Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 3,316,818 C→G A237P (GCG→CCG)  nusA ← transcription termination/antitermination L factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,316,8180CG99.2% 448.6 / ‑3.7 128A237P (GCG→CCG) nusAtranscription termination/antitermination L factor
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (78/49);  total (78/50)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

Only 100 of 128 total aligned reads displayed.

CACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCC  >  NC_000913_3_pae_pgi/3316675‑3316963
                                                                                                                                               |                                                                                                                                                 
 aCGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTc                                                                                                                                            >  1:443299/1‑150 (MQ=255)
  cGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCg                                                                                                                                           >  4:433784/1‑150 (MQ=255)
     aCTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCAc                                                                                                                                        >  3:684365/1‑150 (MQ=255)
         cgcCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACgaga                                                                                                                                    >  2:1094182/1‑150 (MQ=255)
                  atcatcCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGGTTATCGGTGGTTTTCACCGGGAGCTTCGCACGAGAACCCCGATc                                                                                                                           >  2:473357/1‑150 (MQ=255)
                      tcCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGa                                                                                                                       >  1:431954/1‑150 (MQ=255)
                       cccACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAg                                                                                                                      <  4:115755/150‑1 (MQ=255)
                        ccACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGc                                                                                                                     <  2:830581/150‑1 (MQ=255)
                        ccACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGc                                                                                                                     >  4:719731/1‑150 (MQ=255)
                            aGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCgct                                                                                                                 >  1:473310/1‑150 (MQ=255)
                              gACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCgctgc                                                                                                               >  3:718045/1‑150 (MQ=255)
                                 gATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCttt                                                                                                            <  4:629608/150‑1 (MQ=255)
                                 gATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCAAGAGAACCCGGATCGCGAGCCGCTGCttt                                                                                                            >  3:683483/1‑150 (MQ=255)
                                  atatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTa                                                                                                           >  1:714082/1‑150 (MQ=255)
                                   tatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTaa                                                                                                          <  3:433784/150‑1 (MQ=255)
                                   tatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTaa                                                                                                          >  2:478620/1‑150 (MQ=255)
                                    atCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAAt                                                                                                         <  3:122567/150‑1 (MQ=255)
                                      cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAAttt                                                                                                       >  4:102009/1‑150 (MQ=255)
                                       gATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTc                                                                                                      >  2:164652/1‑150 (MQ=255)
                                        aTACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCa                                                                                                     >  2:1168983/1‑150 (MQ=255)
                                        aTACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCa                                                                                                     >  3:119963/1‑150 (MQ=255)
                                          aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAAt                                                                                                   >  2:94050/1‑150 (MQ=255)
                                          aCGCTCGCCACCCAGTCCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAAt                                                                                                   <  1:1109821/150‑1 (MQ=255)
                                           cGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATc                                                                                                  >  3:17426/1‑150 (MQ=255)
                                            gataaccACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCa                                                                                                 >  2:1043382/6‑150 (MQ=255)
                                            gCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTGTATCGTTGGTTTTCACCGGGATTTTCGCACTAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCa                                                                                                 >  4:294078/1‑150 (MQ=255)
                                             cTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCAc                                                                                                <  3:452047/150‑1 (MQ=255)
                                                 ccACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAAt                                                                                                         >  3:34107/1‑137 (MQ=255)
                                                 ccACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAAt                                                                                                         <  4:34107/137‑1 (MQ=255)
                                                  cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCActtctt                                                                                           >  3:38321/1‑150 (MQ=255)
                                                     ccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACAGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGc                                                                                        <  4:583977/150‑1 (MQ=255)
                                                            aGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTc                                                                                 <  3:190761/150‑1 (MQ=255)
                                                               aGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTgg                                                                              <  1:1104516/150‑1 (MQ=255)
                                                                    ccGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGCTCATCGTTGGTTTTCACCGGGGTTTTCGCACGAGGCCCCGGATCGCGAGCCGCTGCTTTAATTCCAATCACTTCTTCGCCGATTTCTGGCACtt                                                                         >  1:727124/1‑150 (MQ=255)
                                                                     cGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACtt                                                                         <  1:717810/149‑1 (MQ=255)
                                                                     cGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACtt                                                                         >  2:717810/1‑149 (MQ=255)
                                                                      gCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGATTATCGTTGGTTTTCACCGGGAATTTCGCACGAGAACCCGGATCGCAAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCa                                                                       >  3:168983/1‑150 (MQ=255)
                                                                       ccTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCaa                                                                      <  2:1186471/150‑1 (MQ=255)
                                                                       ccTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCaa                                                                      >  3:211851/1‑150 (MQ=255)
                                                                        cTGAACGCGCGCGCCGCGTCTACCTCCGCACGCCCCTCCCGTGTCGATCCGTTTGTCGTTGGTTTTCACCGGTATTTTCGCGCGTGAACCCGTATCGCGAGCCGCTGCTTTAATTTCCATCCCTTCTTCGCCCATTTCTGGCACCTCCAt                                                                     <  4:168983/150‑1 (MQ=255)
                                                                        cTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAAt                                                                     >  3:236731/1‑150 (MQ=255)
                                                                         tGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATa                                                                    <  2:443299/150‑1 (MQ=255)
                                                                          gAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATAc                                                                   >  3:399692/1‑150 (MQ=255)
                                                                          gAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATAc                                                                   >  3:399736/1‑150 (MQ=255)
                                                                           aaCACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACg                                                                  >  2:1089743/1‑150 (MQ=255)
                                                                               cgcgcgCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAAc                                                              <  2:840427/150‑1 (MQ=255)
                                                                               cgcgcgCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAAc                                                              >  4:2190/1‑150 (MQ=255)
                                                                                gcgcgcCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCaa                                                                                                    <  2:195637/111‑1 (MQ=255)
                                                                                gcgcgcCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCaa                                                                                                    >  1:195637/1‑111 (MQ=255)
                                                                                    gcCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTc                                                         <  3:743683/150‑1 (MQ=255)
                                                                                       aCGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGAt                                                      <  4:441681/150‑1 (MQ=255)
                                                                                       aCGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGAt                                                      <  3:2190/150‑1 (MQ=255)
                                                                                         gCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCa                                                    <  2:431954/150‑1 (MQ=255)
                                                                                           gtACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGc                                                  >  1:509265/2‑150 (MQ=255)
                                                                                           aTACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGc                                                  <  1:94050/150‑1 (MQ=255)
                                                                                               cTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCAttt                                              >  4:199155/1‑150 (MQ=255)
                                                                                               cTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCAttt                                              >  3:272539/1‑150 (MQ=255)
                                                                                                 aCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAAt                                                                     <  1:1163392/125‑1 (MQ=255)
                                                                                                 aCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAAt                                                                     >  2:1163392/1‑125 (MQ=255)
                                                                                                 aCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTcc                                            >  2:618192/1‑150 (MQ=255)
                                                                                                   gCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCgg                                          <  3:339365/150‑1 (MQ=255)
                                                                                                   gCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCgg                                          >  2:1085537/1‑150 (MQ=255)
                                                                                                     aaGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTaa                                                                                                          <  3:534977/84‑1 (MQ=255)
                                                                                                     aaGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTaa                                                                                                          >  4:534977/1‑84 (MQ=255)
                                                                                                       gCACCTACCGGATCGATACGTTTGTCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTg                                      <  2:714082/150‑1 (MQ=255)
                                                                                                       gCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAATAGTTCGATCAGCATTTCCGGCTTg                                      <  1:1168983/150‑1 (MQ=255)
                                                                                                         aCCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCTCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGTAACAGTTCGATCAGCATTTCCGGCTTGGa                                    >  2:428466/1‑150 (MQ=255)
                                                                                                          ccTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGaa                                   <  2:342050/150‑1 (MQ=255)
                                                                                                           cTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAAc                                  >  2:861661/1‑150 (MQ=255)
                                                                                                            tACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCaa                                                                      >  2:311664/1‑113 (MQ=255)
                                                                                                            tACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCaa                                                                      <  1:311664/113‑1 (MQ=255)
                                                                                                             aCCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGa                                <  4:17426/150‑1 (MQ=255)
                                                                                                             aCCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGa                                <  4:203036/150‑1 (MQ=255)
                                                                                                              ccGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAg                               >  3:41980/1‑150 (MQ=255)
                                                                                                                ggATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTg                             >  2:325606/1‑150 (MQ=255)
                                                                                                                 gATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCCGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGa                            >  1:526864/1‑150 (MQ=255)
                                                                                                                  aTCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGAc                           <  2:1129888/150‑1 (MQ=255)
                                                                                                                     gATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGaa                        <  4:211851/150‑1 (MQ=255)
                                                                                                                       tACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCt                                                                                <  2:961257/92‑1 (MQ=255)
                                                                                                                       tACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCt                                                                                >  1:961257/1‑92 (MQ=255)
                                                                                                                         cGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGt                    <  1:164652/150‑1 (MQ=255)
                                                                                                                          gTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGa                         <  1:778956/144‑1 (MQ=255)
                                                                                                                          gTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGtt                   <  1:1089743/150‑1 (MQ=255)
                                                                                                                          gTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGtt                   >  3:22002/1‑150 (MQ=255)
                                                                                                                          gTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATCTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGtt                   >  3:611861/1‑150 (MQ=255)
                                                                                                                          gTTTATCGTTGGCTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGtt                   >  4:365708/1‑150 (MQ=255)
                                                                                                                           tttATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTg                  >  3:748175/1‑150 (MQ=255)
                                                                                                                            ttATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTgc                 <  4:38321/150‑1 (MQ=255)
                                                                                                                             tATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTgcg                >  2:835702/1‑150 (MQ=255)
                                                                                                                               tCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTgcgcg              >  1:959939/1‑150 (MQ=255)
                                                                                                                                 gTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGcc            >  1:324395/1‑150 (MQ=255)
                                                                                                                                  ttGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGc                                                  >  1:408254/1‑111 (MQ=255)
                                                                                                                                  ttGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCa           >  2:161571/1‑150 (MQ=255)
                                                                                                                                       ttttACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTGCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATCTCCGGCTTGGAACGAGTGAGGAATAGTTGCGCGCCACgcgc      >  1:1148889/1‑150 (MQ=255)
                                                                                                                                       tttCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACa                                                             >  2:332419/1‑95 (MQ=255)
                                                                                                                                       tttCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACa                                                             <  1:332419/95‑1 (MQ=255)
                                                                                                                                         tCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGa                                <  2:650061/122‑1 (MQ=255)
                                                                                                                                         tCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGa                                >  1:650061/1‑122 (MQ=255)
                                                                                                                                          cACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTGCCGGCTTGCAACGAGTGACGAACAGTTGCGCGCCACGCGCTTc   <  4:152871/150‑1 (MQ=255)
                                                                                                                                           aCCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTcc  >  2:728207/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCC  >  NC_000913_3_pae_pgi/3316675‑3316963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: