Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,233,708 C→T intergenic (‑475/‑2199) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,233,7080CT93.3% 277.5 / 7.3 90intergenic (‑475/‑2199)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base T (42/42);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

GGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCG  >  NC_000913_3_bme_pgi/4233566‑4233854
                                                                                                                                              |                                                                                                                                                  
ggTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACaa                                                                                                                                             <  4:87910/150‑1 (MQ=255)
     tCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTc                                                                                                                                        >  2:1063313/1‑150 (MQ=255)
       aaCGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACCGTTGCTTCAAGCTTcgt                                                                                                                                      >  2:403296/1‑148 (MQ=255)
       aaCGATGGGGTCATGCGGATTTTTCATCCACACATGGCGGTCAGTAGTTAAGCTAATAAATTCTTCACTGCGCTAAGGGGTTACACTCAACATTACGCTATCGGCCCTAAAACCATCCCAATTTTCTGTGACTGGTGCTACAATCTTCCa                                                                                                                                      >  2:795583/1‑150 (MQ=255)
             ggggTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTca                                                                                                                                <  2:968632/150‑1 (MQ=255)
              gggTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTcac                                                                                                                               <  2:1198302/150‑1 (MQ=255)
                    tGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATtct                                                                                                                         <  1:265321/150‑1 (MQ=255)
                      cGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGAACTAAAACCGTCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCa                                                                                                                       <  1:250928/150‑1 (MQ=255)
                       ggATTTTTCATCCACTCCTGGCGGACAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCaa                                                                                                                      >  2:164349/1‑150 (MQ=255)
                         aTTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCaaaa                                                                                                                    <  2:638517/150‑1 (MQ=255)
                              tCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAACGTCACAATTCTCAAAATCaga                                                                                                               <  4:32531/150‑1 (MQ=255)
                               cATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCACAGTCACAATTCTCAAAATCagaa                                                                                                              <  1:554302/150‑1 (MQ=255)
                                        cTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCGCAAAATCAGAAGAGTATTGc                                                                                                     <  4:321163/150‑1 (MQ=255)
                                            cGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAAt                                                                                                 <  4:700989/150‑1 (MQ=255)
                                                  gtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaa                                                                                                              >  1:1116455/1‑131 (MQ=255)
                                                  gtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaa                                                                                                              <  2:1116455/131‑1 (MQ=255)
                                                        cAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGAcgc                                                                                     >  4:438727/1‑150 (MQ=255)
                                                          gCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGcga                                                                                   >  3:172354/1‑150 (MQ=255)
                                                            taataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGcgacg                                                                                 >  1:374883/1‑150 (MQ=255)
                                                                  aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTa                                                                                                                                                <  1:778278/81‑1 (MQ=255)
                                                                  aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCtt                                                                           <  1:795583/150‑1 (MQ=255)
                                                                  aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCtt                                                                           >  1:298326/1‑150 (MQ=255)
                                                                  aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCtt                                                                           <  1:557565/150‑1 (MQ=255)
                                                                  aTGCTTCACTGCGCTAAGAGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTa                                                                                                                                                >  2:778278/1‑81 (MQ=255)
                                                                    gCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGa                                                                         >  1:48494/1‑150 (MQ=255)
                                                                     cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGaa                                                                        <  3:162074/150‑1 (MQ=255)
                                                                      ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAg                                                                       >  3:85015/1‑150 (MQ=255)
                                                                      ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAg                                                                       >  1:1249714/1‑150 (MQ=255)
                                                                         aCTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTTTTTCTTGTGCTACAATCTTCCAAAGACACAATTCACAAAATAAGAAGAGGATGACTAATGTCAAGAGACGGAACTAAGCGGGGAGCaa                                                                    >  4:354359/1‑150 (MQ=255)
                                                                              gcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTc                                                               >  2:380010/1‑150 (MQ=255)
                                                                                  aGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACaa                                                           >  3:659249/1‑150 (MQ=255)
                                                                                  aGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACaa                                                           >  4:26070/1‑150 (MQ=255)
                                                                                    ggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCaaa                                                                                                                     >  2:1037372/1‑90 (MQ=255)
                                                                                    ggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCaaa                                                                                                                     <  1:1037372/90‑1 (MQ=255)
                                                                                     gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGc                                                        >  2:1145797/1‑150 (MQ=255)
                                                                                     gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGc                                                        <  2:298326/150‑1 (MQ=255)
                                                                                      tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaa                                                                                                              >  2:979720/1‑95 (MQ=255)
                                                                                      tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaa                                                                                                              <  1:979720/95‑1 (MQ=255)
                                                                                      tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCt                                                       >  3:308809/1‑150 (MQ=255)
                                                                                       ttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTg                                                                                                      >  4:652336/1‑102 (MQ=255)
                                                                                       ttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTg                                                                                                      <  3:652336/102‑1 (MQ=255)
                                                                                         acacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaa                                                    <  2:323703/150‑1 (MQ=255)
                                                                                            cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaaaaa                                                 <  4:308809/150‑1 (MQ=255)
                                                                                             tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATtctc                                                                                                                        >  1:1089917/1‑78 (MQ=255)
                                                                                             tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATtctc                                                                                                                        <  2:1089917/78‑1 (MQ=255)
                                                                                             tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCaa                                                                                                                      <  4:561374/80‑1 (MQ=255)
                                                                                             tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCaa                                                                                                                      >  3:561374/1‑80 (MQ=255)
                                                                                             tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaa                                                    <  1:392418/146‑1 (MQ=255)
                                                                                             tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaa                                                    >  2:392418/1‑146 (MQ=255)
                                                                                             tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAg                                                >  2:638539/1‑150 (MQ=255)
                                                                                             tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAg                                                >  3:298889/1‑150 (MQ=255)
                                                                                               aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTTTGACTGGTTCTACAATCTTCCAAATTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCa                                              >  2:787187/1‑150 (MQ=255)
                                                                                               aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTTTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGt                                                                >  2:498847/1‑132 (MQ=255)
                                                                                               aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTTTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGt                                                                <  1:498847/132‑1 (MQ=255)
                                                                                               aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCTCTACAAt                                                                                                                                            <  4:642211/56‑1 (MQ=255)
                                                                                               aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAAt                                                                                                                                            >  3:642211/1‑56 (MQ=255)
                                                                                               aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCa                                              >  3:560059/1‑150 (MQ=255)
                                                                                                 cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCAtt                                            >  2:539625/1‑150 (MQ=255)
                                                                                                      cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATCCTCaaa                                                                                                                     <  1:323743/72‑1 (MQ=255)
                                                                                                      cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATCCTCaaa                                                                                                                     >  2:323743/1‑72 (MQ=255)
                                                                                                       gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTc                                                               <  1:258028/125‑1 (MQ=255)
                                                                                                       gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTc                                                               >  2:258028/1‑125 (MQ=255)
                                                                                                         tAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAgaga                                                                                        <  3:141641/98‑1 (MQ=255)
                                                                                                         tAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAgaga                                                                                        >  4:141641/1‑98 (MQ=255)
                                                                                                         tAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGaa                                    <  3:26070/150‑1 (MQ=255)
                                                                                                           aCGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATc                                  <  2:294410/150‑1 (MQ=255)
                                                                                                           aCGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATc                                  <  4:172354/150‑1 (MQ=255)
                                                                                                           aCGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATc                                  <  4:560059/150‑1 (MQ=255)
                                                                                                              gCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGc                               >  1:837944/1‑150 (MQ=255)
                                                                                                              gCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGc                               >  2:206020/1‑150 (MQ=255)
                                                                                                                aCTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCa                                                                                                                 >  3:737437/1‑66 (MQ=255)
                                                                                                                aCTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCa                                                                                                                 <  4:737437/66‑1 (MQ=255)
                                                                                                                  tAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCa                                                                                                                 <  2:56269/64‑1 (MQ=255)
                                                                                                                  tAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCa                                                                                                                 >  1:56269/1‑64 (MQ=255)
                                                                                                                  tAAAACCATCACATTTTTCTGTAACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTCAAAAAGCATTGGGCGGGATCGGccccc                           >  2:746082/1‑149 (MQ=255)
                                                                                                                      cccATCACATTTTTCTGTGACTGGTGCTACAATCTGCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgcg                       <  3:354359/149‑1 (MQ=255)
                                                                                                                      aCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAaga                                                                                          <  2:799594/83‑1 (MQ=255)
                                                                                                                      aCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAaga                                                                                          >  1:799594/1‑83 (MQ=255)
                                                                                                                            acaTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgc                 <  3:438727/150‑1 (MQ=255)
                                                                                                                              aTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaa                                                                                                              <  3:239181/55‑1 (MQ=255)
                                                                                                                              aTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaa                                                                                                              >  4:239181/1‑55 (MQ=255)
                                                                                                                              aTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGcga                                                                                   <  1:1211972/82‑1 (MQ=255)
                                                                                                                              aTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGcga                                                                                   >  2:1211972/1‑82 (MQ=255)
                                                                                                                              aTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc               <  1:638539/150‑1 (MQ=255)
                                                                                                                              aTTTTTCTGTGACTGGTGCGACAAGCTTCCAAAGTCACAATTCTCAAAACCAGCAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc               <  1:746082/150‑1 (MQ=255)
                                                                                                                                 tttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGcgcgcg            <  1:380010/150‑1 (MQ=255)
                                                                                                                                     tgtgACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAAt                                                                                                 >  2:714050/1‑61 (MQ=255)
                                                                                                                                     tgtgACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAAt                                                                                                 <  1:714050/61‑1 (MQ=255)
                                                                                                                                     tgtgACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCAt        >  2:140289/1‑150 (MQ=255)
                                                                                                                                           tGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAgcg  <  1:130164/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
GGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCG  >  NC_000913_3_bme_pgi/4233566‑4233854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: