Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_pgi 1,529,203 C→A 100% pseudogene (1302/2037 nt) rhsE → pseudogene, Rhs family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,529,2030CA100.0% 26.3 / NA 15pseudogene (1302/2037 nt)rhsEpseudogene, Rhs family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (7/8);  total (7/8)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGG  >  NC_000913_3_pae_pgi/1529161‑1529348
                                          |                                                                                                                                                 
acCGGCGCGAAAAGCTAATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGc                                         <  1:20991/149‑1 (MQ=11)
   ggCGCGAAAAGCTCATCTTTATAACTGCGACCAACGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGt                                      <  2:209830/149‑1 (MQ=11)
         aaaaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGCGCAACCAGCTTAATGAGGAGAACCCGCCTCACCTGGACCAGCAGTACCGTc                                >  2:356192/1‑149 (MQ=11)
              ctCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTCTAATGAATGGGGCAACCAGCCTACTGAGGAGGACCCGCATCACCTGCCCCAGCCGTCCCGTCTGTCa                           >  2:923197/2‑149 (MQ=11)
                cATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACGAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAgg                         <  2:945737/149‑1 (MQ=11)
                 atctttATCACAGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAACCGCATCACCTGCACCAGCCGTACCGTCTGCCAggg                        <  1:404454/146‑1 (MQ=11)
                  tctttATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGgc                       >  1:811036/3‑149 (MQ=255)
                     ttATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACACGCATAACCTGCACCAGCCGTACCGTCTGCCAGGgcaga                    >  1:251293/1‑148 (MQ=14)
                                accaccGGGGACTGCCGGTGGCGGTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATgag         <  2:869742/149‑1 (MQ=255)
                                  caccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCACGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATgagga       <  2:782337/149‑1 (MQ=255)
                                  caccGGGGACTGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAAAGGCAAAACCAGCTTAATGGGTAGAATCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGTAGCATGATgagga       <  2:864707/149‑1 (MQ=14)
                                   accGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATgaggag      >  2:700152/1‑149 (MQ=255)
                                    ccGGGGACTGCAGCGGACGCTTATCAGCGAAGACGGCAATACGGCATGGCGCGGGGAGTATGGTGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGCGGGGt     >  2:907162/1‑149 (MQ=14)
                                     cGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTc    >  1:383354/1‑149 (MQ=255)
                                       gggACTGCCGCTGGCGCTTATCAGCGAAGATGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCGTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCgg  <  1:721767/149‑1 (MQ=255)
                                          |                                                                                                                                                 
ACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGG  >  NC_000913_3_pae_pgi/1529161‑1529348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑