Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,731,621 G→T M845I (ATG→ATT lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,731,6210GT99.1% 414.7 / NA 116M845I (ATG→ATTlhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (59/56);  total (59/57)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

ATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTAC  >  NC_000913_3_pae_pgi/1731476‑1731766
                                                                                                                                                 |                                                                                                                                                 
aTCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTc                                                                                                                                               >  4:3823/1‑150 (MQ=255)
aTCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTc                                                                                                                                               <  1:163931/150‑1 (MQ=255)
 tCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATtcgtcg                                                                                                                                              >  2:702630/1‑150 (MQ=255)
  cGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGc                                                                                                                                             >  1:1036179/1‑150 (MQ=255)
  cGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGc                                                                                                                                             <  1:151428/150‑1 (MQ=255)
       cccGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGaa                                                                                                                                        <  2:912726/150‑1 (MQ=255)
       cccGCTTTGGCAACAACGGCTGCGCGCCAGACAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGaa                                                                                                                                        >  2:638930/1‑150 (MQ=255)
            tttGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTg                                                                                                                                   <  1:48865/150‑1 (MQ=255)
             ttGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTgg                                                                                                                                  <  1:518632/150‑1 (MQ=255)
              tGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGc                                                                                                                                 <  3:637424/150‑1 (MQ=255)
              tGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGc                                                                                                                                 <  3:182747/150‑1 (MQ=255)
               ggCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCg                                                                                                                                <  4:698397/150‑1 (MQ=255)
                   acaacGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAAt                                                                                                                            >  1:874087/1‑150 (MQ=255)
                   acaacGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAAt                                                                                                                            >  1:71417/1‑150 (MQ=255)
                     aacGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTc                                                                                                                          <  3:182075/150‑1 (MQ=255)
                        ggCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                       >  1:276629/1‑150 (MQ=255)
                             cgcgcCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATcc                                                                                                                  <  2:6481/150‑1 (MQ=255)
                                gcCAGTCAGTCGCTGGAAAGCGCTCAGGGATATCCGGATTTTCCAGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGGTTATGATCTTCCCACACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCgct                                                                                                               >  2:746949/1‑148 (MQ=255)
                                  cagtcagtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGt                                                                                                             <  1:638930/150‑1 (MQ=255)
                                      cagtTGCTGGAAATCGCTCAGGGATATCAAGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACTGTGGCGAAATTCAAATATCCGATGTAACg                                                                                                         <  1:1131934/150‑1 (MQ=255)
                                            cTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACt                                                                                                   >  2:191208/1‑150 (MQ=255)
                                              ggAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAc                                                                                                 >  2:78326/1‑150 (MQ=255)
                                               gAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACg                                                                                                >  2:1171282/1‑150 (MQ=255)
                                                 aaTCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGcc                                                                                              >  4:457000/1‑150 (MQ=255)
                                                  aTCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGccc                                                                                             >  1:263725/1‑150 (MQ=255)
                                                      cTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGc                                                                                         >  2:1063085/1‑150 (MQ=255)
                                                       tCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGcc                                                                                        >  4:507426/1‑150 (MQ=255)
                                                         aGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCtt                                                                                      <  3:645033/150‑1 (MQ=255)
                                                                tCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                       <  2:956873/110‑1 (MQ=255)
                                                                tCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                       >  1:956873/1‑110 (MQ=255)
                                                                 ccGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCcaca                                                                              >  2:897102/1‑150 (MQ=255)
                                                                 ccGGATTTGCCGGTCATTCTCGAAACACCACGCGAATGTCTGCAAGATGCTTAGGCTCTTCCCGCACCGGAACGTTTGATTCGTCGCCCGTAAGGTGGCGAAATACCAGTAACCGCTGTAACGACCACTACGCCCCCGCCTTTCGCcaca                                                                              >  4:189708/1‑150 (MQ=255)
                                                                  cGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACaa                                                                             <  2:392129/150‑1 (MQ=255)
                                                                  cGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACaa                                                                             >  4:292445/1‑150 (MQ=255)
                                                                   ggATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAg                                                                            >  1:445021/1‑150 (MQ=255)
                                                                     aTTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGtt                                                                          <  3:83645/150‑1 (MQ=255)
                                                                       tttCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTa                                                                        <  2:71417/150‑1 (MQ=255)
                                                                         tCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCg                                                                                   <  1:240394/137‑1 (MQ=255)
                                                                         tCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCg                                                                                   >  2:240394/1‑137 (MQ=255)
                                                                         tCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTAtt                                                                      <  1:305580/150‑1 (MQ=255)
                                                                          ccGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTg                                                                     <  1:1089761/150‑1 (MQ=255)
                                                                            ggTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGtt                                                                   >  2:482678/1‑150 (MQ=255)
                                                                             gTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTc                                                                  >  3:14940/1‑150 (MQ=255)
                                                                              tCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCg                                                                 <  1:188494/150‑1 (MQ=255)
                                                                               cATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCgg                                                                <  1:702630/150‑1 (MQ=255)
                                                                                aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGc                                                               >  3:122155/1‑150 (MQ=255)
                                                                                aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGc                                                               >  2:435074/1‑150 (MQ=255)
                                                                                aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGc                                                               >  1:1058238/1‑150 (MQ=255)
                                                                                 ttCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGc                                                               >  1:310414/1‑149 (MQ=255)
                                                                                 ttCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGc                                                               <  2:310414/149‑1 (MQ=255)
                                                                                 ttCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCt                                                              >  2:936051/1‑150 (MQ=255)
                                                                                 ttCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCt                                                              <  2:292178/150‑1 (MQ=255)
                                                                                  tctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTTGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTa                                                             >  4:375123/1‑150 (MQ=255)
                                                                                   ctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTAt                                                            <  3:292445/150‑1 (MQ=255)
                                                                                    tcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCCCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATg                                                           >  1:362291/1‑150 (MQ=255)
                                                                                          cccTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCgg                                                     <  1:1124322/150‑1 (MQ=255)
                                                                                             tACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAAt                                                  >  3:528807/1‑150 (MQ=255)
                                                                                               cgcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAc                                                                                                 >  4:695679/1‑101 (MQ=255)
                                                                                               cgcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAc                                                                                                 <  3:695679/101‑1 (MQ=255)
                                                                                                gcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTa                                               >  1:841328/1‑150 (MQ=255)
                                                                                                gcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCAAAAGTCTAATGTTCGGCTATGTCGCGGAATTTa                                               >  3:735057/1‑150 (MQ=255)
                                                                                                    aTGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTa                                           >  3:329934/1‑150 (MQ=255)
                                                                                                    aTGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTa                                           <  2:1058238/150‑1 (MQ=255)
                                                                                                      gTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTc                                                                  >  1:602364/1‑125 (MQ=255)
                                                                                                       tCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGt                                            >  1:1066366/1‑146 (MQ=255)
                                                                                                       tCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGt                                            <  2:1066366/146‑1 (MQ=255)
                                                                                                       tCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCa                                        <  1:78326/150‑1 (MQ=255)
                                                                                                       tCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCa                                        <  2:1036179/150‑1 (MQ=255)
                                                                                                         tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCaga                                      >  1:621659/1‑150 (MQ=255)
                                                                                                         tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCaga                                      <  1:542317/150‑1 (MQ=255)
                                                                                                         tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCaga                                      <  3:239495/150‑1 (MQ=255)
                                                                                                         tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCaga                                      >  1:149792/1‑150 (MQ=255)
                                                                                                             aGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTc                                                                                    >  3:518623/1‑100 (MQ=255)
                                                                                                             aGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGa                                  >  4:634689/1‑150 (MQ=255)
                                                                                                                  tttATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTcgc                                                       <  2:1215778/124‑1 (MQ=255)
                                                                                                                  tttATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTcgc                                                       >  1:1215778/1‑124 (MQ=255)
                                                                                                                   ttATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGcccc                            <  3:546766/150‑1 (MQ=255)
                                                                                                                    tATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCt                                                              <  2:158632/115‑1 (MQ=255)
                                                                                                                    tATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCt                                                              >  1:158632/1‑115 (MQ=255)
                                                                                                                      tGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCt                         <  2:276629/150‑1 (MQ=255)
                                                                                                                      tGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCt                         >  1:1130159/1‑150 (MQ=255)
                                                                                                                       gATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTg                        >  2:1136624/1‑150 (MQ=255)
                                                                                                                       gATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTg                        <  1:1081005/150‑1 (MQ=255)
                                                                                                                        aTCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTAt                                              <  2:1081690/127‑1 (MQ=255)
                                                                                                                        aTCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTAt                                              >  1:1081690/1‑127 (MQ=255)
                                                                                                                          cTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCa                     >  4:703059/1‑150 (MQ=255)
                                                                                                                          cTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCa                     <  1:936051/150‑1 (MQ=255)
                                                                                                                            tCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTCCGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCaga                   <  3:189708/150‑1 (MQ=255)
                                                                                                                              ccGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAgc                 >  4:633143/1‑150 (MQ=255)
                                                                                                                               cGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAgcg                >  4:31559/1‑150 (MQ=255)
                                                                                                                               cGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAgcg                >  1:1032654/1‑150 (MQ=255)
                                                                                                                                   cTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCgg            <  3:760146/150‑1 (MQ=255)
                                                                                                                                    tGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCgg                                                                <  1:1041717/97‑1 (MQ=255)
                                                                                                                                     ggAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGc          >  1:310121/1‑150 (MQ=255)
                                                                                                                                      gAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCa         >  1:495500/1‑150 (MQ=255)
                                                                                                                                          gTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCg     <  4:14940/150‑1 (MQ=255)
                                                                                                                                          gTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCg     <  3:634689/150‑1 (MQ=255)
                                                                                                                                             tGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTAc  <  2:1130159/150‑1 (MQ=255)
                                                                                                                                              gATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGt                                                          >  2:1006544/1‑93 (MQ=255)
                                                                                                                                              gATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGt                                                          <  1:1006544/93‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTAC  >  NC_000913_3_pae_pgi/1731476‑1731766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: