCTGTCCTTGTAAGCACCGACTAACGCAt                                                                               >  2:1306965/1‑149 (MQ=255)
                                                                       cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAAt                                                                               <  1:138435/149‑1 (MQ=255)
                                                                         cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTAAGGACGCGTATGTCTAATCTAAACACCTGAAGTTCCGAACGTCGGTCTGATCAAATCTCATTCAGTGAACGCACATACTAACCaaaaa                                                                             >  2:1135908/1‑146 (MQ=255)
                                                                         cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACAACCGACTCACAAAGGTCGCGTATGTCAAATAGAAACACCAGAAGGTAAGAACATCGGTCAGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc                                                                             >  1:1123715/1‑149 (MQ=255)
                                                                           ggCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg                                                                           <  2:873346/149‑1 (MQ=255)
                                                                                tCTGACCCGTGAACGTGCAGGCTGCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTcc                                                                      <  1:1520347/149‑1 (MQ=255)
                                                                                  tGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt                                                                    <  2:1682829/149‑1 (MQ=255)
                                                                                         ggAACGTGCAGGCTACGAAGACCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc                                                             <  2:723581/148‑1 (MQ=255)
                                                                                             cGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTa                                                         <  1:1716479/149‑1 (MQ=255)
                                                                                             cGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTATAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTa                                                         <  1:1440916/149‑1 (MQ=255)
                                                                                               tGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATc                                                       <  2:1166809/149‑1 (MQ=255)
                                                                                               tGCAGGCTTCGAAGTACGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATc                                                       <  2:886378/149‑1 (MQ=255)
                                                                                                gCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCg                                                      >  2:391407/1‑149 (MQ=255)
                                                                                                          acggtCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCGCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCTTATCGTAAAGTGAcc                                            <  1:1024358/145‑1 (MQ=255)
                                                                                                          aaGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCTATCGAAACCCCTGAAGGTCCGAACATCGGGCTGATCAACTCTCTGTCCGGGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAcc                                            >  1:829717/1‑149 (MQ=255)
                                                                                                             ttCGAGACGTACACCCGACTCACAACGGTCGCGTAAGTCTAATCGCAACCCCAGAAAGTCCGCACATCGGTCTGATCACCTCTCTGTCCCTGAACGCACAGACTAACCAAAACGGCTTCCTTGAGACTCCCTAACGTAAAGTGACCGAc                                         >  2:1364134/1‑149 (MQ=255)
                                                                                                                  gaCGTACACCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGgtgt                                    <  2:1082528/149‑1 (MQ=255)
                                                                                                                     gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGtgttgt                                 >  1:604324/1‑149 (MQ=255)
                                                                                                                        cacCCGACTCACTACGGTCGCGTATGTTCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAAc                              >  1:1286745/1‑149 (MQ=255)
                                                                                                                         acCCGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCCCAGACTAACGAATACGGCTTCCTTGAGCCTCCGGATCGGAAAGTGACCGACGgtgtgggatcg                             >  2:1179599/1‑142 (MQ=255)
                                                                                                                           ccGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGa                           <  1:682685/149‑1 (MQ=255)
                                                                                                                           ccGACTCACTACGGTCGCGTATGTCTAATAGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGa                           <  1:1509659/149‑1 (MQ=255)
                                                                                                                           ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGCATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGa                           >  2:189750/1‑149 (MQ=255)
                                                                                                                           ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTTACTgc                           >  2:169452/1‑148 (MQ=255)
                                                                                                                           ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGa                           >  1:471172/1‑149 (MQ=255)
                                                                                                                           ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGATTCCTTGAGACTCCGTTTCGGATAGTGACCGACGGGGTGGTAACTGa                           >  1:1153833/1‑149 (MQ=255)
                                                                                                                           ccGAATCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGa                           <  1:345735/149‑1 (MQ=255)
                                                                                                                            cGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGAc                          >  1:927218/1‑149 (MQ=255)
                                                                                                                            cGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGAc                          >  1:994756/1‑149 (MQ=255)
                                                                                                                            cGACTCACTACGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACACACAGACTAACGAATACGGCTTCCTTGAGCCTCCGTTTCGTAAAGTGACCGACGGTGTTGTAACTGAc                          >  1:1186867/1‑149 (MQ=255)
                                                                                                                                  aCTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACTGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAAtt                    >  2:1538807/1‑149 (MQ=255)
                                                                                                                                    tACGGTCGCGTATGTCCAATCGAAACCCCTGAATGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCa                  <  1:154877/149‑1 (MQ=255)
                                                                                                                                    tACGGTCGCGTATGTCCAATCGAAACCCCAGAAGGTACGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACACCGTATCGTAAAGTGACCGACGTTTTTGTAACTGACGAAATTCa                  >  2:1445438/1‑149 (MQ=255)
                                                                                                                                     aCGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCAc                 <  1:1204589/149‑1 (MQ=255)
                                                                                                                                      cGGTCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGCACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTTAAGTGACCGACGGTGTTGTAACGGACGAAATTCAcg                >  2:1053027/1‑148 (MQ=255)
                                                                                                                                      cGGTCGCGTATGTCTAATAGAAACCCCAGAAGGTCCGAACAAATGTATGATAAACTCTCATTCAGTGTACGAACAGACTAACGAATACGGCATCCTTGATACTACCTATACTAAAGTGCCCCTCGGTGTTGTAAATGAAGAAAtgaacc                >  2:910079/1‑144 (MQ=255)
                                                                                                                                       ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCAGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTa               >  2:1335239/1‑149 (MQ=255)
                                                                                                                                       ggTCGCGTATGTCCAAGCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTCGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTa               <  1:451825/149‑1 (MQ=255)
                                                                                                                                         tCGCGTATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTAcc             >  1:173292/1‑149 (MQ=255)
                                                                                                                                            cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGt          >  1:660092/1‑149 (MQ=255)
                                                                                                                                              tATGTCTAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCt        <  2:1374549/149‑1 (MQ=255)
                                                                                                                                              tATGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCt        <  1:391407/149‑1 (MQ=255)
                                                                                                                                              tATGTCTAATCGAAACCCCTGAAGGGCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCt        <  2:1105243/149‑1 (MQ=255)
                                                                                                                                               aTGTCTAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTg       <  2:898445/149‑1 (MQ=255)
                                                                                                                                                    cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATc  >  1:486666/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATC  >  NC_000913/4182775‑4183071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑