Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_pgi 2,436,694 G→A 6.6% intergenic (‑45/+21) usg ← / ← pdxB putative semialdehyde dehydrogenase/erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi2,436,6940GA 6.6% 170.1 / 2.4 61intergenic (‑45/+21)usg/pdxBputative semialdehyde dehydrogenase/erythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (28/29);  new base A (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

TTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTA  >  NC_000913_3_pae_pgi/2436550‑2436841
                                                                                                                                                |                                                                                                                                                   
tttCCCCAGCCGGGAACTGACGTTCAGCCAGCGTTTCCAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCaga                                                                                                                                                 <  1:815816/149‑1 (MQ=255)
   ccccAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGAGGTTTACTCCAGAAATAGCAAAGCTCCCGGCCAATGTTACAGCagagag                                                                                                                                              <  1:288857/149‑1 (MQ=255)
    cccAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCATAGCGCCAGTTGCGCCCAGGACGTCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAACAAAGCTCCCTGCCAATGTTACAGCAGAGAGc                                                                                                                                             >  1:62691/1‑149 (MQ=255)
    cccAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAACTACCTGCCAATGTTACCGCAGAGGGc                                                                                                                                             >  2:696813/1‑149 (MQ=255)
     ccAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCa                                                                                                                                            >  2:600936/1‑149 (MQ=255)
      cAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGTCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCAt                                                                                                                                           >  1:660982/1‑149 (MQ=255)
       aaCCGGGAACTGACGTTCAGCCAGCGTTTCAAGCCGGGCTTCGCCCACAGCGCCAGTTGAGCCCAGGACGGCCATGTTCAAGCCTTCAGACAAGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCAtt                                                                                                                                          >  2:895097/1‑148 (MQ=255)
         ccGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAACAGAGAGCATgaa                                                                                                                                        >  2:816794/1‑149 (MQ=255)
         ccGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAACAGAGAGCATgaa                                                                                                                                        >  2:816913/1‑149 (MQ=255)
           gggACCTGCCGGCCAGCCAGCTTGTCAAGCAGGGCTTCGCCCACAGCGCCAGTGGCGCCCAGGACGGCAATGTTCCAGCAGTCACACCTAGTGGTTTACGCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATgacga                                                                                                                                      <  2:337285/149‑1 (MQ=255)
                  gACGTTCAGCCAGCGTTTCAAGCCAGTCTTCGCCCACAGCGCCAGTTGCGCCCAGTACGGCAATGTTCCAGTCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGAtt                                                                                                                               <  2:860461/149‑1 (MQ=255)
                    ctttCAGCCAGCGTTTCAAGCCGGGGTTGGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCCGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTaa                                                                                                                             <  2:564042/147‑1 (MQ=255)
                     gTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAAc                                                                                                                            <  2:59193/149‑1 (MQ=255)
                       tCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGt                                                                                                                          <  2:634017/149‑1 (MQ=255)
                       tCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGt                                                                                                                          <  1:414538/149‑1 (MQ=255)
                           ccaccGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTAACAGCAGAGAGCATGAAGAAGCGATTAACGTCCCg                                                                                                                      >  2:376256/1‑149 (MQ=255)
                                gTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAACCAGAGATTAACGTGCCGgctga                                                                                                                 >  2:54004/1‑149 (MQ=255)
                                     aaGCAGGGCTTCGCCCACCGCTCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGATATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAAc                                                                                                            <  2:83174/149‑1 (MQ=255)
                                      aGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGACATAGCAAAGCTCCCTGCCAAGGTTACAGCAGAGAGCATGAAGAAGAGACTAACGGGCCGGATGAGGAACg                                                                                                           >  2:754220/1‑149 (MQ=255)
                                          gggCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTCTCCTCCAGAAATAGCAAAGCTACCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCga                                                                                                       >  1:785473/1‑148 (MQ=255)
                                             cTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTaa                                                                                                    >  2:941620/1‑149 (MQ=255)
                                              ttCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTCACGTGCCGGATGATGAACGGCGTTaaa                                                                                                   >  2:85554/1‑149 (MQ=255)
                                                   ccaacgcgcCAGTTGCGGCCAGTACGGAAATGGTCCAGCTTTCAGACATGGTGGTTTACTCCAAAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAACATAACAGGGTGCTGGATTATGACCGGCGTTAAAACCCa                                                                                              <  2:467953/144‑1 (MQ=255)
                                                   ccACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCa                                                                                              <  1:860124/149‑1 (MQ=255)
                                                   ccACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAACATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCa                                                                                              >  2:420722/1‑149 (MQ=255)
                                                        gcgtCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATCGCGAAGCTCCCTGCCAATGTTATAGCAGAGAGCATGAAGCAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTa                                                                                         <  2:907786/149‑1 (MQ=255)
                                                          gcCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTCTGCGCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTaca                                                                                       <  1:190690/149‑1 (MQ=255)
                                                          gcCAGTTGCGCCCAGGACGGAAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTaca                                                                                       <  2:60739/149‑1 (MQ=255)
                                                          gcCAGGTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTaca                                                                                       <  1:345671/149‑1 (MQ=255)
                                                            gatgtGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTAcaca                                                                                     <  1:224314/145‑1 (MQ=255)
                                                            cAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGgat                                                                                                                   >  1:180936/1‑119 (MQ=255)
                                                            cAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGgat                                                                                                                   <  2:180936/119‑1 (MQ=255)
                                                             aGTTGATCCCAGTACGTCAATGTTCCAGCCTTCAGACAAGGTGGTTTACTCCAGAACTAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAg                                                                                    <  1:285558/149‑1 (MQ=255)
                                                                             gCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGCAAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTg                                                                    >  1:237704/1‑149 (MQ=255)
                                                                             gCAATGTTCCAGCCTTAAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTg                                                                    <  1:643464/149‑1 (MQ=255)
                                                                              cAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGAAGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTgg                                                                   >  1:35587/1‑149 (MQ=255)
                                                                               aaTGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGc                                                                  >  2:72395/1‑149 (MQ=255)
                                                                                aTGTGCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCg                                                                 <  2:918182/149‑1 (MQ=255)
                                                                                 tGTTCCAGCCTTCAGACATGGTGGTTTACTCAACACATCGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCAGGAAGAAGAGATTAACGTGCCGGATGGTGAACGGCGTTAAAACCCAGTTTTCACACCACTGACGCCCCACTGGcac                                                                >  2:192220/1‑147 (MQ=255)
                                                                                   ttCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGtca                                                              >  1:81273/1‑149 (MQ=255)
                                                                                   ttCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGAAATGATGCCGCACTGGCGcca                                                              >  1:124318/1‑149 (MQ=255)
                                                                                     ccAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGtcatc                                                            >  1:358454/1‑149 (MQ=255)
                                                                                     ccAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGtcatc                                                            <  1:600936/149‑1 (MQ=255)
                                                                                          ctgcAGACATGGTGGTTTAGTCCATAAATAGCAAAGCTCCGTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACaaa                                                       <  2:441449/146‑1 (MQ=255)
                                                                                          cTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCAACCTGCCAATGTTACAGCAGAGAGCATGAAGAAGTGATTAACGTGCCGGATAATGAACGGAGTTAAAACCCAGTTTACACAGCAATGAGGCCGCACTGGCGTCATCACaaa                                                       >  2:355/1‑149 (MQ=255)
                                                                                             cAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATta                                                    >  1:856541/1‑149 (MQ=255)
                                                                                               gACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATtaca                                                  <  1:643354/149‑1 (MQ=255)
                                                                                                 cATGGGGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATtacata                                                <  2:957749/149‑1 (MQ=255)
                                                                                                 aagtgtgggTTACTCCAGAAATAGCAAAGCTCCCCGCCAATGTTACAGCAGAGAGAATGAAGAAGAGATTAACGTTCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATtacata                                                <  2:197552/145‑1 (MQ=255)
                                                                                                   tggtggTTTACTCCAGAAATAGCAAGGCTCCCTGCCAATGTTATAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTCGAGTCACCACAAATtacataca                                              <  2:888218/149‑1 (MQ=255)
                                                                                                    ggtggtTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACag                                             >  1:114864/1‑149 (MQ=255)
                                                                                                               ccAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCAtt                                  <  1:974959/149‑1 (MQ=255)
                                                                                                                 aGAAATAGCGAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACACCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGGCCAttag                                >  2:499372/1‑147 (MQ=255)
                                                                                                                  gAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCAc                               <  1:72395/149‑1 (MQ=255)
                                                                                                                              cTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGCCGCTCAAAAt                   >  1:801399/1‑149 (MQ=255)
                                                                                                                                 ccTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCACCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGt                >  1:480450/1‑149 (MQ=255)
                                                                                                                                 ccTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGGCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGt                >  1:530989/1‑149 (MQ=255)
                                                                                                                                   tGCCAATGTTACAACAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGttt              <  1:816794/149‑1 (MQ=255)
                                                                                                                                   tGCCAATGTTACAACAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGttt              <  1:816913/149‑1 (MQ=255)
                                                                                                                                     ccAATGTGACGGGAGAGAGATTGAGGAAGAGAATATCGTTTCTGTTTATGTACGCCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACTAATTCCTTACAGAGATGACCATTCACGGCGCTCAAGATAGttttt            <  2:53240/149‑1 (MQ=255)
                                                                                                                                        aTGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTgcg         >  2:276698/1‑149 (MQ=255)
                                                                                                                                               agcagAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAGACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACGGAGTTGACCATTCGCGGTGTTGAAGTTTGTTTTTTCGTAGttga  >  1:66451/1‑147 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTTGCGCCCAGGACGGCAATGTTCCAGCCTTCAGACATGGTGGTTTACTCCAGAAATAGCAAAGCTCCCTGCCAATGTTACAGCAGAGAGCATGAAGAAGAGATTAACGTGCCGGATGATGAACGGCGTTAAAACCCAGTTTACACAGCAATGATGCCGCACTGGCGTCATCACAAATTACATACAGAGATGACCATTCACGGCGCTCAAGATAGTTTTTGCGCAGTTTA  >  NC_000913_3_pae_pgi/2436550‑2436841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑