Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	4,133,781	C→T	intergenic (+275/‑54)	metF → / → katG	5,10‑methylenetetrahydrofolate reductase/catalase‑peroxidase HPI, heme b‑containing

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	4,133,781	0	C	T	100.0%	332.7 / NA	90	intergenic (+275/‑54)	metF/katG	5,10‑methylenetetrahydrofolate reductase/catalase‑peroxidase HPI, heme b‑containing
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/55);  total (35/55)

GAAGCTAAAACCCTTTTTTATAAAGCATTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAACTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAGGGCGGTCACGACCAGAGTGCGGGGGCGGGCA  >  NC_000913_3_KO_pgi/4133636‑4133925                                                                                                                                                  |                                                                                                                                                 gAAGCTAAAACCCTTTTTTATGAAGCATTTGTCTTGATGCATACATAATAAAACAAGCTTAATTAAGATCAATTTGTTCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTtct                                                                                                                                              <  2:755644/150‑1 (MQ=255) gAAGCTAAAACCCTTTTTTATAAAGCATTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTtct                                                                                                                                              >  3:485989/1‑150 (MQ=255) gAAGCTAAAACCCTTTTTTATAAAGCATTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTtct                                                                                                                                              <  1:721531/150‑1 (MQ=255)   aGCTAAAACCCTTTTTTATAAAGCATTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTtctct                                                                                                                                            <  2:767386/150‑1 (MQ=255)        aaaCCCTTTTTTATAAAGCATTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAAc                                                                                                                                       >  3:524054/1‑150 (MQ=255)            acatttctATAAAACATTTGACCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTAAACCAGCAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCtg                                                                                                                                   <  4:302547/147‑1 (MQ=255)             gttttttATAAAGCATTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCtgt                                                                                                                                  <  4:263929/149‑1 (MQ=255)             cTTTTTTATTAAGCATTTGTCCTTATTCGGACATATTCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCtgt                                                                                                                                  <  4:264004/150‑1 (MQ=255)              ttttttATAAAGCATTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATAGGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCtgtg                                                                                                                                 <  2:769716/150‑1 (MQ=255)                  ttATAAAGCATTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATc                                                                                                                             <  4:360344/150‑1 (MQ=255)                   tataAAGCATTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCg                                                                                                                            <  2:603524/150‑1 (MQ=255)                       aaGCATTTGTCCGAATGCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAGCAATATGTAAGATCTCAACTAGCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAAc                                                                                                                        <  4:498924/150‑1 (MQ=255)                        aGCATTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACg                                                                                                                       >  1:160704/1‑150 (MQ=255)                        aGCATTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTACCGTAACg                                                                                                                       <  2:921034/150‑1 (MQ=255)                         gattatGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTCTCGTAACgg                                                                                                                      >  2:254014/6‑150 (MQ=255)                          cATTTGTCCGAATTCGGACATAATCAAAAATGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGt                                                                                                                     <  1:595044/150‑1 (MQ=255)                          cATTTGTCCGAATTCGGACATAATCAAAAACGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGt                                                                                                                     <  1:595314/150‑1 (MQ=255)                           aTTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTa                                                                                                                    <  1:714584/150‑1 (MQ=255)                            tttGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTaa                                                                                                                   <  1:918821/150‑1 (MQ=255)                                 ccGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTg                                                                                                              <  2:263245/150‑1 (MQ=255)                                 ccGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTg                                                                                                              <  2:718787/150‑1 (MQ=255)                                  cGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGt                                                                                                             >  2:926467/1‑150 (MQ=255)                                  cGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGt                                                                                                             >  2:558294/1‑150 (MQ=255)                                  cGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGt                                                                                                             <  2:561527/150‑1 (MQ=255)                                  cGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGGGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGt                                                                                                             >  3:425150/1‑150 (MQ=255)                                   gAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTa                                                                                                            <  4:530386/150‑1 (MQ=255)                                    aaTTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTag                                                                                                           <  2:593146/150‑1 (MQ=255)                                     aTTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTaga                                                                                                          <  1:359235/150‑1 (MQ=255)                                      ttCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTagag                                                                                                         <  4:485989/150‑1 (MQ=255)                                        cGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAggg                                                                                                       <  3:205853/150‑1 (MQ=255)                                        cGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAggg                                                                                                       <  2:734328/150‑1 (MQ=255)                                          gACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGa                                                                                                     >  1:653668/1‑150 (MQ=255)                                           aCATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAg                                                                                                    <  1:539935/150‑1 (MQ=255)                                            cATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGc                                                                                                   <  2:526006/150‑1 (MQ=255)                                              tAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGcac                                                                                                 >  4:451055/1‑150 (MQ=255)                                                aTCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACAt                                                                                               <  3:176282/150‑1 (MQ=255)                                                 tCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACAtt                                                                                              >  4:297764/1‑150 (MQ=255)                                                 tCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACAtt                                                                                              <  1:96901/150‑1 (MQ=255)                                                    aaaaaGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATtgat                                                                                           <  1:63274/150‑1 (MQ=255)                                                    aaaaaGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGAGGAGCACATtgat                                                                                           >  2:808422/1‑150 (MQ=255)                                                         gCTTAATTAACATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCa                                                                                      <  4:535936/150‑1 (MQ=255)                                                           ttaattaaGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACg                                                                                    >  4:96666/1‑150 (MQ=255)                                                            taattaaGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGt                                                                                   <  1:435527/150‑1 (MQ=255)                                                             aattaaGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTc                                                                                  <  4:575220/150‑1 (MQ=255)                                                                taaGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGa                                                                               <  2:160704/150‑1 (MQ=255)                                                                 aaGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTCACGGTAACACGTTAGAGGGGAGCACATTGATGAGCACGTCAGAc                                                                              <  2:41745/150‑1 (MQ=255)                                                                 aaGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGAc                                                                              <  3:451055/150‑1 (MQ=255)                                                                 aaGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGAc                                                                              <  1:83091/150‑1 (MQ=255)                                                                     tCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACAGGAGAGAGGAGCACATTGATGAGCACGTCAGACGata                                                                          >  2:262361/1‑150 (MQ=255)                                                                        aTTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATcc                                                                       <  1:748434/150‑1 (MQ=255)                                                                                 aCATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAAcacca                                                              <  1:780690/150‑1 (MQ=255)                                                                                   aTCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATtgatg                                                                                          >  1:505894/1‑120 (MQ=255)                                                                                   aTCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATtgatg                                                                                          <  2:505894/120‑1 (MQ=255)                                                                                     ctctTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAacac                                                                >  2:157524/1‑144 (MQ=255)                                                                                     ctctTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAacac                                                                <  1:157524/144‑1 (MQ=255)                                                                                     ctctTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGc                                                          >  1:787928/1‑150 (MQ=255)                                                                                     ctctTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGc                                                          <  2:905786/150‑1 (MQ=255)                                                                                        tttAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCTCATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCAc                                                       >  4:549463/1‑150 (MQ=255)                                                                                        tttAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCa                                                                      >  2:16310/1‑135 (MQ=255)                                                                                        tttAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCa                                                                      <  1:16310/135‑1 (MQ=255)                                                                                           aaCCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTgg                                                    <  1:546813/150‑1 (MQ=255)                                                                                            aCCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGc                                                   >  1:831528/1‑150 (MQ=255)                                                                                               aacaaTATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCaaa                                                >  2:412553/1‑150 (MQ=255)                                                                                               aacaaTATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCaaa                                                >  2:778790/1‑150 (MQ=255)                                                                                               aacaaTATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTCGCgaa                                                >  1:32168/1‑150 (MQ=255)                                                                                               aacaaTATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTCGCaaa                                                >  1:205558/1‑150 (MQ=255)                                                                                                    tatGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGccc                                           <  4:458554/150‑1 (MQ=255)                                                                                                      tGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTa                                                                                                            <  1:430644/83‑1 (MQ=255)                                                                                                      tGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTa                                                                                                            >  2:430644/1‑83 (MQ=255)                                                                                                       gTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGtt                                        >  2:280175/1‑150 (MQ=255)                                                                                                           gATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTagag                                                                                                         >  1:607547/1‑81 (MQ=255)                                                                                                           gATCTCAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTagag                                                                                                         <  2:607547/81‑1 (MQ=255)                                                                                                             tctcAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCa                                  <  1:778790/150‑1 (MQ=255)                                                                                                                cAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAggg                               >  2:176636/1‑150 (MQ=255)                                                                                                                cAACTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAggg                               <  1:926467/150‑1 (MQ=255)                                                                                                                 aaCTATCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAGGGc                              <  2:370123/150‑1 (MQ=255)                                                                                                                      tCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCCGCGCGGTcc                         >  1:436371/1‑149 (MQ=255)                                                                                                                      tCGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAGGGCGGTCa                         >  4:60582/1‑150 (MQ=255)                                                                                                                       cGCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAGGGCGGTCAc                        <  1:176636/150‑1 (MQ=255)                                                                                                                        gCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAGGGCGGTCACg                       >  2:532403/1‑150 (MQ=255)                                                                                                                        gCATCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAGGGCGGTCACg                       >  2:718443/1‑150 (MQ=255)                                                                                                                          aTCCGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCCGGGCGGTCACGAc                     >  3:513719/1‑150 (MQ=255)                                                                                                                            ccGTGGATTAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAGGGCGGTCACGACCa                   <  1:791051/150‑1 (MQ=255)                                                                                                                                    tAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAggg                                                                                                       >  4:189291/1‑58 (MQ=255)                                                                                                                                    tAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAggg                                                                                                       <  3:189291/58‑1 (MQ=255)                                                                                                                                    tAATTCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAGGGCGGTCACGACCAGAGTGCgg           <  1:532403/150‑1 (MQ=255)                                                                                                                                       ttCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGatat                                                                         <  3:48683/85‑1 (MQ=255)                                                                                                                                       ttCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGatat                                                                         >  4:48683/1‑85 (MQ=255)                                                                                                                                       ttCAATTATAATTTCTCTCTAACGCTGTGTATCGTAACGCTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAGGGCGGTCACGACCAGAGTGCggggg        >  2:786286/1‑150 (MQ=255)                                                                                                                                             tataATTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAGGGCGGTCACGACCAGAGTGCGGGGGCGGGca  <  2:170749/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 GAAGCTAAAACCCTTTTTTATAAAGCATTTGTCCGAATTCGGACATAATCAAAAAAGCTTAATTAAGATCAATTTGATCTACATCTCTTTAACCAACAATATGTAAGATCTCAACTATCGCATCCGTGGATTAATTCAATTATAACTTCTCTCTAACGCTGTGTATCGTAACGGTAACACTGTAGAGGGGAGCACATTGATGAGCACGTCAGACGATATCCATAACACCACAGCCACTGGCAAATGCCCGTTCCATCAGGGCGGTCACGACCAGAGTGCGGGGGCGGGCA  >  NC_000913_3_KO_pgi/4133636‑4133925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑