Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913_3_KO_pgi	4,233,757	+ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC	intergenic (‑524/‑583)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 4233757	0 (0.000)	40 (1.160) +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC	36/194	0.0	100%	intergenic (‑524/‑583)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
	?	NC_000913_3_KO_pgi	4233758 =	0 (0.000)					intergenic (‑525/‑582)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

TAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/4233629‑4233757 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCG  >  NC_000913_3_KO_pgi/4233758‑4233847                                                                                                                                  ||||||||||||||||||||||||||||||||||||||||||||||                                                                                           TAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAC                                                                                                                      <  1:328783/149‑1   AATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGCCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACac                                                                                                                    >  2:24809/1‑147        TTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACC                                                                                                               >  1:13817/1‑149           ACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAAACCGCT                                                                                                            >  1:152018/1‑149             TGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTTTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTAAAAATCAGAAGAGTATTGCTAATGAAAAAAATAAATCCAACGCAGACCGCTtc                                                                                                          >  2:51824/1‑147                   AAGGGTTTACACTCAACATTACGCTAACGGCCCTCACCCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCA                                                                                                    <  1:246692/149‑1                   AAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTCTCGCCGATCAAAAACATCAATCCAACGCAGACCGCTGCCTGGCA                                                                                                    <  1:440525/149‑1                   AAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCA                                                                                                    <  2:393466/149‑1                             ACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAAACCGCTGCCTGGCAGGCACTACGG                                                                                          >  2:706671/1‑149                              CTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGT                                                                                         >  1:3318/1‑149                               TCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTt                                                                                        >  2:290509/1‑148                               TCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTG                                                                                        >  2:111414/1‑149                               TCAACATTACGCTAACGGCACTAAAACAATCAAATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGAACTACGGTG                                                                                        >  1:123557/1‑149                                   CATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGG                                                                                    <  1:341297/149‑1                                         GCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAG                                                                              >  1:788952/1‑149                                               GGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTTCTAATGAAAAACATCAATCCAACGCAGACCGCTCCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTT                                                                        >  2:251545/1‑149                                                  ACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCCCAAAATCAGAAGAGTATTGCTAAAGAACACCATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGA                                                                     <  1:870704/149‑1                                                  ACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGACACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATACAAAGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGCCTGGACCTGCTTCGA                                                                     >  2:752742/1‑149                                                   CTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAA                                                                    <  1:507993/149‑1                                                    TAAAACCATCACATTTTTCTGTGACTGCCGCTAACCCCACCTATAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGTTGTAGGCTGGGGCTATTTAGAAG                                                                   >  2:153181/1‑149                                                     aaactCATCACATTTTCATGTGCCTGGCGCCACAATCTTCCAAAGTAACAATTCTCAAAATCAGAAGAGTATTGCTAATGAACCACCTGAAGCCTACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGT                                                                  <  1:302922/144‑1                                                       AACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATCATCACAACAAGAAGAGTATTGGTAATGAAGAACATCATGCCGGCGCAGTCCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTGCGAAGTTC                                                                <  1:178298/149‑1                                                           ATCACTTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATTAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGGAGTCTGGAGCTGCTTCGAAGTTCCTAT                                                            <  2:104850/149‑1                                                            TCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATA                                                           >  1:240365/1‑149                                                             CACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATAC                                                          <  1:326553/149‑1                                                                ATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTCTACTTT                                                       >  1:422953/1‑149                                                                  TTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCT                                                     <  1:517336/149‑1                                                                   TTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGTGACGTCGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTA                                                    <  1:666642/149‑1                                                                    TTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAG                                                   <  2:672117/149‑1                                                                         TGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAAT                                              <  1:12056/149‑1                                                                           ACTGGCGCTACTATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAACGAAAACCATCAATCCAACGCAGACCACGGCCTGCCAGGCACTACGTTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGACAATAG                                            <  2:184603/149‑1                                                                            CTGGCTCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGG                                           <  1:443820/149‑1                                                                            CTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAAT                                                                                                                                        <  2:865619/56‑1                                                                            CTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAAT                                                                                                                                        >  1:865619/1‑56                                                                             TGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATCGGA                                          >  2:471216/1‑149                                                                             TGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTACAGAATAGGA                                          >  2:557340/1‑149                                                                                CGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACT                                       <  1:906968/149‑1                                                                                  CTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGACGTGAGCTGTTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTC                                     <  1:128573/149‑1                                                                                      AATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAGCTTCGAAC                                 >  1:123902/1‑149                                                                                        TCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTG                               >  1:601673/1‑149                                                                                         CTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGC                              <  1:111414/149‑1                                                                                             CAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGT                          >  2:217263/1‑149                                                                                              AAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTC                         >  1:712743/1‑149                                                                                                   CACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGG                    <  2:805240/149‑1                                                                                                    ACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGA                   >  1:520294/1‑149                                                                                                      AATTCTCAAAATCAAAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGACCTGCTTCCAAGTTCCCATACTTTCTAGAGAATAGCAACTTCGAACTGCAGGTCGACGGATC                 >  2:696773/1‑149                                                                                                             AAAAGCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAA          <  2:769723/149‑1                                                                                                              AAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAAT         >  1:678399/1‑149                                                                                                                    GAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTTGAGCTGCTTCGAAGTTCCTATACTTTCTAGATAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcga   <  2:201893/149‑7                                                                                                                     AAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgac  >  1:127380/1‑142                                                                                                                                  ||||||||||||||||||||||||||||||||||||||||||||||                                                                                           TAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/4233629‑4233757 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCG  >  NC_000913_3_KO_pgi/4233758‑4233847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.