mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | SvNSRound4_26_25_S2431_L007_R1_001.good.fq | 201,907 | 25,494,160 | 100.0% | 126.3 bases | 150 bases | 93.1% |
| errors | SvNSRound4_26_25_S2431_L007_R2_001.good.fq | 201,907 | 25,494,160 | 100.0% | 126.3 bases | 150 bases | 92.5% |
| errors | SvNSRound4_26_25_S2431_L008_R1_001.good.fq | 124,203 | 15,789,894 | 100.0% | 127.1 bases | 150 bases | 92.6% |
| errors | SvNSRound4_26_25_S2431_L008_R2_001.good.fq | 124,203 | 15,789,894 | 100.0% | 127.1 bases | 150 bases | 91.7% |
| total | 652,220 | 82,568,108 | 100.0% | 126.6 bases | 150 bases | 92.6% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913_3_pae_pgi | 4,640,912 | 16.5 | 1.9 | 100.0% | Escherichia coli str. K-12 substr. MG1655, P. aerophilum pgi gene, complete genome. |
| total | 4,640,912 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 36925 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 140 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.009 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913_3_pae_pgi | 0.93920 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 18:00:55 19 Dec 2020 | 18:01:31 19 Dec 2020 | 36 seconds |
| Read alignment to reference genome | 18:01:32 19 Dec 2020 | 18:06:19 19 Dec 2020 | 4 minutes 47 seconds |
| Preprocessing alignments for candidate junction identification | 18:06:19 19 Dec 2020 | 18:06:47 19 Dec 2020 | 28 seconds |
| Preliminary analysis of coverage distribution | 18:06:47 19 Dec 2020 | 18:07:41 19 Dec 2020 | 54 seconds |
| Identifying junction candidates | 18:07:41 19 Dec 2020 | 18:08:35 19 Dec 2020 | 54 seconds |
| Re-alignment to junction candidates | 18:08:35 19 Dec 2020 | 18:09:39 19 Dec 2020 | 1 minute 4 seconds |
| Resolving best read alignments | 18:09:39 19 Dec 2020 | 18:10:17 19 Dec 2020 | 38 seconds |
| Creating BAM files | 18:10:17 19 Dec 2020 | 18:11:03 19 Dec 2020 | 46 seconds |
| Tabulating error counts | 18:11:03 19 Dec 2020 | 18:11:26 19 Dec 2020 | 23 seconds |
| Re-calibrating base error rates | 18:11:26 19 Dec 2020 | 18:11:31 19 Dec 2020 | 5 seconds |
| Examining read alignment evidence | 18:11:31 19 Dec 2020 | 18:17:22 19 Dec 2020 | 5 minutes 51 seconds |
| Polymorphism statistics | 18:17:22 19 Dec 2020 | 18:17:23 19 Dec 2020 | 1 second |
| Output | 18:17:23 19 Dec 2020 | 18:18:01 19 Dec 2020 | 38 seconds |
| Total | 17 minutes 5 seconds | ||
