Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 3,440,086 C→A G315V (GGC→GTC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi3,440,0860CA100.0% 191.5 / NA 56G315V (GGC→GTC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/28);  total (28/28)

AGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAG  >  NC_000913_3_bme_pgi/3439943‑3440230
                                                                                                                                               |                                                                                                                                                
ggCCTGGCGTTGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCagag                                                                                                                                            <  2:333207/149‑1 (MQ=255)
    tGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAg                                                                                                                                        >  2:354893/1‑150 (MQ=255)
     ggCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTCCTCAGTAAAACCTTAACCTGTGACCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCCTGACCAGAGACAGt                                                                                                                                       <  2:299086/150‑1 (MQ=255)
        gATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCa                                                                                                                                    <  2:309788/150‑1 (MQ=255)
              tgctgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGaa                                                                                                                              >  1:210991/1‑150 (MQ=255)
                        gTTCAGTTGACGACCACTCTTACGATGGCGCATGTCCTTATCCTTCACAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCTGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGt                                                                                                                    >  3:357670/1‑150 (MQ=255)
                          tCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCt                                                                                                                  >  1:438050/1‑150 (MQ=255)
                            aGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCtt                                                                                                                 >  2:288760/1‑149 (MQ=255)
                            aGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCtt                                                                                                                 <  1:288760/149‑1 (MQ=255)
                             gTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTa                                                                                                               >  3:336105/1‑150 (MQ=255)
                              ttGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCCGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCCGCGACAGTCCACCGCAAGCCAGCACCTCTTTac                                                                                                              >  2:370717/1‑149 (MQ=255)
                                   gacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAAACAGTCCACGGGAAGCCAGCAGGTCTTTAATCTCa                                                                                                         >  4:367766/1‑150 (MQ=255)
                                   gacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCCGTGATCCGGTTACTCGTCAGCGATGCTTGCAGTTGGCCAGTTTTCCAGGCCCATCACCATCGACAGTCCACGGGAAGCCAGCACGTATTTAATCTCa                                                                                                         <  2:322887/150‑1 (MQ=255)
                                       aCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTaa                                                                                                     <  1:296441/150‑1 (MQ=255)
                                              cGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAttt                                                                                              <  2:679946/150‑1 (MQ=255)
                                                aTGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa                                                                                                         <  1:705300/137‑1 (MQ=255)
                                                aTGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa                                                                                                         >  2:705300/1‑137 (MQ=255)
                                                            cTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCa                                                                                                                                               >  2:338354/1‑87 (MQ=255)
                                                            cTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCa                                                                                                                                               <  1:338354/87‑1 (MQ=255)
                                                                     cttaGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTaa                                                                       <  4:379097/147‑1 (MQ=255)
                                                                      tcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                      <  1:354893/150‑1 (MQ=255)
                                                                      tcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                      <  1:139188/150‑1 (MQ=255)
                                                                      ccaacaaaaCGTTAACCTGTGATCGGGTTACTCGGCAGCGATGCTTGCCGGTGGGCAGGTTTCCAGGCGCATTACTGGAGTGAGGCCACTGGAAGCCAGCGCGGCGTTGATCGCAGTAGGAGATTTTTTACCAATGGTAGGCGTTGTaat                                                                      <  4:371576/145‑2 (MQ=255)
                                                                         gTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATCTTTTACCAAGGTTAGGCGTTTTAAGGAg                                                                   <  4:42667/150‑1 (MQ=255)
                                                                          tAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTcc                                                                                                                                     >  1:570575/1‑83 (MQ=255)
                                                                          tAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTcc                                                                                                                                     <  2:570575/83‑1 (MQ=255)
                                                                          tAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGcc                                                                                                                           >  2:46065/1‑93 (MQ=255)
                                                                          tAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGcc                                                                                                                           <  1:46065/93‑1 (MQ=255)
                                                                          tAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAATGTTAGGCGTTTTAAGGAGc                                                                  >  1:359158/1‑150 (MQ=255)
                                                                           aaaaCCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCt                                                                 >  1:221657/1‑150 (MQ=255)
                                                                           aaaaCCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCt                                                                 >  1:221696/1‑150 (MQ=255)
                                                                                   aaCCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                         <  2:221657/150‑1 (MQ=255)
                                                                                   aaCCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                         <  2:221696/150‑1 (MQ=255)
                                                                                      cTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTa                                                      <  4:145079/150‑1 (MQ=255)
                                                                                       tgtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTAc                                                     >  2:686016/1‑150 (MQ=255)
                                                                                         tgATCCGGTTACTCGTCAGCGATGCATGCCGGCGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGc                                                   >  2:323824/1‑150 (MQ=255)
                                                                                          gATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAg                                                                   <  1:330714/133‑1 (MQ=255)
                                                                                          gATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAg                                                                   >  2:330714/1‑133 (MQ=255)
                                                                                              cGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTAc                                              <  4:336105/150‑1 (MQ=255)
                                                                                                      cGTCAGCGATGCTTGCCGGTGGCCTGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAc                                      >  4:116647/1‑150 (MQ=255)
                                                                                                      cGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGCAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAc                                      >  2:522847/1‑150 (MQ=255)
                                                                                                       gTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAcc                                     >  1:373146/1‑150 (MQ=255)
                                                                                                            cGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGatat                                >  4:224672/1‑150 (MQ=255)
                                                                                                                  ttGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCAc                                                                                                                                   >  4:173629/1‑45 (MQ=255)
                                                                                                                  ttGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCAc                                                                                                                                   <  3:173629/45‑1 (MQ=255)
                                                                                                                                     cAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAttttt                                                                                            >  1:173813/1‑65 (MQ=255)
                                                                                                                                     cAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAttttt                                                                                            <  2:173813/65‑1 (MQ=255)
                                                                                                                                     cAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTaa           >  1:393365/1‑146 (MQ=255)
                                                                                                                                     cAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTaa           <  2:393365/146‑1 (MQ=255)
                                                                                                                                      aGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGc                >  3:274902/1‑140 (MQ=255)
                                                                                                                                      aGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGc                <  4:274902/140‑1 (MQ=255)
                                                                                                                                      aGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAg      >  3:310855/1‑150 (MQ=255)
                                                                                                                                         cgcATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTa   <  2:607771/150‑1 (MQ=255)
                                                                                                                                          gcATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAg                              <  2:582954/122‑1 (MQ=255)
                                                                                                                                          gcATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAg                              >  1:582954/1‑122 (MQ=255)
                                                                                                                                          gcATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg  <  3:116647/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
AGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAG  >  NC_000913_3_bme_pgi/3439943‑3440230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑