Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,426,5930TA21.1% 84.9 / 21.1 38intergenic (+35/+36)rplI/yjfZ50S ribosomal subunit protein L9/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (15/15);  new base A (4/4);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCAC  >  NC_000913_3_bme_pgi/4426445‑4426736
                                                                                                                                                    |                                                                                                                                               
agCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCAtt                                                                                                                                                >  2:126332/1‑150 (MQ=255)
        tCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCg                                                                                                                                        <  2:50557/150‑1 (MQ=255)
          gTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCAATGGTCGGCGtt                                                                                                                                      <  3:67745/150‑1 (MQ=255)
               ccGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCt                                                                                                                                 <  3:189654/150‑1 (MQ=255)
                   aCGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCt                                                                                                                             <  3:71677/150‑1 (MQ=255)
                          tCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTc                                                                                                                      <  2:496520/150‑1 (MQ=255)
                                          gAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTc                                                                                                      <  1:314236/150‑1 (MQ=255)
                                              acGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGGGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCaa                                                                                                  >  4:160989/1‑150 (MQ=255)
                                                      aGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATcc                                                                                          >  4:356155/1‑150 (MQ=255)
                                                          tCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTc                                                                                      >  1:165667/1‑150 (MQ=255)
                                                               gTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCAATGGTCGGCg                                                                                                                                        >  1:576896/1‑95 (MQ=255)
                                                               gTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCAATGGTCGGCg                                                                                                                                        <  2:576896/95‑1 (MQ=255)
                                                                  tttcgCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATtct                                                                              >  4:201484/5‑150 (MQ=255)
                                                                     aGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCtt                                                                           >  2:388767/1‑150 (MQ=255)
                                                                                          aTCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCt                                                                                                                             >  1:292627/1‑79 (MQ=255)
                                                                                          aTCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCt                                                                                                                             <  2:292627/79‑1 (MQ=255)
                                                                                          aTCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCAtt                                                                                <  3:296957/124‑1 (MQ=255)
                                                                                          aTCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCAtt                                                                                >  4:296957/1‑124 (MQ=255)
                                                                                           tCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGttt                                                                                                                                     >  4:180668/1‑70 (MQ=255)
                                                                                           tCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGttt                                                                                                                                     <  3:180668/70‑1 (MQ=255)
                                                                                              tAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCa                                                             >  2:205012/1‑139 (MQ=255)
                                                                                              tAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCa                                                             <  1:205012/139‑1 (MQ=255)
                                                                                                      gtagCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTa                                          <  3:356155/150‑1 (MQ=255)
                                                                                                                 atTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGa                               <  1:126332/150‑1 (MQ=255)
                                                                                                                        aTTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTAttttt                                                                                                                       <  4:344783/55‑1 (MQ=255)
                                                                                                                        aTTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTAttttt                                                                                                                       >  3:344783/1‑55 (MQ=255)
                                                                                                                            aaCGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTTTTTTTTTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGAt                    >  4:43879/1‑150 (MQ=255)
                                                                                                                            aaCGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATgg                                                       >  1:158598/1‑115 (MQ=255)
                                                                                                                            aaCGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATgg                                                       <  2:158598/115‑1 (MQ=255)
                                                                                                                                agaCGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGtct                <  3:201484/150‑1 (MQ=255)
                                                                                                                                       aaaaCGCCGACCAATGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGc             <  4:121032/146‑1 (MQ=255)
                                                                                                                                       aaaaCGCCGACCAATGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGc             >  3:121032/1‑146 (MQ=255)
                                                                                                                                        aaaCGCCGACCAATGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCAtt                                                                                       >  2:566462/1‑71 (MQ=255)
                                                                                                                                        aaaCGCCGACCAATGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCAtt                                                                                       <  1:566462/71‑1 (MQ=255)
                                                                                                                                         aaCGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGc       >  3:181091/1‑150 (MQ=255)
                                                                                                                                           cGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCgg     <  1:388767/150‑1 (MQ=255)
                                                                                                                                            gCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTCGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGc    >  2:81772/1‑150 (MQ=255)
                                                                                                                                             ccGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCa   >  3:193470/1‑150 (MQ=255)
                                                                                                                                              cGACCAATGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCAc  >  1:8046/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
AGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCAC  >  NC_000913_3_bme_pgi/4426445‑4426736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑