Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,823,788 C→A L92M (CTG→ATG)  cho → endonuclease of nucleotide excision repair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,823,7880CA97.1% 233.2 / ‑3.3 68L92M (CTG→ATG) choendonuclease of nucleotide excision repair
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (39/27);  total (40/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACACCATGCCGCTCTATATCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAG  >  NC_000913_3_pae_pgi/1823646‑1823929
                                                                                                                                              |                                                                                                                                             
tGACACCATGCCGCTCTATATCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCt                                                                                                                                        >  1:697528/1‑150 (MQ=255)
 gACACCATGCCGCTCTATATCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCtt                                                                                                                                       <  2:805521/150‑1 (MQ=255)
    acCATGCCGCTCTATATCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGTATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGaa                                                                                                                                    <  4:281701/150‑1 (MQ=255)
    acCATGCCGCTCTATATCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGaa                                                                                                                                    <  1:876390/150‑1 (MQ=255)
     ccATGCCGCTCTATATCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAg                                                                                                                                   <  2:893586/150‑1 (MQ=255)
         gCCGCTCTATATCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATGAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGcgcg                                                                                                                               >  1:220907/1‑150 (MQ=255)
         gCCGCTCTATATCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGcgcg                                                                                                                               >  2:235306/1‑150 (MQ=255)
           cGCTCTATATCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGAt                                                                                                                             >  3:14757/1‑150 (MQ=255)
           cGCTCTATATCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGAt                                                                                                                             <  3:224764/150‑1 (MQ=255)
                  tatCGGCAAAAGCGTTAACATCCGCAGCCGCGGCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGCTTAATCaa                                                                                                                      >  2:434359/1‑150 (MQ=255)
                   atCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCaaa                                                                                                                     <  3:24849/150‑1 (MQ=255)
                    tCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCCCCTTGAAGCGCGATTAATCAAAg                                                                                                                    >  2:13880/1‑150 (MQ=255)
                     cGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGa                                                                                                                   >  3:497525/1‑150 (MQ=255)
                                  aaCATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTg                                                                                                      <  3:88151/150‑1 (MQ=255)
                                    cATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGtt                                                                                                    <  1:235306/150‑1 (MQ=255)
                                    cATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGtt                                                                                                    >  2:565143/1‑150 (MQ=255)
                                    cATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGtt                                                                                                    <  1:566629/150‑1 (MQ=255)
                                        cgcgGCCGCGTCGTTTCTCATTTACCGACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCTCCGGTTAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTtaat                                                                                                <  4:404376/150‑1 (MQ=255)
                                              cgcgTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACgg                                                                                          >  2:371832/1‑150 (MQ=255)
                                               gcgTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTACTAAACGGt                                                                                         >  2:254749/1‑150 (MQ=255)
                                                     tttCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGcgcc                                                                                   >  1:222667/1‑150 (MQ=255)
                                                     tttCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGcgcc                                                                                   >  1:670528/1‑150 (MQ=255)
                                                     tttCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGcgcc                                                                                   >  3:351034/1‑150 (MQ=255)
                                                       tctcATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAAc                                                                                            <  4:105448/139‑1 (MQ=255)
                                                       tctcATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAAc                                                                                            >  3:105448/1‑139 (MQ=255)
                                                           aTTTACGGACCCCGGATGAAGCCGCCGTGCTAAGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATc                                                                             <  4:348990/150‑1 (MQ=255)
                                                               aCGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCa                                                                         >  1:432571/1‑150 (MQ=255)
                                                               aCGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCa                                                                         <  2:313623/150‑1 (MQ=255)
                                                                cGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAg                                                                        <  4:454878/150‑1 (MQ=255)
                                                                     cccGGATGAAGCCGCCATGCTACGGCAATCCCAACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCtg                                                                   >  2:885984/1‑150 (MQ=255)
                                                                      ccGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGaa                                                                                                                                    >  3:281850/1‑84 (MQ=255)
                                                                      ccGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGaa                                                                                                                                    <  4:281850/84‑1 (MQ=255)
                                                                       cGGATGAAGCCGCCATGCTACGGCAATCCCGACGGCTCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAACGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCtgtg                                                                 >  1:781182/1‑150 (MQ=255)
                                                                       cGGATGAAGCCGCCATGCTACGGCAATAACGACGGACCAGCTGGATATGTACCGAAGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTACTCAAAGAACAACAGCCGCTGTCTAATCAACGGTTGCGCCGCAATCGCCAGCTCcgtc                                                                 >  2:39215/1‑149 (MQ=255)
                                                                          aTGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGccc                                                              <  1:577359/150‑1 (MQ=255)
                                                                               gccgccATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCaa                                                         >  2:35150/1‑150 (MQ=255)
                                                                                ccgccATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATc                                                                                                                        <  1:151879/86‑1 (MQ=255)
                                                                                ccgccATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATc                                                                                                                        >  2:151879/1‑86 (MQ=255)
                                                                                ccgccATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAAt                                                        <  4:159063/150‑1 (MQ=255)
                                                                                   ccATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTaa                                                     <  2:489157/150‑1 (MQ=255)
                                                                                    cATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTaaa                                                    <  2:670528/150‑1 (MQ=255)
                                                                                       gCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGa                                                 >  1:659255/1‑150 (MQ=255)
                                                                                       gCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGACGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGa                                                 >  1:320084/1‑150 (MQ=255)
                                                                                        cTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGaa                                                >  1:180596/1‑150 (MQ=255)
                                                                                        cTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGaa                                                >  1:249528/1‑150 (MQ=255)
                                                                                          aCGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGaaaa                                              >  2:875800/1‑150 (MQ=255)
                                                                                          aCGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTACCCTGCAATTAAATGaaaa                                              >  2:881407/1‑150 (MQ=255)
                                                                                           cGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAg                                             >  1:342417/1‑150 (MQ=255)
                                                                                              cAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAgcgc                                          <  1:779564/150‑1 (MQ=255)
                                                                                               aaTCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATGAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGcgcg                                         >  1:541520/1‑150 (MQ=255)
                                                                                                    cGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGAt                                    >  1:237023/1‑150 (MQ=255)
                                                                                                     gACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGAtg                                   >  2:509509/1‑150 (MQ=255)
                                                                                                             aGCTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTAt                           <  2:237023/150‑1 (MQ=255)
                                                                                                               cTGGATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGc                         >  1:328665/1‑150 (MQ=255)
                                                                                                                  gATATGTACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCaa                      >  1:424844/1‑150 (MQ=255)
                                                                                                                        tACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGt                <  1:565143/150‑1 (MQ=255)
                                                                                                                        tACCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGt                <  2:362425/150‑1 (MQ=255)
                                                                                                                        tACCGCCGGTGAAATCGGCGCTATGCGCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGt                >  3:568966/1‑150 (MQ=255)
                                                                                                                         aCCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTg               >  1:699051/1‑150 (MQ=255)
                                                                                                                         aCCGCCGGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTg               >  1:538956/1‑150 (MQ=255)
                                                                                                                           cgccgGTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGAAATCGCCAGCTTTGTGCCCTGCAATGAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGa             <  2:220907/150‑1 (MQ=255)
                                                                                                                                gTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACACCAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAAATAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGAttttt        <  4:14757/150‑1 (MQ=255)
                                                                                                                                gTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGAttttt        <  2:180596/150‑1 (MQ=255)
                                                                                                                                gTGAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGAttttt        >  1:72697/1‑150 (MQ=255)
                                                                                                                                  gAAATCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCa      >  4:248131/1‑150 (MQ=255)
                                                                                                                                     aTCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGa   <  3:248131/150‑1 (MQ=255)
                                                                                                                                      tCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAg  >  2:667884/1‑150 (MQ=255)
                                                                                                                                      tCGGCGCTATGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGAGGTGGTGTATGCCAAAGCGGTGGATGTTTCACGac  >  2:578307/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                             
TGACACCATGCCGCTCTATATCGGCAAAAGCGTTAACATCCGCAGCCGCGTCCTTTCTCATTTACGTACCCCGGATGAAGCCGCCATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAG  >  NC_000913_3_pae_pgi/1823646‑1823929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: