Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_pgi 2,872,915 C→G 11.8% intergenic (‑95/+99) ftsB ← / ← ygbE cell division protein/DUF3561 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi2,872,9150CG11.8% 103.6 / 7.4 41intergenic (‑95/+99)ftsB/ygbEcell division protein/DUF3561 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (15/21);  new base G (3/2);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01

GAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTG  >  NC_000913_3_pae_pgi/2872768‑2873060
                                                                                                                                                   |                                                                                                                                                 
gAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCAGCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACca                                                                                                                                                  <  2:863592/149‑1 (MQ=255)
  aTACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGACAATTGTACcaca                                                                                                                                                >  2:47029/1‑149 (MQ=255)
   tACTGGAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATGATCCCATGCAGCGGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACAt                                                                                                                                               <  2:414849/149‑1 (MQ=255)
     cTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGCCGACTCTACATCCTATGTTGTGGATACCGGGACAACGCGCGCAACTGTTAGACAttt                                                                                                                                             >  2:144388/1‑149 (MQ=255)
        ttgcaagaccagaATAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTGCTCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTc                                                                                                                                          <  1:459125/144‑1 (MQ=255)
            cagaccagaATAGCCGGGAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAGACGGCGTCATCATCCCATGCATCCGACGATGCCTTTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCACATGTACCACATTTGTCCAtt                                                                                                                                      <  2:253030/149‑1 (MQ=255)
            cagaccagaATAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGAAAAAGTGGGGAAATGTACCACTTTTGTCCAtt                                                                                                                                      >  2:709038/1‑149 (MQ=255)
              gaccagaATAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGAGACAACGCGGGCAAATGTACCACATTTATCCATTGt                                                                                                                                    >  2:643280/1‑149 (MQ=255)
                    aaTAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATACGAAGCCGACTCTACATCCTCTGTTGAGGATACCGCGACAACGCGGGCAAATGTGCCACATTTGTCCATTGTTACTta                                                                                                                              >  2:197715/1‑149 (MQ=255)
                    aaTAACCAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGta                                                                                                                              >  2:988234/1‑149 (MQ=255)
                        gCCATCAACAGCAGCGTGAGTTTACCCATCCTGCGCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATAcc                                                                                                                          <  2:838837/149‑1 (MQ=255)
                          cAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACAACTATACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACATATACCCa                                                                                                                        >  1:49299/1‑149 (MQ=255)
                               aCAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGtt                                                                                                                                   <  2:544115/133‑1 (MQ=255)
                               aCAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGtt                                                                                                                                   >  1:544115/1‑133 (MQ=255)
                                      cGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGaa                                                                                                            <  1:278669/149‑1 (MQ=255)
                                      cGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGaa                                                                                                            <  2:705234/149‑1 (MQ=255)
                                      cGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGAGTGCAGaa                                                                                                            >  1:626232/1‑149 (MQ=255)
                                             ttactactCCTGCCCCCTGAAAAACCGCATCATCATCCCATGCAGCCGAAGACGACTCTACATCCTCTGTCGGGGAGACCGCGACAACGCGGGCAAAGGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAAtc                                                                                                     <  2:1005169/142‑1 (MQ=255)
                                                 ctaTCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGTTTCTACATCTTTTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTACATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCat                                                                                                 <  1:355649/147‑1 (MQ=255)
                                                  cgtctggccgccTGAAAAACGGCAGTATCAGCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCatt                                                                                                <  2:396801/143‑1 (MQ=255)
                                                      cTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCAttatta                                                                                            <  2:587692/149‑1 (MQ=255)
                                                          ccccTGAAAAATGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATCCCGCGACAAGGCGGGAAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATACTCTTATTATTAGTTa                                                                                        <  2:726119/149‑1 (MQ=255)
                                                          ccccTGAAAAACGGCATTAGCATCCCATGCATCCGAAGATGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTa                                                                                        <  1:381956/149‑1 (MQ=255)
                                                               gAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGtt                                                                                   <  1:514667/149‑1 (MQ=255)
                                                                        catcatcatCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATg                                                                          <  1:613159/149‑1 (MQ=255)
                                                                          tcatcatcCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGaa                                                                        <  1:903885/149‑1 (MQ=255)
                                                                          tcatcatcCCATGCATCCGAAGACGACTCTACATCCGCTGTTGAGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGaa                                                                        <  1:830573/149‑1 (MQ=255)
                                                                            atcatcCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCAATATTAGTTACGGTATGAATTATGAACa                                                                      >  1:300502/1‑149 (MQ=255)
                                                                                    aTGCATCCGAAGACGACTCTAAATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGAc                                                              >  2:885674/1‑149 (MQ=255)
                                                                                     tGCATCCGAAGACGACTCTACATCCTCTGGTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACg                                                             <  1:706406/149‑1 (MQ=255)
                                                                                               gacgacTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACa                                                   <  1:936866/149‑1 (MQ=255)
                                                                                                  gacTCTACATCCTCTGTTGGGGATACCGCGACGACGCGGGCAAATGTACCACATTTGTCCATTGTTAGGTATAGCCAAGGCGTGCAGAACATAATTTCATTATTAGTGAAGTATTTAATTATGAACAGAGGAGACGGGAAAGTaacaat                                                >  2:323745/1‑144 (MQ=255)
                                                                                                  gacTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACGACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGaa                                                                               <  1:848826/118‑1 (MQ=255)
                                                                                                  gacTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACGACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGaa                                                                               <  1:848665/118‑1 (MQ=255)
                                                                                                  gacTCTACATCCTCTGTTGAGGATACCGCGACAACGCGGGCAAATGTACGACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGaa                                                                               >  2:848826/1‑118 (MQ=255)
                                                                                                  gacTCTACATCCTCTGTTGAGGATACCGCGACAACGCGGGCAAATGTACGACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGaa                                                                               >  2:848665/1‑118 (MQ=255)
                                                                                                         cATCCTATGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCa                                         <  2:808010/149‑1 (MQ=255)
                                                                                                             ctctGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGTAg                                     >  2:585918/1‑149 (MQ=255)
                                                                                                             ctctGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGAAGACGGGAAAGTACAAATTAGCCCAGTAg                                     >  1:753301/1‑149 (MQ=255)
                                                                                                                       gATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGTAGCCACATAAAc                           >  1:548578/1‑149 (MQ=255)
                                                                                                                        aTACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGTAGCCACATAAACa                          >  2:652205/1‑149 (MQ=255)
                                                                                                                                              tGTACCACATTTGTCCATTGTTACTTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAAGGGTCGGGAAAGTACAAATGATCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTAc    <  1:144388/149‑1 (MQ=255)
                                                                                                                                                tACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTTAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAATAGCCACATAAACAGTGAGCAAAAGATAATTCATACtt  >  1:170452/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTG  >  NC_000913_3_pae_pgi/2872768‑2873060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑