Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,166,727 G→C E215Q (GAA→CAA)  ndh → respiratory NADH dehydrogenase 2/cupric reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,166,7270GC99.4% 560.2 / ‑6.2 155E215Q (GAA→CAA) ndhrespiratory NADH dehydrogenase 2/cupric reductase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (72/82);  total (73/82)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

CGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCG  >  NC_000913_3_pae_pgi/1166584‑1166871
                                                                                                                                               |                                                                                                                                                
cgAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGAAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCgg                                                                                                                                            <  2:814976/150‑1 (MQ=255)
     ggCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGga                                                                                                                                          >  1:1836993/1‑147 (MQ=255)
       cAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCTGCTGAATTGCACAACGCGGTCACGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACg                                                                                                                                     >  2:1213383/1‑150 (MQ=255)
       cAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACg                                                                                                                                     >  1:278891/1‑150 (MQ=255)
          aGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAt                                                                                                                                  <  1:982493/150‑1 (MQ=255)
              aaCATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTg                                                                                                                              >  1:475482/1‑150 (MQ=255)
                cATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGcc                                                                                                                            <  1:130255/150‑1 (MQ=255)
                 aTTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCt                                                                                                                           >  4:1063084/1‑150 (MQ=255)
                 aTTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCt                                                                                                                           <  4:842626/150‑1 (MQ=255)
                 aTTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCt                                                                                                                           >  1:1609172/1‑150 (MQ=255)
                      gATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGtt                                                                                                                      >  2:700243/1‑150 (MQ=255)
                        ttGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTAc                                                                                                                    >  2:1153656/1‑150 (MQ=255)
                                 gcggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTa                                                                                                           >  1:1890203/1‑150 (MQ=255)
                                   ggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTAtc                                                                                                         >  2:75987/1‑150 (MQ=255)
                                   ggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTAtc                                                                                                         >  2:168734/1‑150 (MQ=255)
                                    gcgcGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGCCCAACGAAGCCCTGAACGTAACGCTGGTACCAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTAtct                                                                                                        <  2:636785/150‑1 (MQ=255)
                                     cgcgACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCGGGTACAAGCGGGAGAACGTATTNTGCCTGCGTTACCGCCACGTAtctc                                                                                                       <  3:707179/150‑1 (MQ=255)
                                      gcgACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTAcc                                                                                                                   >  1:1784871/1‑137 (MQ=255)
                                      gcgACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTAcc                                                                                                                   <  2:1784871/137‑1 (MQ=255)
                                      gcgACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATctct                                                                                                      <  1:457228/150‑1 (MQ=255)
                                              tgtAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGc                                                                                              <  2:1511514/150‑1 (MQ=255)
                                              ggtAGAACTCTCCGCTGAATTGCACCCCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGACCGTAACGCTGGTACAAGCGGGCGACCGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGc                                                                                              <  4:653580/149‑1 (MQ=255)
                                                tAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGccc                                                                                            >  3:967577/1‑150 (MQ=255)
                                                 aGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCca                                                                                           >  3:885517/1‑150 (MQ=255)
                                                  gAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCcac                                                                                          >  2:110277/1‑150 (MQ=255)
                                                   aaCTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctgc                                                                                                    >  3:593923/1‑139 (MQ=255)
                                                   aaCTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCcaca                                                                                         <  3:464890/150‑1 (MQ=255)
                                                        tcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGc                                                                                                                                              <  3:1126744/92‑1 (MQ=255)
                                                        tcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGc                                                                                                                                              >  4:1126744/1‑92 (MQ=255)
                                                         ccGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGc                                                                                   <  1:1079947/150‑1 (MQ=255)
                                                           ggttaaTTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCAACAACGAGCTa                                                                                 >  2:1864141/5‑150 (MQ=255)
                                                            cTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTaa                                                                                <  2:653653/150‑1 (MQ=255)
                                                             tGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAAc                                                                               <  4:750641/150‑1 (MQ=255)
                                                             tGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAAc                                                                               <  3:836829/150‑1 (MQ=255)
                                                                aTTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGaa                                                                            <  2:1254075/150‑1 (MQ=255)
                                                                 ttGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGaaa                                                                           >  2:1305941/1‑150 (MQ=255)
                                                                    cacaCCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACtt                                                                        >  2:456349/1‑150 (MQ=255)
                                                                     acaACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTg                                                                       <  2:796815/150‑1 (MQ=255)
                                                                      caACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTgg                                                                      <  1:1153656/150‑1 (MQ=255)
                                                                      caACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTgg                                                                      >  4:902192/1‑150 (MQ=255)
                                                                      caACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTgg                                                                      <  1:110277/150‑1 (MQ=255)
                                                                      caACGCGGTCAAGCAACTGCACAGCTACGATTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAATCTTgg                                                                      <  2:1387008/150‑1 (MQ=255)
                                                                       aaCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTGACGAAACTTGGc                                                                     <  3:241357/150‑1 (MQ=255)
                                                                       aaCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGc                                                                     >  4:915814/1‑150 (MQ=255)
                                                                        aCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCg                                                                    <  4:453317/150‑1 (MQ=255)
                                                                             gTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTcgc                                                               >  1:720087/1‑150 (MQ=255)
                                                                                 aGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGc                                                           >  2:137834/1‑150 (MQ=255)
                                                                                  gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCt                                                          <  4:410220/150‑1 (MQ=255)
                                                                                    aaCTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGa                                                        >  4:445531/1‑150 (MQ=255)
                                                                                    aaCTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAACCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAtttt                                                                                                                               >  2:1935140/1‑79 (MQ=255)
                                                                                     aCTGCACAGCTACGGTTACAAAGGCCTGACCAACGAACCACTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGAc                                                       <  1:1004466/150‑1 (MQ=255)
                                                                                          acaGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGaaa                                                                           <  2:188291/125‑1 (MQ=255)
                                                                                          acaGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCaaa                                                  <  1:672852/150‑1 (MQ=255)
                                                                                           caGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAAc                                                 <  1:1428941/150‑1 (MQ=255)
                                                                                            aGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAAcc                                                >  4:690281/1‑150 (MQ=255)
                                                                                             gCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGc                                                           >  3:1047256/1‑138 (MQ=255)
                                                                                             gCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGc                                                           <  4:1047256/138‑1 (MQ=255)
                                                                                                 cGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGt                                           <  1:1322791/150‑1 (MQ=255)
                                                                                                     tACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCAcc                                       >  1:421561/1‑150 (MQ=255)
                                                                                                      aCAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTAt                                                                                                          >  4:1208068/1‑82 (MQ=255)
                                                                                                       cAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTaa                                                                                >  4:662348/1‑107 (MQ=255)
                                                                                                        aaaGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGt                                    >  2:1054463/1‑150 (MQ=255)
                                                                                                        aaaGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGACTGCGTTAACGCCACGTATCTCTGATGCCGAATTCAGCGGCGTATTGAAGCGAGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGt                                    >  1:1771181/1‑150 (MQ=255)
                                                                                                         aaGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTg                                   <  2:1634661/150‑1 (MQ=255)
                                                                                                         aaGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCAACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTg                                   >  1:1436513/1‑150 (MQ=255)
                                                                                                          aGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGc                                  >  4:538289/1‑150 (MQ=255)
                                                                                                           ggCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCt                                 <  3:538289/150‑1 (MQ=255)
                                                                                                            gCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCtg                                >  3:7570/1‑150 (MQ=255)
                                                                                                             ccTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGt                                                             >  1:858212/1‑120 (MQ=255)
                                                                                                              cTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCtgat                              <  3:732107/150‑1 (MQ=255)
                                                                                                               tGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTAc                                                                                                                    >  2:471750/1‑63 (MQ=255)
                                                                                                               tGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTAc                                                                                                                    <  1:471750/63‑1 (MQ=255)
                                                                                                                   cAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCcac                                                                                          <  4:831458/85‑1 (MQ=255)
                                                                                                                   cAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCcac                                                                                          >  3:831458/1‑85 (MQ=255)
                                                                                                                    aaCGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggc                        >  2:1908396/1‑150 (MQ=255)
                                                                                                                       gAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggcggc                     <  3:1148115/150‑1 (MQ=255)
                                                                                                                       gAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggcggc                     <  1:1825814/150‑1 (MQ=255)
                                                                                                                        aaGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGcc                    <  3:915814/150‑1 (MQ=255)
                                                                                                                        aaGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGcc                    >  1:1921991/1‑150 (MQ=255)
                                                                                                                         aGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCt                   <  1:953903/150‑1 (MQ=255)
                                                                                                                         aGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCt                   <  2:1105661/150‑1 (MQ=255)
                                                                                                                          gCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTg                  <  4:38897/150‑1 (MQ=255)
                                                                                                                             cTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGcac               >  1:1949023/1‑150 (MQ=255)
                                                                                                                             cTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGcac               >  3:789192/1‑150 (MQ=255)
                                                                                                                              tGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGcaca              >  1:1520766/1‑150 (MQ=255)
                                                                                                                              tGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGcaca              >  2:356283/1‑150 (MQ=255)
                                                                                                                               gAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACaa                                                                                        <  3:191241/75‑1 (MQ=255)
                                                                                                                               gAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACaa                                                                                        >  4:191241/1‑75 (MQ=255)
                                                                                                                               gAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACaa                                                                                        >  4:191253/1‑75 (MQ=255)
                                                                                                                               gAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACaa                                                                                        <  3:191253/75‑1 (MQ=255)
                                                                                                                                 aCGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCCTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTa           <  1:1213383/150‑1 (MQ=255)
                                                                                                                                  cGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTaa          <  1:1054463/150‑1 (MQ=255)
                                                                                                                                  cGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTaa          <  2:1798874/150‑1 (MQ=255)
                                                                                                                                   gTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTaaa         <  1:1904798/150‑1 (MQ=255)
                                                                                                                                      aCGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGAt      <  3:1060855/150‑1 (MQ=255)
                                                                                                                                      aCGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGAt      <  4:952935/150‑1 (MQ=255)
                                                                                                                                        gCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATgg    >  3:410941/1‑150 (MQ=255)
                                                                                                                                        gCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATgg    >  2:951679/1‑150 (MQ=255)
                                                                                                                                          tGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTaa                                                                                <  1:654863/72‑1 (MQ=255)
                                                                                                                                          tGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCg  >  1:1661800/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCG  >  NC_000913_3_pae_pgi/1166584‑1166871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: