Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,233,793 C→A intergenic (‑560/‑2114) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,233,7930CA97.6% 127.2 / NA 42intergenic (‑560/‑2114)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (20/21);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAACGACGA  >  NC_000913_3_bme_pgi/4233652‑4233936
                                                                                                                                             |                                                                                                                                               
tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGc                                                                                                                                          >  1:216687/1‑149 (MQ=255)
tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGc                                                                                                                                          <  1:249009/149‑1 (MQ=255)
 ttACACTCAACATTACGCTAACGGCACTAAAACCATCTCATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGGTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCt                                                                                                                                         <  2:278878/149‑1 (MQ=255)
         aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGc                                                                                                                                 >  1:601943/1‑149 (MQ=255)
          aCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGTAAGAGCCGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCa                                                                                                                                >  1:78485/1‑149 (MQ=255)
            aTTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCAtt                                                                                                                              <  1:451314/149‑1 (MQ=255)
             ttACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTg                                                                                                                             <  2:533286/149‑1 (MQ=255)
                 gCTAACGGCACTAAAACCATCACCTTTGTCTGTGACTGGCGCTACAATCGTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCg                                                                                                                         <  1:26749/149‑1 (MQ=255)
                 gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGCAGAGACTCGATATCTGCAAGAGACGCGCTTAAGCTTGAAGCAACGGTAGCAAGGCTTAAAAAGCATTGGGCg                                                                                                                         <  2:493753/149‑1 (MQ=255)
                  cTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCgg                                                                                                                        >  1:349913/1‑149 (MQ=255)
                     aCGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAAt                                                                                                                     >  2:376484/1‑149 (MQ=255)
                      cggctgtAATACCATCACATTTTGTTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAATATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGGTTGAAGCAATGGTAGCAAGGCTGAAAAAGCATTGGGGGGAATc                                                                                                                    <  2:506854/143‑1 (MQ=255)
                         cACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGc                                                                                                                                          >  2:580950/1‑124 (MQ=255)
                         cACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGc                                                                                                                                          <  1:580950/124‑1 (MQ=255)
                            tAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcccc                                                                                                              >  1:3977/1‑149 (MQ=255)
                                         tttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc                                                                                                 >  1:287238/1‑149 (MQ=255)
                                                  gACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGAAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCACGATAGGCGCACGGCATTc                                                                                        >  2:155949/1‑149 (MQ=255)
                                                          cTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGACACAACGGTAGCAAAGCTGAAAAAGCATTGGGCGAAATCGGCCCCCCGCGATATGACCGCGGCATTCAACGcctg                                                                                >  2:639151/1‑147 (MQ=255)
                                                          cTACAATCTTCCAAAGTCACAATTCTCAAAATAAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCCTTGGGCGGAATCGGCCCCGCGCGACATGCGCGCGGCGTGCAGCGCCGa                                                                                >  1:525935/1‑149 (MQ=255)
                                                              aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGAcccc                                                                            >  1:64734/1‑149 (MQ=255)
                                                                 cTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc                                                                         <  1:133769/149‑1 (MQ=255)
                                                                      aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt                                                                    >  2:394008/1‑149 (MQ=255)
                                                                          tCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg                                                                <  1:356239/149‑1 (MQ=255)
                                                                            acaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCg                                                              >  2:258130/1‑149 (MQ=255)
                                                                            acaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCGGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGCCCCCGCCCCATTCGGCCg                                                              >  2:326495/1‑149 (MQ=255)
                                                                             caATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGa                                                             <  2:349913/149‑1 (MQ=255)
                                                                                   tcAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCg                                                       >  1:643893/1‑149 (MQ=255)
                                                                                     aaaaTCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGct                                                     <  2:287238/149‑1 (MQ=255)
                                                                                       aaTCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctct                                                   <  2:189564/149‑1 (MQ=255)
                                                                                         tCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc                                                 <  2:430521/149‑1 (MQ=255)
                                                                                            gaagaGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTg                                              >  1:209376/1‑149 (MQ=255)
                                                                                                       cTAAGGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtcttct                                   <  1:40467/149‑1 (MQ=255)
                                                                                                           tGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTc                               <  1:376484/149‑1 (MQ=255)
                                                                                                               aaGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACt                           >  2:297442/1‑149 (MQ=255)
                                                                                                                 gagaCGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATATTCTGTTCGACTgg                         <  2:64734/149‑1 (MQ=255)
                                                                                                                  agaCGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGt                        <  2:3977/149‑1 (MQ=255)
                                                                                                                  agaCGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGt                        <  1:394008/149‑1 (MQ=255)
                                                                                                                      gcgAAGAAGCTTGAAGCAACGGAACCAAAGCTAAAAAAGCAGTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGaa                    <  2:589808/149‑1 (MQ=255)
                                                                                                                      gcaacgaACGTTGAAGCCTCGGTCCCAAAGCTGCAAAAGCATTGGGCGTAATCTGCCCCGCGCGATATGCGCGCGGCTGTCAGCGCCGACCCTGGTCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGaa                    <  2:482268/146‑1 (MQ=255)
                                                                                                                              gCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGGAGAGCgggg            >  2:657091/1‑149 (MQ=255)
                                                                                                                               cTTGAAGCAACGGTAGCAAAACTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCAATCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGGTCAACTGGTCGAAGTGCCGGGt           >  2:566218/1‑149 (MQ=255)
                                                                                                                                        aCGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAAcgacga  <  2:643236/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                               
TTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAACGACGA  >  NC_000913_3_bme_pgi/4233652‑4233936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: