Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	668,742	C→G	E99Q (GAA→CAA)	rsfS ←	ribosomal silencing factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	668,742	0	C	G	100.0%	104.4 / NA	31	E99Q (GAA→CAA)	rsfS	ribosomal silencing factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (13/18);  total (13/18)

TCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACG  >  NC_000913_3_KO_pgi/668615‑668875                                                                                                                                |                                                                                                                                      tCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTc                                                                                                                  <  2:102594/149‑1 (MQ=255)       ggCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCAt                                                                                                            >  1:480931/1‑149 (MQ=255)         cATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGa                                                                                                          <  1:51975/149‑1 (MQ=255)           tatCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACa                                                                                                        <  1:488756/149‑1 (MQ=255)           tatCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACa                                                                                                        >  1:275558/1‑149 (MQ=255)            atCTATCGGAAAACGACGCAGTTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCAAGGCGACAAGTTGCAGCTTCACGCATTAACTTCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACAt                                                                                                       <  2:221848/149‑1 (MQ=255)               tttCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGa                                                                                                    >  1:182582/1‑149 (MQ=255)                    gAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATc                                                                                               <  2:480931/149‑1 (MQ=255)                          gacgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACCGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCg                                                                                         >  2:287893/1‑149 (MQ=255)                                    ctaggTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCAAGGCGACAAGTTGCAGCTTCACGCATCAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCa                                                                               >  1:97999/4‑149 (MQ=255)                                       gctAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGAATAACATGGACAATCACATCGCCCAAATCCACGa                                                                            <  1:156993/147‑1 (MQ=255)                                           aaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCa                                                                               >  1:262362/1‑142 (MQ=255)                                           aaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCa                                                                               <  2:262362/142‑1 (MQ=255)                                                 gTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCCCGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCCCGGCAATCCAGTc                                                                  >  2:13050/1‑149 (MQ=255)                                                                 gCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCCCCt                                                  >  1:470091/1‑149 (MQ=255)                                                                       tCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACg                                            <  2:31969/149‑1 (MQ=255)                                                                             ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGCACAATCACATCGCCGAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGc                                      <  1:562180/149‑1 (MQ=255)                                                                               aCGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACAt                                                                                           <  1:39546/94‑1 (MQ=255)                                                                               aCGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACAt                                                                                           >  2:39546/1‑94 (MQ=255)                                                                               aCGGCGACAAGTTGCAACTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACACCGCCCAAATCCACGCCAATCCAGTCGGCGCTGCTTTCACCTCCTCCGCCGAGCgg                                    >  1:557788/1‑149 (MQ=255)                                                                                cGGCGACAAGTTGCATCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGt                                   <  1:82604/149‑1 (MQ=255)                                                                                cGGCGACAAGTTGCAGCTTCACGCATCAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGt                                   <  1:142840/149‑1 (MQ=255)                                                                                 ggCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCCCCTTCTACGCCCACCGGTa                                  >  2:254904/1‑149 (MQ=255)                                                                                  gCGAGAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTaa                                 <  1:195864/149‑1 (MQ=255)                                                                                  gCGACAAGTTGCAGCTTCACGCATCAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTaa                                 <  2:97999/149‑1 (MQ=255)                                                                                            gCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCt                       <  1:45568/149‑1 (MQ=255)                                                                                                       cATCAACTCCAGAGTTGTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTc            <  1:14114/149‑1 (MQ=255)                                                                                                            aCTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGca       <  2:275558/149‑1 (MQ=255)                                                                                                                 agagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTTCGCCAGACTCCTGCACAACg  >  2:394098/1‑149 (MQ=255)                                                                                                                  gagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGttt                                                        <  2:249158/94‑1 (MQ=255)                                                                                                                  gagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGttt                                                        >  1:249158/1‑94 (MQ=255)                                                                                                                                |                                                                                                                                      TCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACG  >  NC_000913_3_KO_pgi/668615‑668875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑