| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_pgi | 4,037,225 | 0 | A | . | 44.4% | 8.0 / 12.0 | 9 | intergenic (+8/‑35) | ileT/alaT | tRNA‑Ile/tRNA‑Ala |
| Reads supporting (aligned to +/- strand): ref base A (3/2); new base . (0/4); total (3/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.02e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACG‑GCAAATTTGAAG > NC_000913_3_pae_pgi/4037077‑4037239 | aaaGAAGCGTTCTTTGAAGTGCTCACACAGATGGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCACGTCCACTCAGGCCTACCAAATTTGCAc > 1:806927/1‑150 (MQ=21) agaagCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACg‑g < 4:146444/150‑1 (MQ=34) ttCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACG‑GCAAAttt > 1:1076184/1‑150 (MQ=34) ttCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGC‑CGTGCAAAttt < 2:257537/150‑1 (MQ=255) ttCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGC‑CGTGCAAAttt < 2:653479/150‑1 (MQ=255) tCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGC‑CGTGCAAATTTg < 2:242912/150‑1 (MQ=255) cTTTGAAGTGCTCACACAGAGTGTCTGATGAAAATGAGCAGTAAACCCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATCAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTCCCAAATTTGCACG‑GCAAATTTGa < 4:258420/150‑1 (MQ=255) tttGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACG‑GCAAATTTGaa > 1:133871/1‑150 (MQ=32) ttGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGC‑CGTGCAAATTTggta < 2:510158/150‑4 (MQ=255) | AAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACG‑GCAAATTTGAAG > NC_000913_3_pae_pgi/4037077‑4037239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |