Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	3,316,893	C→T	E212K (GAA→AAA)	nusA ←	transcription termination/antitermination L factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	3,316,893	0	C	T	100.0%	166.6 / NA	47	E212K (GAA→AAA)	nusA	transcription termination/antitermination L factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/24);  total (23/24)

TGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATATCTTCGCGC  >  NC_000913_3_bme_pgi/3316748‑3317038                                                                                                                                                  |                                                                                                                                                  tGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATa                                                                                                                                               >  3:156530/1‑150 (MQ=255)      acGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGaa                                                                                                                                          <  1:269526/150‑1 (MQ=255)           cgcCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGtt                                                                                                                                     <  4:156530/150‑1 (MQ=255)            gcCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAAt                                                                                                                                                <  1:41276/138‑1 (MQ=255)            gcCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAAt                                                                                                                                                >  2:41276/1‑138 (MQ=255)               aCGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGAt                                                                                                                                 >  3:106137/1‑150 (MQ=255)                cGCATACCTCCGCAAGCCCCCGCCGGTTCGATTCGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGCTCGCGAGCCGCTGCTTTAATTTCACTCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATc                                                                                                                                <  4:217501/150‑1 (MQ=255)                         aCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTcc                                                                                                                       <  4:206812/150‑1 (MQ=255)                         aCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTcc                                                                                                                       <  4:106137/150‑1 (MQ=255)                         aCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTcc                                                                                                                       >  1:97283/1‑150 (MQ=255)                            cAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGc                                                                                                                    <  1:259849/150‑1 (MQ=255)                             aaGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCt                                                                                                                   >  1:117043/1‑150 (MQ=255)                             aaGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCt                                                                                                                   >  3:312899/1‑150 (MQ=255)                             aaGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCt                                                                                                                   <  3:352831/150‑1 (MQ=255)                                   cTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGGTCGCGAGCCGCTGCTTTAATTTCAATCGCTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAAc                                                                                                             <  4:273865/150‑1 (MQ=255)                                   cTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAAc                                                                                                             >  3:273865/1‑150 (MQ=255)                                     aCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGa                                                                                                           <  3:218948/150‑1 (MQ=255)                                      ccGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAg                                                                                                          <  1:53198/150‑1 (MQ=255)                                      ccGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAg                                                                                                          <  2:235690/150‑1 (MQ=255)                                         gATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGa                                                                                                       <  2:117043/150‑1 (MQ=255)                                         gATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGa                                                                                                       >  1:530036/1‑150 (MQ=255)                                          aTCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGAc                                                                                                      <  3:273515/150‑1 (MQ=255)                                              aTACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAAc                                                                                                  >  2:526317/1‑150 (MQ=255)                                               tACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACa                                                                                                 <  4:170516/150‑1 (MQ=255)                                               tACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACa                                                                                                 >  1:570368/1‑150 (MQ=255)                                                aCGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAg                                                                                                <  3:291106/150‑1 (MQ=255)                                                   tttATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTg                                                                                             >  3:220247/1‑150 (MQ=255)                                                          ttGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCa                                                                                      >  1:196717/1‑150 (MQ=255)                                                            ggTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCAcg                                                                                    <  1:101171/150‑1 (MQ=255)                                                              ttttCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGACCAGTTCGATCAGCGTATGCGGTCTGGTGCTTGAAAAAAACAGATGCGCGCCAcgcg                                                                                  >  1:315048/1‑150 (MQ=255)                                                                      gcgATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATa                                                                                                                                               >  2:540677/1‑81 (MQ=255)                                                                      gcgATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATa                                                                                                                                               <  1:540677/81‑1 (MQ=255)                                                                               gCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACggca                                                                 >  4:77643/1‑148 (MQ=255)                                                                                        cccGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACg                                                        >  2:75873/1‑150 (MQ=255)                                                                                         ccGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATc                                                                                                                                <  3:2405/77‑1 (MQ=255)                                                                                         ccGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATc                                                                                                                                >  4:2405/1‑77 (MQ=255)                                                                                           ggATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCa                                                     >  1:191564/1‑150 (MQ=255)                                                                                                       gctgctTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCg                                         <  2:92352/150‑1 (MQ=255)                                                                                                                           ttcttcGCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCAcg                     >  2:456901/1‑150 (MQ=255)                                                                                                                                 gCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGgcag               <  3:233327/150‑1 (MQ=255)                                                                                                                                 gCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGgcag               <  2:530036/150‑1 (MQ=255)                                                                                                                                 gCCGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGgcag               >  1:262965/1‑150 (MQ=255)                                                                                                                                  ccGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAAcgcg                                             <  2:260344/119‑1 (MQ=255)                                                                                                                                  ccGATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAAcgcg                                             >  1:260344/1‑119 (MQ=255)                                                                                                                                    gATTTCTGGCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCat            >  4:231368/1‑150 (MQ=255)                                                                                                                                           ggCACTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATATCTTcg     <  1:456901/150‑1 (MQ=255)                                                                                                                                              aCTTTAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATATCTTCgcgc  >  2:567572/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                  TGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATATCTTCGCGC  >  NC_000913_3_bme_pgi/3316748‑3317038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑