Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 4,183,235 G→A G664D (GGT→GAT)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi4,183,2350GA100.0% 713.8 / NA 192G664D (GGT→GAT) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (98/94);  total (98/94)

Only 100 of 192 total aligned reads displayed.

TTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCC  >  NC_000913_3_pae_pgi/4183088‑4183380
                                                                                                                                                   |                                                                                                                                                 
 tATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGc                                                                                                                                                <  1:1214722/150‑1 (MQ=255)
      cccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTccc                                                                                                                                           >  2:1716998/1‑150 (MQ=255)
       ccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCt                                                                                                                                          <  1:2362517/150‑1 (MQ=255)
       ccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCt                                                                                                                                          >  1:1151794/1‑150 (MQ=255)
           gCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATc                                                                                                                                      >  1:861745/1‑150 (MQ=255)
                   cAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCt                                                                                                                              <  3:533275/150‑1 (MQ=255)
                    aaCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTg                                                                                                                             <  2:2319521/150‑1 (MQ=255)
                    aaCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTg                                                                                                                             <  2:1175020/150‑1 (MQ=255)
                          gATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAacac                                                                                                                       <  4:432779/150‑1 (MQ=255)
                               agaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTc                                                                                                                                >  4:868115/1‑136 (MQ=255)
                                gaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGAc                                                                                                                 <  2:1151794/150‑1 (MQ=255)
                                 aagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACg                                                                                                                >  4:22574/1‑150 (MQ=255)
                                       aCTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGa                                                                                                                  <  1:1182238/142‑1 (MQ=255)
                                         ttCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGt                                                                                                        >  4:190854/1‑150 (MQ=255)
                                                 agaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGAt                                                                                                <  1:248871/150‑1 (MQ=255)
                                                   aCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATgg                                                                                              >  1:1084090/1‑150 (MQ=255)
                                                     cTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGt                                                                                            >  2:205001/1‑150 (MQ=255)
                                                      tGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGATCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTg                                                                                           <  3:395972/150‑1 (MQ=255)
                                                      tGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTg                                                                                           <  1:2165378/150‑1 (MQ=255)
                                                      tGGTAACTTGCCGTAGCAAAGGCGAATACCGATTGTTTAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTg                                                                                           >  2:1247474/1‑150 (MQ=255)
                                                       ggTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGa                                                                                                                                        >  2:1038964/1‑104 (MQ=255)
                                                       ggTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGa                                                                                                                                        <  1:1038964/104‑1 (MQ=255)
                                                           aCTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACATATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCt                                                                                                                              >  1:514723/1‑110 (MQ=255)
                                                             ttGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAc                                                                                                           >  2:1202736/1‑127 (MQ=255)
                                                             ttGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAt                                                                                    >  4:58680/1‑150 (MQ=255)
                                                              tGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATg                                                                                   <  1:343162/150‑1 (MQ=255)
                                                                ccGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTc                                                                                                                                >  1:2450913/1‑103 (MQ=255)
                                                                ccGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGaa                                                                                                                          >  2:1133867/1‑109 (MQ=255)
                                                                ccGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTg                                                                                                       >  1:148067/1‑128 (MQ=255)
                                                                ccGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGAGCCCGTTc                                                                                                                                <  2:2450913/103‑1 (MQ=255)
                                                                    aGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGt                                                                             >  4:611706/1‑150 (MQ=255)
                                                                      cAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGt                                                                                            <  2:2092305/133‑1 (MQ=255)
                                                                      cAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGt                                                                                            >  1:2092305/1‑133 (MQ=255)
                                                                        aaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAgg                                                                         >  1:2059884/1‑150 (MQ=255)
                                                                           gCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAt                                                                                    >  3:1087235/1‑136 (MQ=255)
                                                                           gCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAt                                                                                    <  4:1087235/136‑1 (MQ=255)
                                                                           gCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCg                                                                      >  3:701990/1‑150 (MQ=255)
                                                                            cGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGt                                                                     >  2:1184236/1‑150 (MQ=255)
                                                                               ctCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCACCGTCAGGCCGTTcc                                                                  <  4:1044424/149‑1 (MQ=255)
                                                                               aTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTcc                                                                  >  4:798515/1‑150 (MQ=255)
                                                                                  cAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGcc                                                                                                              <  2:2243475/103‑1 (MQ=255)
                                                                                  cAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGcc                                                                                                              >  1:2243475/1‑103 (MQ=255)
                                                                                   aGCTTGTTCAGCCGCGACCAGGTTGACTATATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCGTGATCCCGTTCCTGGAACACGATGAAGCCAACCGTGCATTGATGGGTGCGAGCATGCAACGTCAGGCCGTTACGAcc                                                              >  2:1906398/1‑149 (MQ=255)
                                                                                   aGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCaa                                                                                                            <  2:291174/104‑1 (MQ=255)
                                                                                   aGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCaa                                                                                                            >  1:291174/1‑104 (MQ=255)
                                                                                   aGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAct                                                              >  2:1574761/1‑150 (MQ=255)
                                                                                     cTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGActct                                                            >  1:1424493/1‑150 (MQ=255)
                                                                                        gTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcg                                                         >  4:1263195/1‑150 (MQ=255)
                                                                                           cAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgcgc                                                      >  1:1263123/1‑150 (MQ=255)
                                                                                            aGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTcc                                                                                                                                            <  1:1777119/63‑1 (MQ=255)
                                                                                            aGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTcc                                                                                                                                            >  2:1777119/1‑63 (MQ=255)
                                                                                              ccGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGa                                                   >  4:422006/1‑150 (MQ=255)
                                                                                              ccGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGa                                                   <  2:996902/150‑1 (MQ=255)
                                                                                                  gACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCt                                                                                                                                          >  1:394154/1‑59 (MQ=255)
                                                                                                  gACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCt                                                                                                                                          <  2:394154/59‑1 (MQ=255)
                                                                                                     cAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATg                                                                                                                   >  2:436805/1‑79 (MQ=255)
                                                                                                     cAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAAGCAACGTCAGGCCGCTCCGACTCTATGCGCTGATAAGCCg                                            >  3:519854/1‑150 (MQ=255)
                                                                                                         ttGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtgg                                        <  1:205001/150‑1 (MQ=255)
                                                                                                              tACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGt                                                                                                                                  >  1:2146942/1‑55 (MQ=255)
                                                                                                              tACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttggt                                   >  2:1392752/1‑150 (MQ=255)
                                                                                                                   ggACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAacac                                                                                                                       >  2:2143916/1‑61 (MQ=255)
                                                                                                                   ggACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGa                                                                >  2:403717/1‑116 (MQ=255)
                                                                                                                   ggACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                  >  1:644067/1‑146 (MQ=255)
                                                                                                                   ggACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                                  <  2:644067/146‑1 (MQ=255)
                                                                                                                    gACGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGAt                                                                                                <  3:422455/83‑1 (MQ=255)
                                                                                                                     aCGTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTc                                                                   <  1:1905371/111‑1 (MQ=255)
                                                                                                                       gTATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCTTTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg                                    <  3:353631/140‑1 (MQ=255)
                                                                                                                        tATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGAt                                                                                                <  4:960879/79‑1 (MQ=255)
                                                                                                                        tATCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGAt                                                                                                >  3:960879/1‑79 (MQ=255)
                                                                                                                         aTCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGa                        <  3:58680/150‑1 (MQ=255)
                                                                                                                          tCCACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc                                                                        <  1:432156/101‑1 (MQ=255)
                                                                                                                            cACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGt                                                                             <  1:1542663/94‑1 (MQ=255)
                                                                                                                            cACCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGt                                                                             >  2:1542663/1‑94 (MQ=255)
                                                                                                                             aCCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTg                                                                                                                             >  3:1235671/1‑45 (MQ=255)
                                                                                                                             aCCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTg                                                                                                  >  3:223555/1‑72 (MQ=255)
                                                                                                                             aCCCAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGc                                                                                          <  4:467895/80‑1 (MQ=255)
                                                                                                                             aCCAAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGa                                                                <  1:1748774/106‑1 (MQ=255)
                                                                                                                             aCCAAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGa                                                                >  2:1748774/1‑106 (MQ=255)
                                                                                                                               ccAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACg                                                                                                                >  4:878333/1‑56 (MQ=255)
                                                                                                                               ccAGCAGGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACg                                                                                                                <  3:878333/56‑1 (MQ=255)
                                                                                                                                 agcagGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGa                                                   <  4:382827/115‑1 (MQ=255)
                                                                                                                                 agcagGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTg                <  3:798515/150‑1 (MQ=255)
                                                                                                                                 agcagGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTg                >  1:1620750/1‑150 (MQ=255)
                                                                                                                                   cagGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGtt              <  2:861506/150‑1 (MQ=255)
                                                                                                                                   cagGTGGTATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGtt              >  4:199605/1‑150 (MQ=255)
                                                                                                                                      gtggtATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAct                                                              <  4:1329517/99‑1 (MQ=255)
                                                                                                                                      gtggtATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAct                                                              >  3:1329517/1‑99 (MQ=255)
                                                                                                                                      gtggtATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAAc                      >  2:1816297/1‑139 (MQ=255)
                                                                                                                                      gtggtATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAAc                      <  1:1816297/139‑1 (MQ=255)
                                                                                                                                       tggtATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCg          <  1:802868/150‑1 (MQ=255)
                                                                                                                                       tggtATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCg          >  3:1426088/1‑150 (MQ=255)
                                                                                                                                        ggtATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCGACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggtt                                      <  2:626442/121‑1 (MQ=255)
                                                                                                                                        ggtATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCGACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggtt                                      >  1:626442/1‑121 (MQ=255)
                                                                                                                                        ggtATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt                                       >  2:561130/1‑120 (MQ=255)
                                                                                                                                         gtATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGt                                                                                            <  4:1187677/66‑1 (MQ=255)
                                                                                                                                         gtATCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGtt        <  1:2136054/150‑1 (MQ=255)
                                                                                                                                           aTCCGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCg                                            <  2:1453165/112‑1 (MQ=255)
                                                                                                                                              cGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTc   >  2:2111145/1‑150 (MQ=255)
                                                                                                                                              cGTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTc   <  2:2059860/150‑1 (MQ=255)
                                                                                                                                               gTCGATGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTcc  >  2:1983099/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCC  >  NC_000913_3_pae_pgi/4183088‑4183380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: