Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	4,235,408	Δ1 bp	intergenic (‑2175/‑499)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	4,235,407	0	A	.	100.0%	210.1 / NA	47	intergenic (‑2174/‑500)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (17/30);  total (17/30)

TGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATT  >  NC_000913_3_bme_pgi/4235263‑4235550                                                                                                                                                 |                                                                                                                                                tGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCTGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGatcat                                                                                                                                             >  1:76401/1‑145 (MQ=255)  gCCTTCGATCCATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑atcatc                                                                                                                                           <  1:259063/149‑1 (MQ=255)     ttCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCAtcgtc                                                                                                                                        <  1:344337/149‑1 (MQ=255)     ttCGATCAATGGGGCGTGGAACTGGGTAAGGAACGGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCAtcgtc                                                                                                                                        <  2:271573/149‑1 (MQ=255)        gATCAATGGGGGGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGat                                                                                                                                     <  2:357235/149‑1 (MQ=255)        aaTCAGTGGGGCGGGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGGGTGGTCGCGACGCTGCCACGCAAGTGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGat                                                                                                                                     <  2:644544/148‑1 (MQ=255)         aTCAATGGGGCGTGGAACCGGGTAAAGAACAGGCGACGGAACTTTTGCCGGAAGTATCGGGCAAGGGAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGCAAGCTTG‑ATCATCGTCGata                                                                                                                                    >  1:344172/1‑149 (MQ=255)           cAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACCTTTGCCTGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATg                                                                                                                                  <  1:645683/149‑1 (MQ=255)               ggggCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAGGCTTG‑CTCATCGTCGATATGTAgg                                                                                                                              >  1:673115/1‑149 (MQ=255)                 ggCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAACGATACCGCTTGTTTTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGcc                                                                                                                            <  1:677771/149‑1 (MQ=255)                      ggAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATa                                                                                                                       >  2:199039/1‑149 (MQ=255)                        aaCTGGGTAAGGAACTGGCGACGTAACTTGTGCCGGGTGTATGGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                     <  2:71510/149‑1 (MQ=255)                        aaCTGGGTAAGGAACGGGCGACGGAACTTTGGCCGGTTGTAGCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGTGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                     <  2:236005/149‑1 (MQ=255)                        aaCTGGGGAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                     <  1:250314/149‑1 (MQ=255)                             ggTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGACATGTAGGCCGGATAAGGCGtt                                                                                                                >  1:382457/1‑149 (MQ=255)                                  ggAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                           >  1:62289/1‑149 (MQ=255)                                   gAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGCCCGGATCAGGCGTTCAcgcc                                                                                                          >  2:126717/1‑149 (MQ=255)                                                  cTTTTGCAGGTTGTAGCGGGTAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGa                                                                                           <  1:408166/149‑1 (MQ=39)                                                   ttttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGATTGTCGCGCATCTGCATGAGAGAAAGAAAGCTTG‑ATCATCATAGATATGTATACCGGATAAGTCTTTCACGCCGCATCCGGCAACCGAt                                                                                          >  2:408166/1‑149 (MQ=255)                                                   gtttGCCGGTTGGATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                          <  2:510344/148‑1 (MQ=255)                                                          ggTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCCACCGATGCCTGAt                                                                                   >  1:107942/1‑149 (MQ=255)                                                           gTTGTAGCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCATCCACGCAAGGGCGTGTCGCGCATCTTCATTCGCGAAGGAACGCTTG‑ATCATCGTCGCTATGATGGCCGCAGAAGCCGTTCACGCCCCAACCGCAAAACGATGcagaacg                                                                                  >  2:333820/1‑143 (MQ=255)                                                                   gggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCgg                                                                          >  1:170498/1‑149 (MQ=255)                                                                    ggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt                                                                         >  2:40737/1‑149 (MQ=255)                                                                       aaggaaggGGCGAGTGGTTGCGAAGATTCCACGCAAGGGCTTGTCGCGCATCTGAATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc                                                                      <  2:218265/149‑1 (MQ=38)                                                                         ggaaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                    >  2:145575/1‑149 (MQ=255)                                                                          gaaggGGCGAGTGGCCGCGACGCTCCCACGCAAGTGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCTTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                                                                   <  1:293481/149‑1 (MQ=38)                                                                            tggggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCtt                                                                 <  1:390045/148‑1 (MQ=255)                                                                              gggCGAGGGGTCGCGACGCTTCCACGCAAAGGACTGTCCCGGATCTTCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt                                                               <  1:190945/149‑1 (MQ=37)                                                                                gCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAgg                                                                                                                    <  2:574052/94‑1 (MQ=255)                                                                                gCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAgg                                                                                                                    >  1:574052/1‑94 (MQ=255)                                                                                gCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                             <  1:283496/149‑1 (MQ=255)                                                                                gCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                             <  2:344419/149‑1 (MQ=255)                                                                                   aGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                          <  1:322859/149‑1 (MQ=255)                                                                                          gcgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAgg                                                   >  2:227325/1‑149 (MQ=255)                                                                                           cgACGCTTCCACGCAAGGGCTTGTCGCGCACCTGCATGCGCGAAGGAAAGCTTG‑ATCAGCGTCGATATGTAGGCCGGAGAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                  <  2:313239/149‑1 (MQ=255)                                                                                           cgACGCTTCCACCCAAGGGCGTGTCGCGCATCTGCATTCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGCTAAGGCGTTCTCGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                  <  1:96682/149‑1 (MQ=25)                                                                                            gACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCCGGCCTACAgtca                                                 >  2:160715/1‑146 (MQ=255)                                                                                                 ttCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                            <  2:76401/149‑1 (MQ=255)                                                                                                    cACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                                         <  1:541642/149‑1 (MQ=255)                                                                                                            ggtgtGTCGCGCATCTGCATGCGCGAAGGAAAGCTTG‑AGCATCATCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTATAGGTCGATGCCGATATGTACa                                 <  2:609798/145‑1 (MQ=25)                                                                                                                tGTCGCGCATCTACATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCAGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATCCCCATATGTACATCGt                             >  2:226623/1‑149 (MQ=25)                                                                                                                   cgcgCATCTGCATGCGCGAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                          <  2:62289/149‑1 (MQ=255)                                                                                                                                 cgggAAGGAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACa            <  2:344172/146‑1 (MQ=255)                                                                                                                                   cgAAGGAAACCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATa          <  1:588508/149‑1 (MQ=255)                                                                                                                                       ggAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                          <  2:523015/97‑1 (MQ=255)                                                                                                                                       ggAAAGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                          >  1:523015/1‑97 (MQ=255)                                                                                                                                           aGCTTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt  <  1:145575/149‑1 (MQ=255)                                                                                                                                           aGCTTG‑ATCATCGTCGATATGCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTAATCAGGCCTACAGGACGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt  <  1:608117/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                TGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATT  >  NC_000913_3_bme_pgi/4235263‑4235550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑