Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	2,286,313	0	T	A	31.1%	92.3 / 70.4	74	intergenic (+26/+77)	proL/yejO	tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base T (22/29);  new base A (13/10);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCA  >  NC_000913_3_bme_pgi/2286165‑2286453                                                                                                                                                     |                                                                                                                                             ttACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGGCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCCCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTa                                                                                                                                             >  3:301043/1‑150 (MQ=255)         aaTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACGACACCTTACggttggt                                                                                                                                     >  2:721421/1‑150 (MQ=255)         aaTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttggt                                                                                                                                     >  3:134447/1‑150 (MQ=255)         aaTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttggt                                                                                                                                     <  4:134447/150‑1 (MQ=255)             ggTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGtttt                                                                                                                                  <  1:15356/149‑1 (MQ=255)             ggTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGtttt                                                                                                                                  >  2:15356/1‑149 (MQ=255)              gtgtGGAAAACGGTAGTACTAGCAGCCACGACTCGGCACGTAGCGCAGTCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAACTCCCAAGAAAAAACCAACCCTTCCGGTTGGtttttt                                                                                                                                <  4:252145/150‑1 (MQ=255)               tgtgGAAATCGGTAGTATTAGCATGCACGATTTGGCACGTATCGTATCGGTTTATCGCACCGTGATTGTGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                                                               <  1:721421/150‑1 (MQ=255)                   gacaaCGGTAGTATTAGCAGCCACGAGGCGGCACGTAACGCAGCCTGGTAGCGCACCGTCGTGGGGGGTCGGGGGGCGGAGGTTCAGATGCGCTCGTGCCGACCAGAAATCCCAAGGAAAAACAAACCCTTAAGGTTGGTTGTTTTatat                                                                                                                           >  4:384568/4‑150 (MQ=255)                      aaCGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTg                                                                                                                        <  3:172645/150‑1 (MQ=255)                               ttcggcagcCACGAGTCGGCACGTAGCGCACCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCTTGATAATACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaatt                                                                                                               <  3:197849/146‑1 (MQ=255)                                   gcagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGAt                                                                                                           >  2:711989/1‑150 (MQ=255)                                    cagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGGTTTTTTATATCTGCAATTAATTcagat                                                                                                          >  4:197849/1‑146 (MQ=255)                                     agcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttctt                                                                                                                             <  4:386439/130‑4 (MQ=39)                                     agcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttctt                                                                                                                             >  3:386439/1‑127 (MQ=39)                                         aCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                                                               <  1:122747/124‑1 (MQ=255)                                         aCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                                                               >  2:122747/1‑124 (MQ=255)                                         aCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAg                                                                                                     >  2:855449/1‑150 (MQ=255)                                           gAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCGAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGAc                                                                                                   <  1:825885/150‑1 (MQ=255)                                            aGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGatc                                                                                                  >  3:113781/1‑148 (MQ=255)                                              tCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGt                                                                                                <  3:494278/150‑1 (MQ=255)                                                ggCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGa                                                                                              <  3:454075/150‑1 (MQ=255)                                                ggCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGa                                                                                              >  2:279284/1‑150 (MQ=255)                                                                tGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGtt                                                                              <  1:162500/150‑1 (MQ=255)                                                                 ggTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAATCTCCCAAGACAAAACCAACCCTTACGGTTGGTTTTTTTATATCTCCCATTCATTCTATGTTCAGACCGTGACACATCACAGCCTGttt                                                                             <  3:384568/150‑1 (MQ=255)                                                                  gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTa                                                                            >  1:541898/1‑150 (MQ=255)                                                                       gcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTc                                                                       <  1:279284/150‑1 (MQ=255)                                                                         aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTtat                                                                                                                             <  1:824003/94‑1 (MQ=255)                                                                         aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTtat                                                                                                                             >  2:824003/1‑94 (MQ=255)                                                                         aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGGCACATCACAGCCTGTTTATTTTCTg                                                                     >  3:344140/1‑150 (MQ=255)                                                                               aTGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaatt                                                                                                               <  3:300932/102‑1 (MQ=255)                                                                               aTGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaatt                                                                                                               >  4:300932/1‑102 (MQ=255)                                                                                tGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGGCGACCAAAAATCCCAAGAAAACACCAACCCATACGGTTGGTTTTTTTATATCTGCAATTACTTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAg                                                              <  1:215094/150‑1 (MQ=255)                                                                                       tCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTcc                                                       <  1:280040/150‑1 (MQ=255)                                                                                         gggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGGCTGTTTAttt                                                                         <  1:210668/130‑1 (MQ=255)                                                                                         gggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGGCTGTTTAttt                                                                         >  2:210668/1‑130 (MQ=255)                                                                                          ggggTCGGAGGTTCAAATCCTCTCGTGCCGACAAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGa                                                    >  4:299426/1‑150 (MQ=255)                                                                                             gTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttggtt                                                                                                                                    <  4:102434/67‑1 (MQ=255)                                                                                             gTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCATTACggttggtt                                                                                                                                    >  3:102434/1‑67 (MQ=255)                                                                                                         aaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAg                                     <  1:855449/150‑1 (MQ=255)                                                                                                           tCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTAt                                                                 <  1:486937/120‑1 (MQ=255)                                                                                                           tCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTAt                                                                 >  2:486937/1‑120 (MQ=255)                                                                                                              tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTc                                <  1:711989/150‑1 (MQ=255)                                                                                                                tcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCa                              >  1:267406/1‑150 (MQ=255)                                                                                                                  gTGCCGACCAAAAAGCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCAATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACg                            <  3:90910/150‑1 (MQ=255)                                                                                                                            aaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGGCACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTAc                  <  4:344140/150‑1 (MQ=255)                                                                                                                             aaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTAcc                 >  1:559015/1‑150 (MQ=255)                                                                                                                              aaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCCCACCCGCCTGAGTATTCCACGGCGATTCTAccc                <  2:541898/150‑1 (MQ=255)                                                                                                                                    aaGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgcgc          >  1:507313/1‑150 (MQ=255)                                                                                                                                    aaGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGACGATTCTACCCCTgcgc          <  4:301043/150‑1 (MQ=255)                                                                                                                                      aaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCa        >  2:474830/2‑150 (MQ=255)                                                                                                                                       aaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCTATAAACAGACCGTGACACATCACAGCCTGttt                                                                             >  3:262787/1‑80 (MQ=37)                                                                                                                                       aaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCTATAAACAGACCGTGACACATCACAGCCTGttt                                                                             <  4:262787/80‑1 (MQ=37)                                                                                                                                       aaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAg                                                              >  1:553970/1‑95 (MQ=38)                                                                                                                                       aaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAg                                                              <  2:553970/95‑1 (MQ=38)                                                                                                                                       aaaaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGcc         <  1:296240/148‑1 (MQ=255)                                                                                                                                       aaaaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGGTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGcc         >  2:296240/1‑148 (MQ=255)                                                                                                                                        aaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACAt                                                                                         <  3:349551/67‑1 (MQ=25)                                                                                                                                        aaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACAt                                                                                         >  4:349551/1‑67 (MQ=25)                                                                                                                                        aaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCa                                                               <  1:830824/93‑1 (MQ=38)                                                                                                                                        aaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCa                                                               >  2:830824/1‑93 (MQ=38)                                                                                                                                        aaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCacac                                              <  1:94625/110‑1 (MQ=255)                                                                                                                                        aaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCacac                                              >  2:94625/1‑110 (MQ=255)                                                                                                                                        aaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCAtt      >  2:612182/1‑150 (MQ=255)                                                                                                                                        aaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCAt       <  1:390735/149‑1 (MQ=255)                                                                                                                                        aaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCAt       >  2:390735/1‑149 (MQ=255)                                                                                                                                        aaaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCAtt      <  2:74236/150‑1 (MQ=255)                                                                                                                                         aaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                                                                <  3:504543/91‑1 (MQ=38)                                                                                                                                         aaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                                                                >  4:504543/1‑91 (MQ=255)                                                                                                                                         aaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTg     >  3:60302/1‑150 (MQ=255)                                                                                                                                         aaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTg     <  2:99301/150‑1 (MQ=255)                                                                                                                                          aaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCt                                        <  2:170756/114‑1 (MQ=255)                                                                                                                                          aaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTAGCAGAACGTCCAGACCACACCCGCCt                                        >  1:170756/1‑114 (MQ=255)                                                                                                                                            accaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCa  <  3:299426/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                             TTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCA  >  NC_000913_3_bme_pgi/2286165‑2286453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑