BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	3,552,719	G→T	intergenic (‑246/‑366)	malP ← / → malT	maltodextrin phosphorylase/mal regulon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	3,552,719	0	G	T	100.0%	608.8 / NA	165	intergenic (‑246/‑366)	malP/malT	maltodextrin phosphorylase/mal regulon transcriptional activator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (88/77); total (88/77)

Only 100 of 165 total aligned reads displayed.

GTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAAGGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAAT  >  NC_000913_3_KO_pgi/3552574‑3552864

gTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTaa                                                                                                                                               <  1:981231/150‑1 (MQ=255)

  tGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAAct                                                                                                                                             <  1:378910/150‑1 (MQ=255)

   gCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAActc                                                                                                                                            >  1:1573220/1‑150 (MQ=255)

    cGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCACAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTAGAAAAAAAAAAAAAAGCGGAATGTAActct                                                                                                                                           <  2:1235663/150‑1 (MQ=255)

       ggATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCtata                                                                                                                                        >  2:440965/1‑150 (MQ=255)

        gaggAGGAAGGAAATGTGATGTCAAACACTTAAAGATAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGAAAAAAAAAAAAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATaa                                                                                                                                       <  4:546205/147‑1 (MQ=255)

          tGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAAt                                                                                                                                     >  1:1500551/1‑150 (MQ=255)

             gCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTaa                                                                                                                                  >  1:859564/1‑150 (MQ=255)

             gCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTaa                                                                                                                                  >  4:757513/1‑150 (MQ=255)

             gCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCAGTATCTAAGGGCAAATAATGGCCGTGGCGTATTTTCAAAGAGCGGAATGTAACTCTATAAATTaa                                                                                                                                  <  2:1275229/150‑1 (MQ=255)

             gCAAGGAAATGTGATCTCAAACAATAAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATCaa                                                                                                                                  <  2:1005909/150‑1 (MQ=255)

              cAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATCAAATAAg                                                                                                                                 >  4:957052/1‑150 (MQ=255)

               aaGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGt                                                                                                                                >  2:340529/1‑150 (MQ=255)

               aaGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGt                                                                                                                                >  1:772307/1‑150 (MQ=255)

                     aTGTGATCTCAACCACTTAAATCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGa                                                                                                                          <  1:440965/150‑1 (MQ=255)

                     aTGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGa                                                                                                                          >  4:932120/1‑150 (MQ=255)

                          aTCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCCAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCAGAATGTAACTCTATAAATTAAGTAAAGGAGTGaa                                                                                                                     >  1:1231411/1‑150 (MQ=255)

                             tcAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGt                                                                                                                                <  2:395241/136‑1 (MQ=255)

                             tcAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACa                                                                                                                  >  1:1280260/1‑150 (MQ=255)

                                aCCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGtt                                                                                                               <  1:1093847/150‑1 (MQ=255)

                                aCCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGtt                                                                                                               <  1:1134785/150‑1 (MQ=255)

                                 ccACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGttt                                                                                                              <  3:27591/150‑1 (MQ=255)

                                  cACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTaa                                                                                                                                               <  2:1026888/116‑1 (MQ=255)

                                  cACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTaa                                                                                                                                               >  1:1026888/1‑116 (MQ=255)

                                   aCTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCa                                                                                                            >  4:743134/1‑150 (MQ=255)

                                    cTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCAt                                                                                                           >  3:741321/1‑150 (MQ=255)

                                    cTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCAt                                                                                                           >  1:1374595/1‑150 (MQ=255)

                                      tAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTGTGTAAAGGAGTGAAACAGTTTCATaa                                                                                                         <  4:552703/150‑1 (MQ=255)

                                      tAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATaa                                                                                                         <  1:151548/150‑1 (MQ=255)

                                       aaaGCTAGTGCAAACCACCGGATTAGCATCAAATCAATGCAATACAGTGCAGAAAATCAGTATCTAAGTGCAAAAACTCGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAg                                                                                                        >  2:1040016/1‑150 (MQ=255)

                                       aaaGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGt                                                                                                                        <  2:599413/134‑1 (MQ=255)

                                       aaaGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAg                                                                                                        >  4:29244/1‑150 (MQ=255)

                                       aaaGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAg                                                                                                        <  1:809993/150‑1 (MQ=255)

                                          gCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTaa                                                                                                     >  4:635194/1‑150 (MQ=255)

                                               tGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAatat                                                                                                >  1:388937/1‑150 (MQ=255)

                                                gCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATc                                                                                               <  2:1086718/150‑1 (MQ=255)

                                                 cAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATcc                                                                                              >  2:951843/1‑150 (MQ=255)

                                                  aaaCCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAAt                                                                                                                                     >  2:92018/1‑110 (MQ=255)

                                                  aaaCCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAAt                                                                                                                                     <  1:92018/110‑1 (MQ=255)

                                                  aaaCCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTGAAGGAGTGAAACAGTTTCATAAGTAAAATATCCa                                                                                             >  1:1230444/1‑150 (MQ=255)

                                                  aaaCCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCa                                                                                             >  2:496775/1‑150 (MQ=255)

                                                  aaaCCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCa                                                                                             >  1:1630018/1‑150 (MQ=255)

                                                   aaCCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGGGTGAAACAGTTTCATAAGTAAAATATCCAg                                                                                            <  2:1124209/150‑1 (MQ=255)

                                                      cacaGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAgtgt                                                                                         >  2:1656705/1‑150 (MQ=255)

                                                      cacaGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAgtgt                                                                                         >  2:1549446/1‑150 (MQ=255)

                                                         aGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCt                                                                                      >  2:917981/1‑150 (MQ=255)

                                                          ggATTAGCAACAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTc                                                                                     <  3:425467/150‑1 (MQ=255)

                                                           gATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTcc                                                                                    >  2:1461122/1‑150 (MQ=255)

                                                              tAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCAtc                                                                                 <  2:1573220/150‑1 (MQ=255)

                                                                  atcaaatcaaTGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAAc                                                                                                                   >  2:1549751/1‑112 (MQ=255)

                                                                  atcaaatcaaTGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCAt                                                                             >  1:639738/1‑150 (MQ=255)

                                                                  atcaaatcaaTGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCAt                                                                             >  2:694056/1‑150 (MQ=255)

                                                                   tcaaatcaaTGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATACGTAAAATATCCAGTGTGCTCCATCTCAtt                                                                            >  2:1061959/1‑150 (MQ=255)

                                                                   tcaaatcaaTGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCAtt                                                                            >  2:1626825/1‑150 (MQ=255)

                                                                   tcaaatcaaTGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCAtt                                                                            >  3:716592/1‑150 (MQ=255)

                                                                   tcaaatcaaTGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCAtt                                                                            >  3:532880/1‑150 (MQ=255)

                                                                        tcaaTGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTaa                                                                       <  2:1630018/150‑1 (MQ=255)

                                                                          aaTGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGt                                                                                                                                >  1:436154/1‑91 (MQ=255)

                                                                          aaTGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATa                                                                     >  4:442127/1‑150 (MQ=255)

                                                                             gCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGAt                                                                  <  1:397121/150‑1 (MQ=255)

                                                                                      cgcAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGa                                                         >  2:521119/1‑150 (MQ=255)

                                                                                       gcAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTc                                                                                                             >  4:717162/1‑97 (MQ=255)

                                                                                         aGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGtt                                                                                                               >  2:999889/1‑93 (MQ=255)

                                                                                         aGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGatca                                                      >  1:294824/1‑150 (MQ=255)

                                                                                             aaTCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCtt                                                  <  3:820101/150‑1 (MQ=255)

                                                                                               tCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCtttt                                                <  1:1549446/150‑1 (MQ=255)

                                                                                                cTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCttttt                                               >  2:841376/1‑150 (MQ=255)

                                                                                                atGTATCTAAGTTCAAAAAATGGACGTTGCGTATTTTCAAAAAGCGGAATGTAAATCTATAAATTAAGTAAAGGAGTGAAACAGTTTCAAAAGTAAAATAACAAGAGTGCAACATCTCATTCTTCAAAGTTTTATAAAAATCATAttttt                                               >  2:581244/2‑150 (MQ=255)

                                                                                                  gTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGCGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAg                                             >  2:664913/1‑150 (MQ=255)

                                                                                                   tATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGa                                            <  2:834317/150‑1 (MQ=255)

                                                                                                    aTCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGAt                                           <  2:691613/150‑1 (MQ=255)

                                                                                                    aTCTAAGTGAAAAAAATGGCCGTAACGTAATTTAAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGAt                                           <  2:1394052/150‑1 (MQ=255)

                                                                                                      cTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATgg                                         >  2:910710/1‑150 (MQ=255)

                                                                                                      cTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATgg                                         >  4:874798/1‑150 (MQ=255)

                                                                                                          gTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATGAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAg                                                                    >  1:629837/1‑119 (MQ=255)

                                                                                                          gTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACt                                     <  3:598385/150‑1 (MQ=255)

                                                                                                             cAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGa                                                         >  2:375382/1‑127 (MQ=255)

                                                                                                              aaaaaaTGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTtca                                 >  4:470994/1‑150 (MQ=255)

                                                                                                              aaaaaaTGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAAATCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTtca                                 >  2:1488020/1‑150 (MQ=255)

                                                                                                               aaaaaTGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTtcat                                >  3:633153/1‑150 (MQ=255)

                                                                                                                aaaaTGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTtcatc                               <  2:1374595/150‑1 (MQ=255)

                                                                                                                 aaaTGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTATGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATTGCACTTtcatcc                              >  2:1302530/1‑149 (MQ=255)

                                                                                                                   aTGGCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAgg                            >  2:219557/1‑150 (MQ=255)

                                                                                                                     ggCCGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGaa                          <  1:932359/150‑1 (MQ=255)

                                                                                                                       ccGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATg                        <  3:464936/150‑1 (MQ=255)

                                                                                                                       ccGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATg                        <  3:743134/150‑1 (MQ=255)

                                                                                                                       ccGTTGCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATg                        <  3:943815/150‑1 (MQ=255)

                                                                                                                            gCGTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATgaagaa                   <  3:986921/150‑1 (MQ=255)

                                                                                                                              gTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCAt                                                                                                           <  3:192163/60‑1 (MQ=255)

                                                                                                                              gTATTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCAt                                                                                                           >  4:192163/1‑60 (MQ=255)

                                                                                                                                aTTTTCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGaaa               <  1:1040016/150‑1 (MQ=255)

                                                                                                                                  tttCAAAAAGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAAcc             <  3:874798/150‑1 (MQ=255)

                                                                                                                                      aaaaaGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTg         >  1:198678/1‑150 (MQ=255)

                                                                                                                                      aaaaaGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTg         <  1:1366398/150‑1 (MQ=255)

                                                                                                                                       aaaaGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATttat                                                              >  2:246485/1‑96 (MQ=255)

                                                                                                                                       aaaaGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGc        >  2:1541016/1‑150 (MQ=255)

                                                                                                                                        aaaGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCt       >  2:540225/1‑150 (MQ=255)

                                                                                                                                         aaGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCtt      <  1:1061959/150‑1 (MQ=255)

                                                                                                                                          aGCGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAAGAGGTTCAGAATTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTa     >  4:834165/1‑150 (MQ=255)

                                                                                                                                            cGGAATGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTaaa   >  2:1296777/1‑150 (MQ=255)

GTTGCGGGGATGAGCAAGGAAATGTGATCTCAACCACTTAAAGCTAGTGCAAACCACAGGATTAGCATCAAATCAATGCAATACAGCGCAGAAAATCTGTATCTAAGTGCAAAAAATGGCCGTTGCGTATTTTCAAAAAGCGGAAGGTAACTCTATAAATTAAGTAAAGGAGTGAAACAGTTTCATAAGTAAAATATCCAGTGTGCTCCATCTCATTCTTAATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAAT  >  NC_000913_3_KO_pgi/3552574‑3552864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`