BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_pgi	4,235,435	Δ1 bp	intergenic (‑2202/‑499)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_pgi	4,235,433	0	A	.	100.0%	1307.9 / NA	287	intergenic (‑2200/‑501)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (145/142); total (145/142)

Only 100 of 306 total aligned reads displayed.

CTTTGACCAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATAAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTG  >  NC_000913_3_hsa_pgi/4235284‑4235578

cTTTGACCAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATa                                                                                                                                                   >  2:1670097/1‑150 (MQ=255)

 tttGACCAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCGAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATaa                                                                                                                                                  >  1:2916334/1‑150 (MQ=255)

  ttGACCAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATaat                                                                                                                                                 <  2:3962869/150‑2 (MQ=255)

  ttGACCAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATaat                                                                                                                                                 <  1:1924169/150‑2 (MQ=255)

    gACCAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTTACCTCTCACGACGCTTCTACGAATGGGCTGAGCAACTTCATCAAGCAGCAGAGCGAGGCCAGAGTCCAATaatca                                                                                                                                               >  2:189082/1‑147 (MQ=255)

    gACCAGTGGGGAGTGGAGCGGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATaatca                                                                                                                                               <  2:3566465/150‑4 (MQ=255)

      ccAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGCCGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑Aatcatc                                                                                                                                            >  2:2828171/1‑150 (MQ=255)

      ccAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑Aatcatc                                                                                                                                            >  2:3699206/1‑150 (MQ=255)

              gAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGatat                                                                                                                                    <  1:1457917/150‑1 (MQ=255)

               aGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATg                                                                                                                                   <  1:55929/150‑1 (MQ=255)

                gTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGt                                                                                                                                  <  2:3818764/150‑1 (MQ=255)

                  ggAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAg                                                                                                                                >  1:1585087/1‑150 (MQ=255)

                  ggAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAg                                                                                                                                >  2:3180443/1‑150 (MQ=255)

                   gAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAgg                                                                                                                               >  1:2584992/1‑150 (MQ=255)

                   gAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAgg                                                                                                                               >  1:3360624/1‑150 (MQ=255)

                      cTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCg                                                                                                                            >  2:554930/1‑150 (MQ=255)

                       tGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCGTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCgg                                                                                                                           >  2:540748/1‑150 (MQ=255)

                        gggAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTATGCCGGa                                                                                                                          >  1:3552542/1‑150 (MQ=255)

                         ggAAAGCATCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑ATTCATCGTCGATATGTAGGCCGGAt                                                                                                                         <  2:2256914/150‑1 (MQ=255)

                            aaGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                      <  1:4093102/150‑1 (MQ=255)

                              gcagcTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                                    >  1:1645422/1‑150 (MQ=255)

                                agcTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGt                                                                                                                  >  2:1214244/1‑150 (MQ=255)

                                  cTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTAAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTc                                                                                                                <  2:2844631/150‑1 (MQ=255)

                                      cTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                            <  1:3257561/150‑1 (MQ=255)

                                      cTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                            >  2:1381708/1‑150 (MQ=255)

                                       tAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCAGCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                           <  1:604117/150‑1 (MQ=255)

                                        aaGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgccg                                                                                                          >  2:1176065/1‑150 (MQ=255)

                                         aGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgccgc                                                                                                         >  1:3241753/1‑150 (MQ=255)

                                         aGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACATCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAccccgc                                                                                                         >  1:481474/1‑150 (MQ=255)

                                             aaTAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                     <  2:1309086/150‑1 (MQ=255)

                                               tAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                   <  1:3183492/150‑1 (MQ=255)

                                               aagagCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                   <  1:3840319/149‑1 (MQ=255)

                                                      tGAGCTTGATGGCAGTGATCATGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGTGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGa                                                                                            <  2:3914337/150‑1 (MQ=255)

                                                        aGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATg                                                                                          <  2:3982208/150‑1 (MQ=255)

                                                           ttGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCt                                                                                       >  1:940388/1‑150 (MQ=255)

                                                            tGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTg                                                                                      <  1:2229081/150‑1 (MQ=255)

                                                            tGATCGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTg                                                                                      >  1:2418343/1‑150 (MQ=255)

                                                               tGGCAGTGCTCAAGTGACCTCTCTCGACGCTTCTACCAATGGGCTCATCAACTTCTTCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATg                                                                                   <  1:2651304/150‑1 (MQ=255)

                                                               tGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATg                                                                                   <  1:359767/150‑1 (MQ=255)

                                                                     tGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATTATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgc                                                                             >  2:778252/1‑150 (MQ=255)

                                                                      gCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgcg                                                                            <  2:4136683/150‑1 (MQ=255)

                                                                       cTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCgg                                                                           <  1:1773172/150‑1 (MQ=255)

                                                                         cAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGCTGCCTGATGCGACGCGGTc                                                                         >  2:2099905/1‑150 (MQ=255)

                                                                         cAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACCCCGCATCCGGCAACCGATGCCAGATGCGAccacctc                                                                         >  1:719169/1‑144 (MQ=39)

                                                                                 ctctCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa                                                                 <  1:3650316/150‑1 (MQ=255)

                                                                                 ctctCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa                                                                 >  1:3012398/1‑150 (MQ=255)

                                                                                  tctcACGACGCTTCTACCAATGGGCTCATCAACTTCATCCAGCAGCAGCGCGAGGCCAGAGACCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTGCACGCCGCAACCGGCAACCGATGCCTGATGCGACGCGGTCGCGGCTTAt                                                                >  1:2617784/1‑150 (MQ=38)

                                                                                    tcACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                              <  2:745938/150‑1 (MQ=255)

                                                                                     cACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                             >  2:1093338/1‑150 (MQ=255)

                                                                                     cACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGGCtttgcag                                                             >  2:4025435/1‑145 (MQ=255)

                                                                                     cACGACGCTTCTACCAAAGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGGTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                             <  1:3796218/150‑1 (MQ=255)

                                                                                        gacgCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc                                                          <  1:1954595/150‑1 (MQ=255)

                                                                                        gacgCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc                                                          <  2:3452544/150‑1 (MQ=255)

                                                                                         acgCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGTGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCt                                                         <  2:1991800/150‑1 (MQ=255)

                                                                                           gCTTCAACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGACCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAGGcatcg                                                       <  1:1862616/150‑5 (MQ=37)

                                                                                            cTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACa                                                      <  2:3256221/150‑1 (MQ=255)

                                                                                             ttCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg                                                     >  2:3073374/1‑150 (MQ=255)

                                                                                             ttCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg                                                     >  2:2887439/1‑150 (MQ=255)

                                                                                              tCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAgg                                                    <  1:3699206/150‑1 (MQ=255)

                                                                                                tcccAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCCTCGTCGATATGTAGGCCGGTTAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                                  <  2:3748252/148‑1 (MQ=255)

                                                                                                tACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                                  <  1:428091/150‑1 (MQ=255)

                                                                                                tACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                                  <  2:467183/150‑1 (MQ=255)

                                                                                                  ccAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGa                                                <  1:2123232/150‑1 (MQ=255)

                                                                                                   cAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGCTGCCTGAAGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGAt                                               >  1:3967090/1‑150 (MQ=255)

                                                                                                      tGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                            <  1:297521/150‑1 (MQ=255)

                                                                                                       gggCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCg                                           <  1:4009308/150‑1 (MQ=255)

                                                                                                        ggCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGCTGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                                          >  1:2028732/1‑150 (MQ=255)

                                                                                                        ggCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                                          >  1:3040360/1‑150 (MQ=255)

                                                                                                          cTCAGCACCTTCATCAAGCAGCAGCGCGAGGCCAGAGCCCAAT‑AATCATCGGCGATATGTAGGCCGGATAAGGCGATCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                        <  1:839654/150‑1 (MQ=25)

                                                                                                           tcatcaACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat                                       <  1:1176065/150‑1 (MQ=255)

                                                                                                            catcaACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg                                      >  1:2847340/1‑150 (MQ=255)

                                                                                                              tcaACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTa                                    >  1:1845562/1‑150 (MQ=255)

                                                                                                                 aCTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAcc                                                                                              >  2:3837565/1‑89 (MQ=255)

                                                                                                                  cTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                                >  2:2462954/1‑150 (MQ=255)

                                                                                                                   ttCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGTTATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg                               <  2:1692524/150‑1 (MQ=255)

                                                                                                                   ttCATCAAGCAGCAGCACGAGGCCAGAGGCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg                               <  2:2588391/150‑1 (MQ=255)

                                                                                                                      atcaAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGCTGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAt                            >  1:282088/1‑150 (MQ=255)

                                                                                                                         aaGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCg                         <  2:3000901/150‑1 (MQ=255)

                                                                                                                         aaGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGAGTACACGCCCCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATGAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCg                         >  1:3908517/1‑150 (MQ=255)

                                                                                                                          agcagcagcGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg                        <  1:1299660/150‑1 (MQ=255)

                                                                                                                          agcagcagcGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg                        <  2:3581068/150‑1 (MQ=255)

                                                                                                                           gcagcagcGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                       <  1:3386406/150‑1 (MQ=255)

                                                                                                                           gcagcagcGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                       <  2:4055017/150‑1 (MQ=255)

                                                                                                                            cagcagcGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCa                      >  2:2684727/1‑150 (MQ=255)

                                                                                                                            cagcagcGCGAGGCCAGAGGCCAAT‑AATCATCGTCGATCTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCGACAGGTCGATGCCGATATGTACATCGTATTCGGCa                      <  1:2547017/150‑1 (MQ=255)

                                                                                                                              gcagcGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAt                    >  1:2166064/1‑150 (MQ=255)

                                                                                                                                agcGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTa                  >  1:101812/1‑150 (MQ=255)

                                                                                                                                   gcgAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa               <  2:3394470/150‑1 (MQ=255)

                                                                                                                                   gcgAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGGCTTATCAGGCCGACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa               >  1:3492799/1‑150 (MQ=255)

                                                                                                                                        gCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAg          >  2:3041152/1‑150 (MQ=255)

                                                                                                                                         ccAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGc         <  2:3324477/150‑1 (MQ=255)

                                                                                                                                          cAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGTTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCa        <  2:85698/150‑1 (MQ=255)

                                                                                                                                          cAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCa        >  2:2241988/1‑150 (MQ=255)

                                                                                                                                           agagTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATAAGCCCAACAAGTCGATGCCGATATGTACATCGTATTCGGCAATTAAAACATAGCAc       >  1:3425376/1‑150 (MQ=37)

                                                                                                                                             agTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCTCga     >  1:2288491/1‑150 (MQ=255)

                                                                                                                                              gTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCTCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgat    <  2:1547975/150‑1 (MQ=255)

                                                                                                                                               tCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt   <  2:3360624/150‑1 (MQ=255)

                                                                                                                                               tCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt   >  2:2179684/1‑150 (MQ=255)

                                                                                                                                                ccAAT‑AATCATCGTCGATATGTAGGCCGGATACGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattg  >  1:170898/1‑150 (MQ=255)

                                                                                                                                                ccAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattg  <  1:1412163/150‑1 (MQ=255)

CTTTGACCAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATAAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTG  >  NC_000913_3_hsa_pgi/4235284‑4235578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`