Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913_3_bme_pgi | 4,233,746 | A→G | intergenic (‑513/‑2161) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913_3_bme_pgi | 4,233,746 | 0 | A | G | 97.4% | 121.7 / NA | 38 | intergenic (‑513/‑2161) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (23/14); minor base C (1/0); total (24/14) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGC > NC_000913_3_bme_pgi/4233604‑4233889 | tCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTAt > 1:390599/1‑149 (MQ=255) agtagtTCAGCTAATACATCCTTCCCTGCGCTAAGGCTTCACCCTCACCCTTACGCTAACGGCACTAAAACCAACCCATTTTTCTGTCACTGGCGCTCCAATCTTCCAAAGTCACAATCCTCACAATCACACGAGCCTTCCTaacgccc > 2:445807/1‑144 (MQ=255) acgtaataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGAcgc < 1:154132/146‑1 (MQ=255) aGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGAcgc < 1:574458/149‑1 (MQ=255) aGCTAATAAAGGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGAcgc > 2:997500/1‑149 (MQ=255) tcaaTGCTTCACTGCGGTAAGGGTTTACCCTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGGCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGaa < 1:973956/147‑1 (MQ=255) aaaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAg < 2:742606/149‑1 (MQ=255) aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGc > 2:118426/1‑149 (MQ=255) aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACACGACGAAGCt > 1:292192/1‑149 (MQ=255) cgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAag > 2:985256/1‑123 (MQ=255) cgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAag < 1:985256/123‑1 (MQ=255) ttAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGCCGAAGCTTGAAGCAACGGTc > 2:176996/2‑149 (MQ=255) tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCACAGc > 1:512679/1‑149 (MQ=255) cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAAACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaca > 2:941340/1‑147 (MQ=255) tCAACATTACGCTAACGGCACTAAAACCATCCCATTTTTCTGTGACTGGCGCTCCAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCCACGGTCGCACAGCTGaaaaa > 2:933269/1‑149 (MQ=255) tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAACGCTGaaaaa > 2:936699/1‑149 (MQ=255) aTTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGGGGTTCTTAGCAACGGTCGCAAAGCTGAAAAAGCAtt < 1:997500/149‑1 (MQ=255) tacgtaaaCGGCACTAAAACGCTCACATCTTTTTGTGACTGGCGCTACAATCGTCCAAAGTCAAAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTgg < 2:199207/143‑1 (MQ=255) aCTAAAACCATCACATTTTTCTGTTACTGGCGCTACAATTTTCCAAAGTCACAATTATCAAAATAGGAGTAGGGTTGCTAATGCCCCGAATGTTGTTAAATCGTGTAGCTACGGTCGTAATTCTGAAAAAGCATGGTGCTGAATCGGcc > 2:717954/1‑149 (MQ=255) aCTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc > 1:415734/1‑149 (MQ=255) aaaCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgc > 1:533660/1‑149 (MQ=255) aCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc > 2:517114/1‑149 (MQ=255) aCCATCACATTTTTCTGTGACTGGCGCTACAAGCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc < 1:365393/149‑1 (MQ=255) caTTTTTCTGGGACTGGCGCTACAAACTTCCAAAGCCACAATTCTCAAAATCAGAGGAGTATTGCGAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATgc < 1:189620/149‑1 (MQ=255) tCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGCATAT‑CGCGCgg < 2:226323/149‑1 (MQ=255) cTGTCACTGGCGCTACCATCTTCCAACGTCACAATTCTCAAACTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCGCGCGC‑‑ATAT‑CGCGCggggc < 2:592908/149‑4 (MQ=255) aaTCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGCAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑CTATGCGCGCGGCATTCAGCGCCGAcccc > 1:78198/1‑149 (MQ=255) tCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccgg > 2:630711/1‑149 (MQ=255) tCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccgg > 1:561560/1‑149 (MQ=255) tCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccgg > 1:336290/1‑149 (MQ=255) ttCCAAAGTCACAATTCTCAAAAACAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccggcc < 1:118426/149‑1 (MQ=255) tCCAAATTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACTGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccggccg < 1:81513/149‑1 (MQ=255) aaaGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGATAACGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCCTTCAGCGCCGACCACGCCCGGtc > 2:940090/1‑148 (MQ=255) acaATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCg > 1:405721/1‑149 (MQ=255) tcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGCCGAAGCTTGCAGCAACGGTCGAAAAGATGCAAAACCATTGGGACGACTCCACCAGGCCCG‑ATATCGCCTCGCCATGGAGGGCCGACCCCCGCCGGTTCTGGCGATAttgc > 2:593932/1‑147 (MQ=255) tcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCg > 2:347624/1‑149 (MQ=255) tCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCGCTGc > 1:433643/1‑149 (MQ=255) ccAGAGGAGTATTGCTAATGGAAAGAGACGTGACGAATCTATAAGAACCGGTCGCAAATCTGAAAAAGCCTTGGGCGGAATCGGCCCTGCGCG‑TTATCCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc < 1:332068/148‑1 (MQ=255) | TCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGC > NC_000913_3_bme_pgi/4233604‑4233889 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |