Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 3,106,112 G→T P133P (CCG→CCT nupG → nucleoside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,106,1120GT92.0% 415.9 / 21.9 138P133P (CCG→CCTnupGnucleoside transporter
Reads supporting (aligned to +/- strand):  ref base G (5/6);  new base T (69/57);  total (75/63)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 138 total aligned reads displayed.

ATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGTTTACCCTGACTCTGCCGCATAT  >  NC_000913_3_pae_pgi/3105966‑3106258
                                                                                                                                                  |                                                                                                                                                  
aTGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCACTCCa                                                                                                                                                 >  4:859858/1‑150 (MQ=255)
  ggcggcACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAAt                                                                                                                                               >  2:1295922/1‑150 (MQ=255)
   gcggcACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATc                                                                                                                                              >  2:1302394/1‑150 (MQ=255)
        acaGGTCACGACACCGGAAGCGTTGTTCCTTGTGGTAGTAATTAACTCGCTTGCTTAGATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATGTCGTTACTGACTTCCCTCCAATCCGTAt                                                                                                                                         <  1:1178347/150‑1 (MQ=255)
                    acCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCAt                                                                                                                             >  1:572327/1‑150 (MQ=255)
                      cGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCg                                                                                                                           >  2:547912/1‑150 (MQ=255)
                       ggAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCgg                                                                                                                          >  4:834210/1‑150 (MQ=255)
                        gAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGc                                                                                                                         >  3:18891/1‑150 (MQ=255)
                          aGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCtt                                                                                                                       <  3:548732/150‑1 (MQ=255)
                            cGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTa                                                                                                                     <  1:1255051/150‑1 (MQ=255)
                              aTGTTCCTTGTGATATTGATTCACTCGTTTGCTTCTATGCCACCTCTTGCGCATTTTCATTTTATTTTTTTATATTGACCGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATc                                                                                                                   <  1:1009441/150‑1 (MQ=255)
                               tGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCa                                                                                                                  >  1:149997/1‑150 (MQ=255)
                                        tgATATTGATTGTCTCGTTTGTTTATATGTCAGCGCTTGGGTTATTCAACCCTATCTCTTACTATCTCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAAtgt                                                                                                         <  1:822136/150‑1 (MQ=255)
                                         gATAATGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCaa                                                                                                            <  4:848678/146‑1 (MQ=255)
                                          atgttGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGAACTgtgg                                                                                                       <  2:491627/147‑1 (MQ=255)
                                          atatTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTggg                                                                                                                                    <  1:1149166/121‑1 (MQ=255)
                                          atatTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTggg                                                                                                                                    >  2:1149166/1‑121 (MQ=255)
                                          atatTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATgtgg                                                                                                       <  1:120563/150‑1 (MQ=255)
                                              tGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATgtgggtgg                                                                                                   <  4:701872/150‑1 (MQ=255)
                                                 ttAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGa                                                                                                <  3:629665/150‑1 (MQ=255)
                                                  tAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAg                                                                                               >  1:344176/1‑150 (MQ=255)
                                                   aaCTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGc                                                                                              <  4:711247/150‑1 (MQ=255)
                                                        gTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTc                                                                                         >  3:475436/1‑150 (MQ=255)
                                                            gCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGc                                                                                     <  2:1150677/150‑1 (MQ=255)
                                                             cTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCtt                                                                                                                       >  1:1593304/1‑115 (MQ=255)
                                                             cTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCtt                                                                                                                       <  2:1593304/115‑1 (MQ=255)
                                                              ttATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCtt                                                                                   >  1:1526876/1‑150 (MQ=255)
                                                              tcatatGCCAACGCTTGGGTTAATCAACACCACCTCTTACTAGCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCAGGGCANTGTGGGTGGTGAGCCTGTCTGGCtt                                                                                   <  1:1030166/148‑1 (MQ=255)
                                                                  atGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATgtgg                                                                                                       >  1:745020/1‑126 (MQ=255)
                                                                  atGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATgtgg                                                                                                       <  2:745020/126‑1 (MQ=255)
                                                                  atGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGaa                                                                               <  4:723288/150‑1 (MQ=255)
                                                                    gCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAAtt                                                                             >  2:1639664/1‑150 (MQ=255)
                                                                     cgaaCGCTTGGGTTAATCAACACCATCTTTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCACAAATACGTATCAGGGGCACCATCGGCTTTATCATGGCTATGTGGGGGGTGAGCCTGTTTGGGTTCGAATTa                                                                            >  1:1190096/3‑150 (MQ=255)
                                                                     ccAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATAGGATTTATCATGGCAATGTGGGTGGTGAGCCAGTCTGGCTTTGAATTa                                                                            >  4:263377/1‑150 (MQ=255)
                                                                      cAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCAt                                                                                                                 >  1:664565/1‑112 (MQ=255)
                                                                      cAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTaa                                                                           <  3:363501/150‑1 (MQ=255)
                                                                      cAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTaa                                                                           <  4:749925/150‑1 (MQ=255)
                                                                       aaCGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAg                                                                          >  2:579160/1‑150 (MQ=255)
                                                                       aaCGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTAAGGACTTCCCTGCGATCCATAGCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAg                                                                          >  1:589086/1‑150 (MQ=255)
                                                                        aCGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGc                                                                         <  2:1637119/150‑1 (MQ=255)
                                                                          gCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCAc                                                                                                                                <  4:131185/93‑1 (MQ=255)
                                                                                gTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATgtgggt                                                                                                     >  2:997792/1‑114 (MQ=255)
                                                                                gTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATgtgggt                                                                                                     <  1:997792/114‑1 (MQ=255)
                                                                                gTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTaa                                                                           >  1:852769/1‑140 (MQ=255)
                                                                                gTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTaa                                                                           <  2:852769/140‑1 (MQ=255)
                                                                                gTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACAGACTTCCCTCCAATCCGTATCTggg                                                                                                                                    <  4:1026057/83‑1 (MQ=255)
                                                                                gTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACAGACTTCCCTCCAATCCGTATCTggg                                                                                                                                    >  3:1026057/1‑83 (MQ=255)
                                                                                   aaTCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCt                                                              <  4:909611/150‑1 (MQ=255)
                                                                                    aTCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTg                                                             >  3:758053/1‑150 (MQ=255)
                                                                                     tCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTgg                                                                                                                                     >  2:724697/1‑77 (MQ=255)
                                                                                     tCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTgg                                                                                                                                     <  1:724697/77‑1 (MQ=255)
                                                                                     tCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTTGGGAACCAACGGATCCATCACTGTAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGt                                                            >  1:68229/1‑150 (MQ=255)
                                                                                     tCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGt                                                            >  3:691691/1‑150 (MQ=255)
                                                                                     tCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGt                                                            >  3:884114/1‑150 (MQ=255)
                                                                                       aaCACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCt                                                                                        <  2:1469015/120‑1 (MQ=255)
                                                                                       aaCACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCt                                                                                        >  1:1469015/1‑120 (MQ=255)
                                                                                        acacCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATgtgg                                                                                                       <  3:148084/104‑1 (MQ=255)
                                                                                        acacCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATgtgg                                                                                                       >  4:148084/1‑104 (MQ=255)
                                                                                        acacCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGc                                                                  >  3:961637/1‑141 (MQ=255)
                                                                                        acacCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGtata                                                         <  3:834210/150‑1 (MQ=255)
                                                                                         cacCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTatat                                                        >  1:1609710/1‑150 (MQ=255)
                                                                                         cacCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGc                                                                                     >  3:257660/1‑121 (MQ=255)
                                                                                         cacCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGc                                                                                     <  4:257660/121‑1 (MQ=255)
                                                                                         cacCAACTCTTACGATCGCCTGCAAAATGCTGGCATGGATATCGTTACTGACTTCCCTCCACTCCGTATCTGTGGCACCATCGGCTTTATTATCGCAATGTGGATGGTGAGCCTGTCTGGCTTCGAATTAATTTACATGCAGCTGTatat                                                        <  2:1190096/150‑1 (MQ=255)
                                                                                            cATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTgg                                                     >  2:391357/1‑150 (MQ=255)
                                                                                            cATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTgg                                                     >  2:391649/1‑150 (MQ=255)
                                                                                               ctctTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTaa                                                                           >  4:716447/1‑125 (MQ=255)
                                                                                               ctctTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTaa                                                                           <  3:716447/125‑1 (MQ=255)
                                                                                               ctctTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGgcgc                                                  <  2:344176/150‑1 (MQ=255)
                                                                                                      tATCGCCTGCAAAATTCCGGGATGGATATCGTTACTGACTTCCCTCCAATACGTATCAGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGACTTCGAATTAAGCCACATGTAGCTGTATATCGGCGCAGCACtt                                           >  2:1453392/1‑150 (MQ=255)
                                                                                                       aTCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACttt                                          <  1:770017/150‑1 (MQ=255)
                                                                                                           ccTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGc                                      >  4:353608/1‑150 (MQ=255)
                                                                                                           ccTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGc                                      >  3:469748/1‑150 (MQ=255)
                                                                                                           ccTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATATGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGc                                      >  2:1030202/1‑150 (MQ=255)
                                                                                                             tGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCa                                    >  2:288577/1‑150 (MQ=255)
                                                                                                             tGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCa                                    >  4:141123/1‑150 (MQ=255)
                                                                                                             tGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATATGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCa                                    >  1:1489373/1‑150 (MQ=255)
                                                                                                              gCAAAATGCCGGGATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCAt                                   >  3:819148/1‑150 (MQ=255)
                                                                                                                  aaTGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTg                               <  1:986083/150‑1 (MQ=255)
                                                                                                                        gggATGGATATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTg                         <  2:1609710/150‑1 (MQ=255)
                                                                                                                         ggatggatATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGt                        <  1:288577/150‑1 (MQ=255)
                                                                                                                          gatggatATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGtt                       <  1:936684/150‑1 (MQ=255)
                                                                                                                          gatggatATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGGGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGtt                       <  2:1303251/150‑1 (MQ=255)
                                                                                                                           ttggatATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGttt                      <  3:335537/149‑1 (MQ=255)
                                                                                                                             ggatATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGTTTAc                    <  1:579160/150‑1 (MQ=255)
                                                                                                                              gatATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTaa                                                                           >  2:1679482/1‑94 (MQ=255)
                                                                                                                              gatATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTaa                                                                           <  1:1679482/94‑1 (MQ=255)
                                                                                                                              gatATCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGTTTAcc                   <  1:1693776/150‑1 (MQ=255)
                                                                                                                                tatCGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGtt                            <  2:117751/139‑1 (MQ=255)
                                                                                                                                   cGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATgtgg                                                                                                       <  2:1399071/61‑1 (MQ=255)
                                                                                                                                   cGTTACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATgtgg                                                                                                       >  1:1399071/1‑61 (MQ=255)
                                                                                                                                     ttACTGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGTTTACCCTGActc            >  4:952929/1‑150 (MQ=255)
                                                                                                                                         tGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTg                                                      >  3:1052345/1‑104 (MQ=255)
                                                                                                                                         tGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGTTTACCCTGACTCTGcc        >  1:343632/1‑150 (MQ=255)
                                                                                                                                         tGACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGTTTACCCTGACTCTGcc        <  4:475436/150‑1 (MQ=255)
                                                                                                                                          gACTTCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGTTTACCCTGACTCTGCCg       >  2:493298/1‑150 (MQ=255)
                                                                                                                                             ttCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCt                                                              <  3:721139/92‑1 (MQ=255)
                                                                                                                                             ttCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGTTTACCCTGACTCTGCCGCat    >  4:55102/1‑150 (MQ=255)
                                                                                                                                             ttCCCTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGTTTACCCTGACTCTGCCGCat    >  2:574232/1‑150 (MQ=255)
                                                                                                                                               cccTCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGTTTACCCTGACTCTGCCGCatat  >  2:445165/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATGCAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGTTTACCCTGACTCTGCCGCATAT  >  NC_000913_3_pae_pgi/3105966‑3106258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: