Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_bme_pgi 1,971,929 A→C 8.6% S255A (TCA→GCA)  tar ← methyl‑accepting chemotaxis protein II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi1,971,9290AC 8.6% 105.8 / 4.4 50S255A (TCA→GCA) tarmethyl‑accepting chemotaxis protein II
Reads supporting (aligned to +/- strand):  ref base A (22/22);  new base C (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-01

CAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAG  >  NC_000913_3_bme_pgi/1971782‑1972076
                                                                                                                                                   |                                                                                                                                                   
cAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGaa                                                                                                                                                    <  1:486805/149‑1 (MQ=255)
   tttCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGCACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACg                                                                                                                                                 >  1:399543/1‑149 (MQ=255)
   tttCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCTCGGACATGAGTGACGGTGTCAGCCAACGAGCGTTGCATATTTGGAACg                                                                                                                                                 >  2:465738/1‑149 (MQ=255)
      cttcCAGCGCGGATGCCTGATGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGctc                                                                                                                                              <  1:579037/149‑1 (MQ=255)
        tcCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATTGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTTCACATATGCAACGctctt                                                                                                                                            <  2:47611/149‑2 (MQ=255)
         ccAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCGTTCAAAGAGCGTTGCATATGTGAAACGCTCTgc                                                                                                                                           >  1:243742/1‑149 (MQ=255)
            gcgcGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGTTGTCAGTCAAAGAGAGTTGCATATGTGAAACGCTCTGCGcc                                                                                                                                        >  2:595359/1‑149 (MQ=255)
            gcgcGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTATCAGTCAAAGAGCGTTGCATATGTGACACGCTCTGCGcc                                                                                                                                        >  1:179892/1‑149 (MQ=255)
             cgcgTATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGTCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGATGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCa                                                                                                                                       <  1:628238/149‑1 (MQ=255)
                 gATGCCTGCTTGTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTc                                                                                                                                   <  2:308972/149‑1 (MQ=255)
                      ctgctgTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCa                                                                                                                              >  1:317067/1‑149 (MQ=255)
                      ctgctgTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAACGTTGCATATGTGAAACGATCTGCGCCAGGTCGCCCa                                                                                                                              >  1:117324/1‑149 (MQ=255)
                       tgctgTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCAt                                                                                                                             >  1:827460/1‑149 (MQ=255)
                         ctgTTCAGTACGGGAGGAAAGGTCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCAttt                                                                                                                           <  2:518796/149‑1 (MQ=255)
                          tgTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTAGCGGACTAGATGGCATCTAAACATTCGCGGACCTGAGTGACGGTATCAGTCAAAGAGCGTTCCATCACTGAAACGATCTGCGCCAGGTCGCCCATTTc                                                                                                                          >  2:323988/1‑149 (MQ=255)
                          tgTTCAGTACGGGACGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGCTGGCACCTGAACCTTCGCGGACCTGAGTGACGGTGCCAGTCAAACAGCGTTGCATATGAGAACCCCTCTCAGCCCGGTccccacattc                                                                                                                          >  2:714659/1‑143 (MQ=255)
                           gTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCa                                                                                                                         <  1:397037/149‑1 (MQ=255)
                                             gATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTc                                                                                                       <  1:239673/149‑1 (MQ=255)
                                              ctCGATGTCGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCAATTTCACTGCGCCCGTCAATGGTCa                                                                                                      >  2:685876/2‑149 (MQ=255)
                                              aTCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGAATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCa                                                                                                      >  1:701580/1‑149 (MQ=255)
                                                ccgtgtTGCCCGCCGTAATTTCACGGGTACCTGCATACATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAgg                                                                                                    <  1:108568/147‑1 (MQ=255)
                                                          cgccgcAATTTCCCGGGTACCTGCATAGATGTCACCTGAAGATTCGCTGACATGAGGGACGGGGTCAGTCAAAGAGCGTTGCATATGTGAAACGTTCTGCTCCAGGTTCCCCATCTCACGGCGCCCGTCAATGGTCAGGGTATTCGcga                                                                                          <  2:506609/149‑3 (MQ=255)
                                                          cgccgcAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCa                                                                                          <  1:750490/149‑1 (MQ=255)
                                                             cgcAAGTGCACGGGATCCGGCATTGATGGCATCTGAACCTGCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGt                                                                                       <  2:518948/149‑1 (MQ=255)
                                                                  tttCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTacca                                                                                  >  1:782809/1‑149 (MQ=255)
                                                                      aCGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCgg                                                                              >  1:828229/1‑149 (MQ=255)
                                                                           tACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGCAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGAtt                                                                         >  1:412698/1‑149 (MQ=255)
                                                                           tACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCACATTTCACTGAGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGAtt                                                                         >  1:36574/1‑149 (MQ=255)
                                                                                  aTAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGaa                                                                  <  2:599509/149‑1 (MQ=255)
                                                                                  aTAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGaa                                                                  <  1:858337/149‑1 (MQ=255)
                                                                                   tAGATGGCATCTGAACCTTCGCGGAGATGAGTGACGGCGTAAGTCAAAAAGAGATGCATATGTGAAGCGCTATGAGCCAGGTCGCCCATTTCAGTGCGCCCGTCAATGGTCAGGGGGATCGCCAGGTTACGACCAGCGAGTTAGCGagt                                                                 >  2:375358/1‑147 (MQ=255)
                                                                                         gCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCGGGTCGCCCATTTCACTTCGACCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGGGAGc                                                           >  1:50349/1‑149 (MQ=255)
                                                                                            tCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCaat                                                        >  2:10027/1‑149 (MQ=255)
                                                                                              tGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTTCACATATGCAACGctcttt                                                                                                                                           >  1:578830/1‑62 (MQ=18)
                                                                                              tGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTTCACATATGCAACGctcttt                                                                                                                                           <  2:578830/64‑3 (MQ=18)
                                                                                                   cTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAAttttt                                                 <  1:10027/149‑1 (MQ=255)
                                                                                                   cTTCGCGGACATGAGTGACGGTGGCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCGTTTCACTGCGCCGGTCAATGGTCGGGGTATTCGCGAGGTTACCACCGGCGATTTCGCGAGTGTGCGTAATAAttttt                                                 >  2:554370/1‑149 (MQ=255)
                                                                                                                agggACGGGGTCAGTCAAAAGGCGTTGCATATGTGAAACGCTGTGCGCAGGGTCGCGCATTTCACTGCGCCCGTCAATGGTCAGTGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTaa                                    <  2:317067/146‑1 (MQ=255)
                                                                                                                 gTGGCGGTGTCAGTCAAAGGGCGTGGGATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAg                                   <  1:395632/149‑1 (MQ=255)
                                                                                                                  tGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTACGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCATTAATTTTTGCCAGCGGAGTaatc                                  >  2:418748/1‑147 (MQ=255)
                                                                                                                    aCGGTTTCAGTCAAATAGCGTTGCATATGTGAAACGCTCTGCCCCAGGTCGCCCATTTCCCTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCaa                                <  2:384238/149‑1 (MQ=255)
                                                                                                                        tgtCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATa                            <  1:749599/149‑1 (MQ=255)
                                                                                                                        tgtCAGGCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATGCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATa                            <  2:729500/149‑1 (MQ=255)
                                                                                                                         gtcagtcaAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTTCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGAAATAATTTTTGCCAGCGGAGTAAGCAACATAc                           >  1:363377/1‑149 (MQ=255)
                                                                                                                          tcagtcaAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTa                                     >  2:463461/1‑138 (MQ=255)
                                                                                                                          tcagtcaAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTa                                     <  1:463461/138‑1 (MQ=255)
                                                                                                                                aaaGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTAGCACATTTCACTGCGCCCGTCAAAGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGACTGTGGGCAATAATTATTGCCAGCGGAAGAGGCAACATAAGCCGAAt                    >  2:31225/1‑149 (MQ=255)
                                                                                                                                    tgCGTTGCATATTTGAAACGCTCGGCGCCAGGTCGCCCATTTCACTGAGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCg                <  2:856318/148‑1 (MQ=255)
                                                                                                                                          gcttatGTGTTACGTTTTGCGCTAGGTCGTTCATTTCTCTGCGGCCGTCAATGGTCGGGGTATTCGCCAGGTTACGAGCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCAc          <  1:169955/146‑1 (MQ=255)
                                                                                                                                                 tgAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGTGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGccacca   <  1:22736/149‑1 (MQ=255)
                                                                                                                                                  gAAACGCTCTGCGCCAGGTCGCCCAGTTCACTGCGGCCGTGAATGGTCAGTGTATTCGCGAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGAAAATGAAGCAACATATGGCGAATGCCGTACCACGCCACcag  <  2:828896/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAG  >  NC_000913_3_bme_pgi/1971782‑1972076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑