Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_bme_pgi 1,715,794 G→A 9.5% N32N (AAC→AAT)  pdxY ← pyridoxamine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi1,715,7940GA 9.5% 127.5 / 4.0 51N32N (AAC→AAT) pdxYpyridoxamine kinase
Reads supporting (aligned to +/- strand):  ref base G (16/30);  new base A (2/3);  total (18/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-01

ACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCAC  >  NC_000913_3_bme_pgi/1715647‑1715939
                                                                                                                                                    |                                                                                                                                                 
aCTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTAAGCGGCCAGACGt                                                                                                                                                  >  1:100048/1‑149 (MQ=255)
 cTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAACTGACTGGGCGTCATCACGCAGCCAGTCCATTTGACATTCTGGGTGTGCTTACAAAATTTAA‑CGCTGTTCAGCGGCCAGGCGtt                                                                                                                                                 >  2:411092/1‑149 (MQ=255)
  ttAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTc                                                                                                                                                <  1:368971/149‑1 (MQ=255)
  ttAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTc                                                                                                                                                >  2:102874/1‑149 (MQ=255)
      tACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCgcgc                                                                                                                                            <  1:510485/149‑1 (MQ=255)
             tCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCAAAATTTCGGTTAAAGGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCCGACGTTCGCGCCCAggcg                                                                                                                                     >  1:629914/1‑149 (MQ=255)
                   gtgtgtAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCAt                                                                                                                               <  1:346068/149‑1 (MQ=255)
                   gtgggcaaTTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGTTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCAt                                                                                                                               <  2:236856/143‑1 (MQ=255)
                    tgtgtAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATc                                                                                                                              >  2:785589/1‑149 (MQ=255)
                    tgtgtAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATc                                                                                                                              <  1:628760/149‑1 (MQ=255)
                    tgtgtAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATc                                                                                                                              <  1:505559/149‑1 (MQ=255)
                    tgtggAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGTCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATc                                                                                                                              <  1:31737/149‑1 (MQ=255)
                         aaTTTATTAATGGCTGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGaaa                                                                                                                         <  2:823467/149‑1 (MQ=255)
                         aaTTTATCCATGGCTGGAATGCCTTGAACACGTGCGGTTAACTGGCTGGGCGGCCTCACGCAGCCAGTCCATTTGCTGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGaaa                                                                                                                         <  2:246044/149‑1 (MQ=255)
                         aaTTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTTGGTGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGaaa                                                                                                                         <  1:267148/149‑1 (MQ=255)
                          aTTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAAc                                                                                                                        >  1:586461/1‑149 (MQ=255)
                               tCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATAGGAAACTCTGc                                                                                                                   <  1:551362/149‑1 (MQ=255)
                                cgaTGGCGGCAATGCCTTGCACAATTTCGGGTCAATGGCTGGGCGGCATGAGGCAGAGAGGCCATTTGTCGCAGGGGGTGTGATTAGAAAATGGAA‑CGGTGTTCAGCGGGCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGcc                                                                                                                  <  2:209810/147‑1 (MQ=255)
                                   tgacggcAATGCCTTGCACAATTTCGGTTAAAGGGCTGGGCGACATCACGCAGCCAGTCCATTTGCCGTATTGGGAGTGATTAGAAAATTGAA‑CGGTCTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCa                                                                                                               >  2:853518/4‑149 (MQ=255)
                                         cAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTa                                                                                                         >  2:241575/1‑149 (MQ=255)
                                            tGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTAccc                                                                                                      >  1:219302/1‑149 (MQ=255)
                                            tGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACATTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTAccc                                                                                                      >  1:299829/1‑149 (MQ=255)
                                            tGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACATTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTAccc                                                                                                      >  1:298981/1‑149 (MQ=255)
                                             gCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGACGTATTGGGTGTGATTAGAAAATTGAA‑AGGTGTTCAGCGGCCAGACGTTGGCGCGCAGACGGCGCATCGGACACTCTGCCGCACTGTTAACCg                                                                                                     >  2:79959/1‑149 (MQ=255)
                                                ttGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACATTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCAt                                                                                                  <  2:299829/149‑1 (MQ=255)
                                                ttGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACATTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCAt                                                                                                  <  2:298981/149‑1 (MQ=255)
                                                ttGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAAGAAATTGAA‑CGGTGTACAGCGACCATACGTTCGCGCCAAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCAt                                                                                                  >  1:131761/1‑149 (MQ=255)
                                                ttGCACAATTTCGGTTAAATAGCTGGGACGCACCACGCACCCAGTCCAGTTGCCGTATTGGGTGTGATTACAAAATTGAA‑CGGTGTCCAGCGGCCAGACACTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTCTTACCCGCAt                                                                                                  <  2:494470/149‑1 (MQ=255)
                                                ttGCACAACTTCTGTTAAATGGCTGGGCGGCATAAGCCAGCCAGTTCATATGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCAt                                                                                                  <  1:796376/149‑1 (MQ=255)
                                                         ttCGTTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATaaa                                                                                         <  2:293871/149‑1 (MQ=255)
                                                                 aaTGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTga                                                                                 >  1:421245/1‑149 (MQ=255)
                                                                   tGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTgaga                                                                               >  2:687436/1‑149 (MQ=255)
                                                                        gggCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGTCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGa                                                                          <  1:900867/149‑1 (MQ=255)
                                                                               aTCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCgag                                                                   >  1:288678/1‑149 (MQ=255)
                                                                                     cAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGATAATTGAG‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATAtt                                                             <  1:517144/149‑1 (MQ=255)
                                                                                      aGCCAGTCCATTTGACGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATAttt                                                            <  1:501023/149‑1 (MQ=255)
                                                                                          aGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTtca                                                        <  1:241575/149‑1 (MQ=255)
                                                                                                 tttccGTATTGGGTGTGATTAGACAATTTAATCTGT‑ATAAGCGCCATGACGGTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGtt                                                 <  2:409958/146‑1 (MQ=255)
                                                                                                       tATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCCGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGAGAGAATATTTTTCATCATGTTCCCTGt                                           >  1:211803/1‑149 (MQ=255)
                                                                                                                 gATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAATCACCGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCa                                 <  1:623945/149‑1 (MQ=255)
                                                                                                                 gATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCa                                 <  1:687494/149‑1 (MQ=255)
                                                                                                                  aTTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGCCCACAAACAACGTGAGGCCGCAGAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAg                                <  1:663103/149‑1 (MQ=255)
                                                                                                                  aTTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAg                                <  2:202831/149‑1 (MQ=255)
                                                                                                                   agaggaaaGTGAG‑CGGTGGTCAGCGGCCAGACGTTCGCGCCTAGGGGGCGCAGCGGAAACTCTGCCGCACTGTTATCCGCATGACCATAGACAGCGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAgg                               <  2:4108/144‑1 (MQ=255)
                                                                                                                       aaaaTTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCTCCCAGGCGGCGCGTTGGAAGCTCTGCCGCACTGTTACCCGGATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGa                           <  1:119073/149‑1 (MQ=255)
                                                                                                                         aaTTGAT‑CGGTGTTCAGCGGCCAGTCGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGt                         <  1:102874/149‑1 (MQ=255)
                                                                                                                         aaTTGAA‑CGGTGTTAAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGAACATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGt                         <  1:208378/149‑1 (MQ=255)
                                                                                                                            ggAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCTGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAAAACGGGTCATCATATTCCCTGGATAAAAACCAGGGGGAGTGAt                      <  2:601600/148‑1 (MQ=255)
                                                                                                                              aa‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCTATGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGAttt                    <  1:853518/149‑1 (MQ=255)
                                                                                                                                                aGACGTTCGGGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCa   <  2:4092/149‑1 (MQ=255)
                                                                                                                                                 gACGTTCGCGCCCAGGCGGCGCATCGGAAGCTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCAc  >  1:761333/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAA‑CGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCAC  >  NC_000913_3_bme_pgi/1715647‑1715939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑