Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	1,927,949	A→C	R297R (CGT→CGG)	ptrB ←	protease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	1,927,949	0	A	C	96.1%	162.2 / ‑1.3	51	R297R (CGT→CGG)	ptrB	protease II
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (27/22);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

CTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCCGCGTCCAGCAGGCG  >  NC_000913_3_bme_pgi/1927802‑1928092                                                                                                                                                    |                                                                                                                                                cTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCg                                                                                                                                               >  2:112376/1‑150 (MQ=255)   tGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGt                                                                                                                                             >  1:258794/1‑150 (MQ=255)             ttGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCaaa                                                                                                                                   >  4:190552/1‑150 (MQ=255)                  gcAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGtttt                                                                                                                              >  2:17222/1‑150 (MQ=255)                        tGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGt                                                                                                                        >  2:539130/1‑150 (MQ=255)                          gTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTgg                                                                                                                      <  1:539130/150‑1 (MQ=255)                          gTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTgg                                                                                                                      >  2:322408/1‑150 (MQ=255)                           ttAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGc                                                                                                                     <  3:324948/150‑1 (MQ=255)                                ccGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTg                                                                                                                >  4:387950/1‑150 (MQ=255)                                ccGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTg                                                                                                                >  4:34895/1‑150 (MQ=255)                                ccGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTg                                                                                                                <  2:258794/150‑1 (MQ=255)                                ccGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTGTTTGCCGTGGCGGTTg                                                                                                                <  3:387950/150‑1 (MQ=255)                                       gACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCa                                                                                                         >  4:259029/1‑150 (MQ=255)                                       gACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCa                                                                                                         <  1:4707/150‑1 (MQ=255)                                         cGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGa                                                                                                       <  1:322408/150‑1 (MQ=255)                                          gCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGAt                                                                                                      >  1:107779/1‑150 (MQ=255)                                          gCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGAt                                                                                                      >  2:619414/1‑150 (MQ=255)                                            tctTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATaa                                                                                                    <  3:34895/150‑1 (MQ=255)                                                     accaGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTTGAACGCAGATAAAAACGATGc                                                                                           >  4:279176/1‑150 (MQ=255)                                                      ccagccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCa                                                                                                                                     <  4:360569/107‑1 (MQ=255)                                                      ccagccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCa                                                                                                                                     >  3:360569/1‑107 (MQ=255)                                                       cagccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTg                                                                                         >  1:340616/1‑150 (MQ=255)                                                         gccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGt                                                                                       >  1:21251/1‑150 (MQ=255)                                                                    aaCAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAgg                                                                            >  1:105226/1‑150 (MQ=255)                                                                    aaCAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAgg                                                                            <  3:394803/150‑1 (MQ=255)                                                                      cAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCt                                                                          >  1:465612/1‑150 (MQ=255)                                                                           tAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTAtt                                                                     >  2:288512/1‑150 (MQ=255)                                                                              aCCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGt                                                                  <  2:492271/150‑1 (MQ=255)                                                                              aCCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGt                                                                  <  4:356468/150‑1 (MQ=255)                                                                                  ttCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATc                                                              >  4:272756/1‑150 (MQ=255)                                                                                  ttCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATc                                                              <  3:266434/150‑1 (MQ=255)                                                                                      aGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACg                                                                                                           >  1:190958/1‑101 (MQ=255)                                                                                      aGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACg                                                                                                           <  2:190958/101‑1 (MQ=255)                                                                                              gTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcggcgcgg                                                  <  2:107779/150‑1 (MQ=255)                                                                                              gTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcggcgcgg                                                  <  1:619414/150‑1 (MQ=255)                                                                                                tttCGCGTGGCGGTCTTCATTCTTCGCACTGTTCCTCATCACGCAGACGGGTCCGGTATAAGCCAAAGTTTTTGCAGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcggcgcggcc                                                >  2:1327/1‑149 (MQ=255)                                                                                                    gcgTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGaaa                                            <  3:299511/150‑1 (MQ=255)                                                                                                    gcgTGGCGGAATTAACTCTGCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTCGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGaaa                                            >  2:510301/1‑150 (MQ=255)                                                                                                           ggAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAaaacaaac                                     >  2:430335/1‑150 (MQ=255)                                                                                                                      ttCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATaaa                                                                                                   <  4:207935/77‑1 (MQ=255)                                                                                                                      ttCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATaaa                                                                                                   >  3:207935/1‑77 (MQ=255)                                                                                                                       tCCCACTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCg                         <  2:475660/150‑1 (MQ=255)                                                                                                                         ccACTGTGGCTCATCACGCGTACGGGTCCGGTATGAGCCAAAGGTTTTGCCGTGGCGGTTGGAACGCAGATAAAGACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTGGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCggt                       <  2:398008/150‑2 (MQ=255)                                                                                                                           aCTGTTGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGATTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCa                     >  3:358976/1‑150 (MQ=255)                                                                                                                              gTTGCTCATCACGCATACGGGTCCGGTATAAGCCTAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCAttt                  <  2:463531/150‑1 (MQ=255)                                                                                                                               ttGCTCATCACGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTc                 <  1:17222/150‑1 (MQ=255)                                                                                                                                   tcatcaCGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCcgcg             >  2:694027/1‑150 (MQ=255)                                                                                                                                       caCGCATACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGCAGTGACCAAGGCTGTATTCGTGCTCTTTGCGGCGCGGCAGAAACACAACCGGCTCGGCATCGGCCATTTCCCCGcccc         >  2:659505/1‑149 (MQ=255)                                                                                                                                           cATGCGGGGCCGGTATAAGCCAAAGTTTTGGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCCGCGTCcagcag     <  1:659505/150‑1 (MQ=255)                                                                                                                                             tACGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCCGCGTCCAGCAGGc   <  1:215000/150‑1 (MQ=255)                                                                                                                                              aCGGGTCCGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCCGCGTCCAGCAGGCg  >  2:349021/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                CTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAACAAACGGCTCGGCATCGGCCATTTCCGCGTCCAGCAGGCG  >  NC_000913_3_bme_pgi/1927802‑1928092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑