Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi526,8000AG30.3% 95.8 / 45.3 66E1180E (GAA→GAG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (14/32);  new base G (8/12);  total (22/44)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATAT  >  NC_000913_3_bme_pgi/526652‑526946
                                                                                                                                                     |                                                                                                                                                  
cgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGA‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGaa                                                                                                                                                    >  1:642772/1‑149 (MQ=255)
  cAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAata                                                                                                                                                  <  1:106093/149‑1 (MQ=255)
   aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAgtat                                                                                                                                                 <  1:109188/149‑1 (MQ=255)
      ggcggCTGACGGTGGAGCAACTGGCCAGACAGTTGGAGCCGGAATACACACCGGCGCGAAATGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                                                              <  2:85594/146‑1 (MQ=255)
            tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                        >  1:393030/1‑149 (MQ=255)
            tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACCGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                        <  2:293281/149‑1 (MQ=11)
             gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                       <  1:453957/149‑1 (MQ=255)
             gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                       >  2:470673/1‑149 (MQ=255)
             gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                                       <  2:174484/149‑1 (MQ=255)
             caCGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                       <  1:202039/148‑1 (MQ=255)
              ccGGGGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACCCACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgggg                                                                                                                                      <  2:27653/148‑1 (MQ=11)
              aCGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgggg                                                                                                                                      <  1:129547/149‑1 (MQ=255)
               cGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGc                                                                                                                                     >  1:676795/1‑149 (MQ=255)
                  tGGCGCAAGTGGCGAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                                  <  2:19287/149‑1 (MQ=11)
                  tGGAGCAACTGGCCAGACAGGTGGAGCTGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                                  <  1:632038/149‑1 (MQ=11)
                  tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACGGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                                                                  <  1:487332/149‑1 (MQ=255)
                     aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGGGGAAAGCTCATCTTTATCACTGCGACCGCCGG‑GGACTGCCGCGGGCGCTTATCAGCGAAGACGGCAGTGCGGCGTGGGGCGGGGAGTTGGATGGGTGGGGcggcccg                                                                                                                               >  2:286324/1‑143 (MQ=11)
                     aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAg                                                                                                                               >  1:562898/1‑149 (MQ=255)
                     aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAg                                                                                                                               <  1:314117/149‑1 (MQ=255)
                     aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATACGCGCGGAGGGGAATCTGGCGTGGCGCGGGGGGTGTGCTAGATGGGGCAGCCAg                                                                                                                               >  2:286313/1‑149 (MQ=11)
                      gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                              <  1:3407/149‑1 (MQ=255)
                         aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGTCAACCAGCTTa                                                                                                                           <  1:470673/149‑1 (MQ=255)
                          cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTaa                                                                                                                          >  2:220685/1‑149 (MQ=255)
                          cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTaa                                                                                                                          <  1:68987/149‑1 (MQ=255)
                           tGGCCGGACAGGTTGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAAt                                                                                                                         <  2:5689/149‑1 (MQ=255)
                           tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                         <  2:300337/149‑1 (MQ=255)
                             gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATTTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                       <  2:204484/149‑1 (MQ=255)
                              ccAGACAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                      <  2:210242/149‑1 (MQ=255)
                               cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                     >  1:447876/1‑149 (MQ=255)
                                 gACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgaggcg                                                                                                                   <  1:224028/149‑3 (MQ=255)
                                         gAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGca                                                                                                           <  2:160852/149‑1 (MQ=255)
                                          aGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAAt                                                                                                                                          <  1:238070/117‑1 (MQ=255)
                                          aGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAAt                                                                                                                                          >  2:238070/1‑117 (MQ=255)
                                          aGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCCGGCGCTTATCAGCGAAGACGGCAATACGGCGGGCGGCCCGGCATATAATGAATGGGGCAACCCGCTTAATGAGGCGAACCCGcat                                                                                                          >  2:321624/1‑149 (MQ=14)
                                               gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                     >  1:544192/1‑149 (MQ=255)
                                                       acCGGCGCGAAAAGGTCACCTTTATCACTGCGACCACCGGAAAAAAACAACAGGAGAGT‑GGAGGGGTGGCGGGAATACGGCGTGGAGAGCATAAGATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGc                                                                                             <  2:348028/149‑1 (MQ=9)
                                                               gAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                              >  1:205872/1‑92 (MQ=14)
                                                               gAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                              <  2:205872/92‑1 (MQ=14)
                                                               gAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGt                                                                                     >  1:653644/1‑149 (MQ=255)
                                                                      tCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                              >  1:589355/1‑149 (MQ=255)
                                                                      tCATCTTTATCACCGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAACAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                              <  1:26190/149‑1 (MQ=255)
                                                                        aTCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                                            <  1:371342/149‑1 (MQ=255)
                                                                          cTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGAGGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTTTCAGCGGTACCGGCTGCCAGGgca                                                                          >  2:425378/1‑149 (MQ=255)
                                                                               tCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcagc                                                                     >  1:288289/1‑149 (MQ=255)
                                                                                      gACCACCGG‑GGATTGCCGCGGCGGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGGGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                              <  2:118073/149‑1 (MQ=14)
                                                                                      gACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                              <  2:697405/149‑1 (MQ=255)
                                                                                      gACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                              <  2:494627/149‑1 (MQ=255)
                                                                                      gACCACCGG‑GGACTGCCGCTGGCGCCTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                              <  1:705123/149‑1 (MQ=255)
                                                                                        ccaccGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAgg                                                            <  2:646987/149‑1 (MQ=255)
                                                                                         caccGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                         >  1:690012/1‑103 (MQ=255)
                                                                                         caccGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                         <  2:690012/103‑1 (MQ=255)
                                                                                            cGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATTAATGGGGGAACCAGCGTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATc                                                        <  1:374160/149‑1 (MQ=255)
                                                                                            cGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATc                                                        <  2:562738/149‑1 (MQ=255)
                                                                                                      ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                               <  2:653644/149‑1 (MQ=255)
                                                                                                       cGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTAc                                              >  2:618736/1‑149 (MQ=255)
                                                                                                             ggcTGATCAGCGAAGACGCCAATACGGCGTGGAGCGGGGAATATGAGGAATGGGGCAACCACGTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAc                                        <  2:442588/148‑1 (MQ=17)
                                                                                                                 tATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTc                                    <  2:544192/149‑1 (MQ=255)
                                                                                                                   tCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCAc                                  <  1:202415/149‑1 (MQ=255)
                                                                                                                     aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                                >  1:94013/1‑149 (MQ=255)
                                                                                                                     aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                                <  1:425378/149‑1 (MQ=255)
                                                                                                                     aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                                >  1:397483/1‑149 (MQ=255)
                                                                                                                                   tACGGCGGGGAGCGGGGAATATGATGAATGGGGCAACCAGCGTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGGCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGt                  <  2:20750/149‑1 (MQ=255)
                                                                                                                                    gcGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGtt                 <  1:342125/148‑1 (MQ=255)
                                                                                                                                    actgCGTGGAACGCGTAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCCGCCGTACCGTCTTCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGtt                 <  1:184311/146‑1 (MQ=17)
                                                                                                                                    aCGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGtt                 <  1:220685/149‑1 (MQ=255)
                                                                                                                                      ggCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAAGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGc               >  1:453817/1‑149 (MQ=255)
                                                                                                                                        cGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGAATGTTTAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAg             <  1:335594/149‑1 (MQ=255)
                                                                                                                                                 cgGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCGAGGGCGGtat    >  1:110860/1‑149 (MQ=255)
                                                                                                                                                   gAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTatat  <  2:366676/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGG‑GGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATAT  >  NC_000913_3_bme_pgi/526652‑526946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑