Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,159,046 T→G L186R (CTG→CGG)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,159,0460TG98.0% 177.2 / ‑5.8 52L186R (CTG→CGG) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base G (29/21);  minor base A (0/1);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCA  >  NC_000913_3_bme_pgi/4158904‑4159188
                                                                                                                                              |                                                                                                                                              
ggCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAggg                                                                                                                                         >  4:197699/1‑150 (MQ=255)
  cAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGa                                                                                                                                       >  4:312418/1‑150 (MQ=255)
          aTTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAActgc                                                                                                                               <  4:306942/150‑1 (MQ=255)
               gTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATg                                                                                                                          <  1:623819/150‑1 (MQ=255)
                       cgcgCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGa                                                                                                                  >  2:80298/1‑150 (MQ=255)
                         cgcTGGTAAAAGAGAGCGAAGCATTCATTGAAGGGCCGTTGTTGGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCAAACCGCGAATGCGTACCGATGGCCGAAAAAAAAAAAAAAAAACTGCTGATGCTGGAAGATg                                                                                                                <  4:96761/150‑1 (MQ=255)
                                gAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTg                                                                                                         >  1:550564/1‑150 (MQ=255)
                                   agagagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGttt                                                                                                      >  2:495973/1‑150 (MQ=255)
                                       agCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCGCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcgc                                                                                                  <  4:360461/150‑1 (MQ=255)
                                       agCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcgc                                                                                                  <  3:405372/150‑1 (MQ=255)
                                                   attGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATg                                                                                      >  1:652372/1‑150 (MQ=255)
                                                       aaGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGtt                                                                                  >  2:320810/1‑150 (MQ=255)
                                                        aGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt                                                                                 <  3:197699/150‑1 (MQ=255)
                                                        aGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt                                                                                 <  1:120846/150‑1 (MQ=255)
                                                             cGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtt                                                                            >  2:143626/1‑150 (MQ=255)
                                                                  tttGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGaa                                                                       <  1:708949/150‑1 (MQ=255)
                                                                   ttGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAg                                                                      >  2:296035/1‑150 (MQ=255)
                                                                        gaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCggg                                                                 >  3:225117/1‑150 (MQ=255)
                                                                                gTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGTCGGATGa                                                         <  2:704931/150‑1 (MQ=255)
                                                                                     tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATa                                                    <  4:118371/150‑1 (MQ=255)
                                                                                     tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATa                                                    <  1:264650/150‑1 (MQ=255)
                                                                                        ctatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacac                                                 >  2:158008/1‑150 (MQ=255)
                                                                                          atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacacac                                               <  3:312418/150‑1 (MQ=255)
                                                                                              atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTcc                                           >  2:618786/1‑150 (MQ=255)
                                                                                               tGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATc                                                                                              >  1:187588/1‑98 (MQ=255)
                                                                                               tGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATc                                                                                              <  2:187588/98‑1 (MQ=255)
                                                                                                 aaGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                               >  4:169479/1‑95 (MQ=255)
                                                                                                 aaGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                               <  3:169479/95‑1 (MQ=255)
                                                                                                  aGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcgcgc                                       <  2:15336/150‑1 (MQ=255)
                                                                                                    aTCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGa                                     <  2:550564/150‑1 (MQ=255)
                                                                                                       aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGa                                                            >  1:431697/1‑124 (MQ=255)
                                                                                                       aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGa                                                            <  2:431697/124‑1 (MQ=255)
                                                                                                       aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCa                                  >  1:513965/1‑150 (MQ=255)
                                                                                                         ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGc                                <  2:509130/150‑1 (MQ=255)
                                                                                                          cGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGcc                               >  3:184273/1‑150 (MQ=255)
                                                                                                          cGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGcc                               >  2:12993/1‑150 (MQ=255)
                                                                                                           gTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCt                              <  1:618786/150‑1 (MQ=255)
                                                                                                               gCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt                                                                                 <  2:610571/95‑1 (MQ=255)
                                                                                                               gCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt                                                                                 >  1:610571/1‑95 (MQ=255)
                                                                                                               gCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                <  2:99319/112‑1 (MQ=255)
                                                                                                               gCGAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                >  1:99319/1‑112 (MQ=255)
                                                                                                                 gAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                          >  3:433205/1‑132 (MQ=255)
                                                                                                                 gAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                          <  4:433205/132‑1 (MQ=255)
                                                                                                                 gAACCGCGAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAc                        >  3:34963/1‑150 (MQ=255)
                                                                                                                     cgcgAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTg                    >  4:4278/1‑150 (MQ=255)
                                                                                                                      gcgAATGCGTACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc                   >  4:383831/1‑150 (MQ=255)
                                                                                                                               tACCGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg          >  1:136272/1‑150 (MQ=255)
                                                                                                                                  cGATGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg       >  2:121654/1‑150 (MQ=255)
                                                                                                                                    aTGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCg                                                                   >  2:382502/1‑88 (MQ=255)
                                                                                                                                    aTGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCg                                                                   <  1:382502/88‑1 (MQ=255)
                                                                                                                                    aTGGCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTACGCAACATGGTggcg     >  1:309644/1‑150 (MQ=255)
                                                                                                                                       gCCGATCGGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCa  >  4:371840/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                              
GGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCA  >  NC_000913_3_bme_pgi/4158904‑4159188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑