Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_pgi 3,772,736 G→A 7.5% P152P (CCG→CCA)  mtlA → mannitol‑specific PTS enzyme: IIA, IIB and IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,772,7360GA 7.5% 143.8 / 3.4 57P152P (CCG→CCA) mtlAmannitol‑specific PTS enzyme: IIA, IIB and IIC components
Reads supporting (aligned to +/- strand):  ref base G (27/24);  new base A (2/2);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.21e-01

TCCGCTGGGCGGCTGGTGCATTAAGCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCACGGTATCTTCTCGCCGCTGG  >  NC_000913_3_pae_pgi/3772592‑3772884
                                                                                                                                                |                                                                                                                                                    
tCCGCTGGGCGGCTGGTGCATTAAGCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCAGGATAATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGAttg                                                                                                                                                  >  1:117140/1‑149 (MQ=255)
                tGCATTAAGCACTTCGACCGCTGGGTAGACGGTAAGATCAACTCCGGTTTTGAGATGCTGGTGGATACCTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATCCTGGCATTCCTCGGCCTTGCCCCCATTGTTGAAACCCtctcaca                                                                                                                                  >  2:531091/1‑146 (MQ=255)
                 gCATTAAGCAGTTCGACCGCTGGGTAGACGGAAAGATTAAATACGGTTTTGAGATGGCGGTGAGTCACATCTCCGCATGCATCATCGGGATGATCGTGGCGATGTTGGCGTTCCTCGACCTTGGCGCTGTTGTTGTAGCCGGGTCCaag                                                                                                                                 >  2:665203/1‑148 (MQ=255)
                   aTTAAGCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCAAGTTTTAAGATGATGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATACTGACATTCCGCGGCATTAGCCCGATTGTTGAAGCCCTGAGCAAAAt                                                                                                                               >  2:855430/1‑149 (MQ=255)
                        gCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCt                                                                                                                                        <  2:477045/135‑1 (MQ=255)
                        gCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCt                                                                                                                                        >  1:477045/1‑135 (MQ=255)
                        gCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATgctgg                                                                                                                          <  1:172204/149‑1 (MQ=255)
                        gCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCAATTGTTGAAGCCCTGTCCAAAATgc                                                                                                                             <  1:279822/146‑1 (MQ=255)
                        gCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCAATTGTTGAAGCCCTGTCCAAAATgc                                                                                                                             <  1:279821/146‑1 (MQ=255)
                        gCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCAATTGTTGAAGCCCTGTCCAAAATgc                                                                                                                             >  2:279822/1‑146 (MQ=255)
                        gCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCAATTGTTGAAGCCCTGTCCAAAATgc                                                                                                                             >  2:279821/1‑146 (MQ=255)
                          aCTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATGATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGTAGCCCTTTCCAAAATgctggct                                                                                                                        >  2:300825/1‑149 (MQ=255)
                                aCCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGTATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGc                                                                                                                  <  1:351064/149‑1 (MQ=255)
                                  cGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGt                                                                                                                <  2:112709/149‑1 (MQ=255)
                                  cGCTGGGTAGACGGTAAGATCAAATCCGGATTTGAGATGCTGGTGAATAACTTCTCCGCAAGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGACATTGGCCCGATTGTTGAATCCCTGTCCAAAATGCTGGCTgcgcgcct                                                                                                                >  2:817807/1‑147 (MQ=255)
                                         tAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTc                                                                                                         <  2:400713/149‑1 (MQ=255)
                                           ggcGGGAAGGTCAAATCCGGTTTTGAGGTGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCAt                                                                                                       <  2:442251/147‑1 (MQ=255)
                                             cGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATgg                                                                                                     >  1:804216/1‑149 (MQ=255)
                                               gTAAGATCGAATCCGGTTTTGAGATGGTGGTGGAGAGCTCCTCCGCAGGCAGCAGCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCCTTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGtt                                                                                                   <  1:887983/149‑1 (MQ=255)
                                               gTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCt                                                                                                                                        >  1:297493/1‑112 (MQ=255)
                                               gTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCt                                                                                                                                        <  2:297493/112‑1 (MQ=255)
                                                tAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCAGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGCCTTCGGGCGTTAACTTCATGGttt                                                                                                  >  2:36098/1‑148 (MQ=255)
                                                    aTCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCTCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCa                                                                                              >  2:993429/1‑149 (MQ=255)
                                                         aTCCGGTTATGAGCTGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATGCTGGCATTCCTCGGCAGTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACa                                                                                         <  1:891698/149‑1 (MQ=255)
                                                               ttttGAGATGCTAGTGCATAACTTCTCCGCAGGCATCATCGTGATGATCCTCGCTATTCTGGCATTCATCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCTTTAAGTTCATGGTTTGCCATGACAtgctcc                                                                                   >  2:120713/1‑147 (MQ=255)
                                                                     gaTGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTgg                                                                             >  1:648490/1‑149 (MQ=255)
                                                                     gaTGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTgg                                                                             >  1:565384/1‑149 (MQ=255)
                                                                         cTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATgg                                                                                                     >  2:475658/1‑121 (MQ=255)
                                                                         cTGGTGAATAACTGCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATgg                                                                                                     <  1:475658/121‑1 (MQ=255)
                                                                          tGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGCAGCCCTGTCCAAAATGCTGGCTGCGGGGGTTAACTTCATGGTTGTCCCTGACATGCTGCCGCTGGCGGCt                                                                        >  1:67511/1‑149 (MQ=255)
                                                                           ggTGAATAACTTATCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCGCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGACGTTAACTTAATGGTTGTCCATGACATGCTGCCGCTGGCGTCTa                                                                       >  2:627163/1‑149 (MQ=255)
                                                                                aTAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCttt                                                                  <  1:738453/149‑1 (MQ=255)
                                                                                     ttCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGa                                                             <  1:696926/149‑1 (MQ=255)
                                                                                      tctcCGCTGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGaa                                                            <  2:748629/149‑1 (MQ=255)
                                                                                          cGCAGGCATCATCGGGATGATCCTCGCCATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACTgg                                                        >  2:276286/1‑149 (MQ=255)
                                                                                               gCATCAGCGGGATGATCATCGCTATTCTGGCCTTCATCGGCATTGGACCGATTGTTGAAGCACAGTCCAAAATGCTGGCTGCGGGCGATAACTTCATGTTTGTGCAGGACATGCTGCCGCTGATGTCTAGATTTGGTGGACGGGAGaaa                                                   >  2:70604/1‑149 (MQ=255)
                                                                                                 atcatcGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAACTGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTAACTTTGTTGAACCGGCAAAAAt                                                 >  1:408422/1‑149 (MQ=255)
                                                                                                     tcGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCCAAAATCCTg                                             >  1:301197/1‑149 (MQ=255)
                                                                                                     tcGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGACTATCTTTGTTGAACCGGAGAAAATCCTg                                             >  1:101365/1‑149 (MQ=255)
                                                                                                        ggATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTTTCTTTTTTGAACCGGCGAAAATCCTGTTc                                          >  1:415975/1‑149 (MQ=255)
                                                                                                        ggATGATCATCGCTATGCTGGTAGTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGTTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTc                                          <  1:490320/149‑1 (MQ=255)
                                                                                                         gatgatCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTAt                                                                      >  1:775838/1‑120 (MQ=255)
                                                                                                         gatgatCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTAt                                                                      <  2:775838/120‑1 (MQ=255)
                                                                                                          atggtCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCt                                        <  2:118550/149‑1 (MQ=255)
                                                                                                                   gCTATCCTGCCATTCTTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGc                               <  2:456042/149‑1 (MQ=255)
                                                                                                                     tATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCa                             <  2:51616/149‑1 (MQ=255)
                                                                                                                            gCATTCCTCGGCATTGGCCCGTTTGTTGAAGCCCTTTCCAAAATGCTGGCTGCGGTCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTAGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCa                      <  1:217372/149‑1 (MQ=255)
                                                                                                                             cATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGTTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCAc                     <  1:461238/149‑1 (MQ=255)
                                                                                                                              ataccTCGGCATTGGCCGGATTGTTTAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCACg                    <  2:159679/146‑1 (MQ=255)
                                                                                                                                tCCTCGGCATTGGCCTGATTGTTGAGGCCCTGTCCAAAAGCCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCACGGt                  <  1:636127/149‑1 (MQ=255)
                                                                                                                                      gCATTGGCCCGATTGTTGAAGTCTTTTTCTAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCACGGTAtcttc            <  1:357893/149‑1 (MQ=255)
                                                                                                                                         ttGGCCGGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCATGTTCCTAAACAACTCCATTAACCACGGTATCTTCTcg         >  2:460224/1‑149 (MQ=255)
                                                                                                                                         ttGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCACGGTATCTTCTcg         >  1:680241/1‑149 (MQ=255)
                                                                                                                                           ggCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCACGGTATCTTCTcgcc       >  2:828922/1‑149 (MQ=255)
                                                                                                                                             cccGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCACGGCATCTTCTcgccgc     >  1:562975/1‑149 (MQ=255)
                                                                                                                                              ccGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGAGTTAACTTCATGGTTGTCCATGAAATGCTGCCGCTTGCGTCTGACTTTGTTGAACCGGCGAAGATCCTGTTCCTAAACGACGCCATTAACCAAGGTATCTTATcgccgcc    >  2:13439/1‑148 (MQ=255)
                                                                                                                                               cGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCACGGTATCTTCTCGCCGCTg   <  2:580910/149‑1 (MQ=255)
                                                                                                                                                gATTGTTGAAGCCCTGTGCAAAATGCTTGGTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCACGGTATCTTCTCGCCGCTgg  <  2:544863/149‑1 (MQ=255)
                                                                                                                                                gATTGTTGAAGCCCTGTCCAAAATGCTGGCTGTGTGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCACGGTATCTTCTCGCCGCTgg  <  1:179341/149‑1 (MQ=255)
                                                                                                                                                gATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGTCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCCCGGTACCTTGTTGCCGCggg  <  2:874800/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TCCGCTGGGCGGCTGGTGCATTAAGCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCTCCGCAGGCATCATCGGGATGATCCTCGCTATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATGCTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCGCTGGCGTCTATCTTTGTTGAACCGGCGAAAATCCTGTTCCTCAACAACGCCATTAACCACGGTATCTTCTCGCCGCTGG  >  NC_000913_3_pae_pgi/3772592‑3772884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑