Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 2,513,351 C→A L104M (CTG→ATG)  nupC → nucleoside (except guanosine) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi2,513,3510CA95.2% 196.6 / ‑2.1 62L104M (CTG→ATG) nupCnucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (19/40);  total (19/43)
Fisher's exact test for biased strand distribution p-value = 5.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TTCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACTTT  >  NC_000913_3_KO_pgi/2513207‑2513494
                                                                                                                                                |                                                                                                                                               
tttgggAAAGGCTTCTCCGAAATGTTCGAAAAACTTCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGAt                                                                                                                                             <  1:158908/144‑1 (MQ=255)
ttCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGAt                                                                                                                                             <  2:165876/149‑1 (MQ=255)
ttCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGAt                                                                                                                                             <  1:424499/149‑1 (MQ=255)
          gcgtctcCGAAATGTTCGAAAAACTGCTCGGAGTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATtctc                                                                                                                                   <  2:244472/146‑1 (MQ=255)
            ttCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCa                                                                                                                                 <  2:609105/149‑1 (MQ=255)
            ttCTACGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCa                                                                                                                                 <  2:157032/149‑1 (MQ=255)
             tctcCGAAATGTTCGAAAAACTGCTCGGATGTGCCAAAGAAGTGACTAACTTCGTCTTTGGTAGAATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAAGCGTCTTTATCTCTGCAATGATCGGTATTCTCCAg                                                                                                                                <  2:727046/149‑1 (MQ=255)
               tcCGAAATGTTCGAAAGACTGCTGGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTGGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGca                                                                                                                              <  2:98233/149‑1 (MQ=255)
                 cGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGcaca                                                                                                                            <  1:880604/149‑1 (MQ=255)
                 cGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCACcaca                                                                                                                            >  2:853392/1‑149 (MQ=255)
                   aaaTGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACAtt                                                                                                                          <  1:5694/149‑1 (MQ=255)
                        ttCGAAAAACTTCGCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCgt                                                                                                                     <  2:490055/149‑1 (MQ=255)
                        ttCGAAAAACTGCTTGGATTTGCCAACGAAGGGACTAACTTTGTCTTTGGTAGCATGAATGATCAAGGTCTGGCATTTTTTTTCCTGATAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCgt                                                                                                                     <  2:129776/149‑1 (MQ=255)
                        ttCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCgt                                                                                                                     <  2:585890/149‑1 (MQ=255)
                          cGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCgtgt                                                                                                                   >  1:96408/1‑149 (MQ=255)
                             aaaaCTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGc                                                                                                                <  1:527695/149‑1 (MQ=255)
                                 cTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGt                                                                                                            >  1:254137/1‑149 (MQ=255)
                                  tgttCGGATTTGTCAATGAAGGTATTAACTTCGTCTTTGGTATCATGAATGATCAAGGCCTGGCATTATTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCGGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTg                                                                                                           <  1:366430/146‑1 (MQ=255)
                                  tGCTCGGATTTGCCAACGAAGGGACTTACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTg                                                                                                           <  1:243691/149‑1 (MQ=255)
                                   gCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCACAATCGTCGTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGa                                                                                                          <  2:643885/149‑1 (MQ=255)
                                           ttGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGACTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCcgc                                                                                                  <  2:790786/149‑1 (MQ=255)
                                                  cGAAGGAACTAACTTCGGCTTTGGTAGCATGAACGATCACGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGCTTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTg                                                                                           <  1:302670/149‑1 (MQ=255)
                                                   gAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGAATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGAGTATTGCCGGTGCTGATCCGCACACttac                                                                                          >  2:341666/1‑147 (MQ=255)
                                                    aaGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTCGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGt                                                                                         <  1:119223/149‑1 (MQ=255)
                                                       ggACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTc                                                                                      <  2:557377/149‑1 (MQ=255)
                                                         acttacttCGTCTTTGGTAGCATGTATGATCAAGGCCTGGCATTCTTCTTCCTGAATGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCTTCCGCGCAATTGGTTTCCt                                                                                    <  2:765304/149‑1 (MQ=255)
                                                         aCTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCt                                                                                    <  1:202206/149‑1 (MQ=255)
                                                          cTAACTTCGTCTTGGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTg                                                                                   <  1:317952/149‑1 (MQ=255)
                                                              ccgcGTCTTTGTGAGCATGAATGATCAGGGCCTGGCATTCTTCTGCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGctct                                                                               <  2:816785/146‑1 (MQ=255)
                                                                   tccttGGTAGAATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCGGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTTCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaaa                                                                          <  2:903568/146‑1 (MQ=255)
                                                                   tCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaaa                                                                          <  1:13760/149‑1 (MQ=255)
                                                                   tCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCATGCtctcacaa                                                                          >  1:407728/1‑145 (MQ=255)
                                                                   tCTTTGGTAGCATGAAGAATCAAGGCCTGGCATGCTTCTTCCTGAAAGTGGTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaaa                                                                          <  2:393949/149‑1 (MQ=255)
                                                                         gTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAAc                                                                    <  1:817264/149‑1 (MQ=255)
                                                                          tAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCCAAATTCGTTTCCTGCTCTCCAAAGTCAACg                                                                   >  2:637264/1‑149 (MQ=255)
                                                                            gCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGc                                                                 <  1:466715/149‑1 (MQ=255)
                                                                                gaatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCTTCCGCGCAATTGGCTTCCTGCTCTCCAAAGTCAACgaccggg                                                             >  2:498116/1‑143 (MQ=255)
                                                                                gaatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATgg                                                             <  1:776771/149‑1 (MQ=255)
                                                                                gaatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATgg                                                             <  1:585621/149‑1 (MQ=255)
                                                                                 aatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATggg                                                            <  1:542131/149‑1 (MQ=255)
                                                                                        aaGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTg                                                     >  1:125201/1‑149 (MQ=255)
                                                                                          ggCCTGGTAGTCTTGTTCTCGTAAGGGCTGTGTCCAATCGTCTTTATCTCTGCGATGATCGGTATTCTCCAGCACATGCGCGTGTTGTCGGTGATCATCCGCGCAATGGGTTTCCTGCTCTGCAAAGGCAACGGCATGGGCAAACTGGa                                                   <  2:482086/149‑1 (MQ=255)
                                                                                                  atccttcttcCTGAAAGTGCTGTGCCCAAGCGTCTTTATCTCTGCAATGATAGGTATTCTCCAGCACATTGGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGGCAACGGCATGGGCAAACTGGAATCCTTTa                                           <  2:884334/146‑1 (MQ=255)
                                                                                                  aTTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTa                                           >  1:734174/1‑149 (MQ=255)
                                                                                                  aTTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTa                                           <  1:383692/149‑1 (MQ=255)
                                                                                                    tcttcttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAAc                                         <  1:604735/149‑1 (MQ=255)
                                                                                                      ttcttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGc                                       >  2:449008/1‑149 (MQ=255)
                                                                                                        cttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTGGCGTGTTGCCGTTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCg                                     >  2:144524/1‑149 (MQ=255)
                                                                                                                        tgCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCtt                                             >  2:212192/1‑125 (MQ=255)
                                                                                                                        tgCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCtt                                             <  1:212192/125‑1 (MQ=255)
                                                                                                                         gcacAATCGTCTTGTTCTCTGCAATGATCGGTATTGTCCAGCACATTCGCGTGTTGCCGGTGATCATTCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGTTCCCTGATTCTg                    <  2:753867/146‑1 (MQ=255)
                                                                                                                          cccAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTgg                   >  2:540722/1‑149 (MQ=255)
                                                                                                                            cAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCACCACATTCGCGTGTTGCCGGTGAGCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGt                 <  1:384126/149‑1 (MQ=255)
                                                                                                                             aaTCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTc                >  1:119445/1‑149 (MQ=255)
                                                                                                                             aaTCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTc                >  1:628210/1‑149 (MQ=255)
                                                                                                                               tCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCATCAGCTCCCTGATTCTGGGTCAg              >  1:597906/1‑149 (MQ=255)
                                                                                                                               tCGTCTTTATCTCTGCAATGATCCATGCTTTTGGGCACATTCGCGTGTTGCCGGTGATCATACGCGCAATTGATTTCCTGCTCTCCAAAGTCAAAGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCCTCCTGATTCTGGGTCAg              >  2:420441/1‑149 (MQ=255)
                                                                                                                                  tCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCt           >  1:653895/1‑149 (MQ=255)
                                                                                                                                        tctcTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAAc     <  2:125201/149‑1 (MQ=255)
                                                                                                                                           ctGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACttt  <  2:863960/149‑1 (MQ=255)
                                                                                                                                            tGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGACTCCt                                              >  1:120300/1‑104 (MQ=255)
                                                                                                                                            tGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCt                                              <  2:120300/104‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TTCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACTTT  >  NC_000913_3_KO_pgi/2513207‑2513494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑