Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,469,888 T→G 15.2% S290S (TCT→TCG atpD → F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,469,8880TG15.2% 172.0 / 33.2 79S290S (TCT→TCGatpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base T (43/24);  new base G (5/7);  total (48/31)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GTTATCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTT  >  Exported/2469818‑2469956
                                                                      |                                                                    
gTTATCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCt                                                                      <  1:2340304/71‑1 (MQ=255)
  tATCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTa                                                                     >  1:3183917/1‑70 (MQ=255)
   aTCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTAt                                                                    <  1:1744570/70‑1 (MQ=255)
   aTCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTAt                                                                    >  1:2043747/1‑70 (MQ=255)
    tCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCa                                                                  >  1:3226352/1‑71 (MQ=255)
     cAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCAc                                                                 >  1:3237604/1‑71 (MQ=255)
         cGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTcc                                                             >  1:3212824/1‑71 (MQ=255)
              cTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTa                                                                     <  1:2875385/58‑1 (MQ=255)
              cTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTAt                                                                    >  1:1567215/1‑59 (MQ=255)
              cTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTa                                                          >  1:1963583/1‑69 (MQ=255)
                ggCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATc                                                                   >  1:2466996/1‑58 (MQ=255)
                ggCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATc                                                                   >  1:2211391/1‑58 (MQ=255)
                ggCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATc                                                                   >  1:415046/1‑58 (MQ=255)
                   ggAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg                                                   <  1:1257744/71‑1 (MQ=255)
                    gAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg                                                   <  1:1887403/70‑1 (MQ=255)
                     aaGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGta                                                 <  1:861823/71‑1 (MQ=255)
                      agagATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGta                                                 <  1:1806405/70‑1 (MQ=255)
                       gagaTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCt                                                                      >  1:829358/1‑48 (MQ=255)
                       gagaTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCt                                                                      >  1:1956310/1‑48 (MQ=255)
                       gagaTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCt                                                                      >  1:1888344/1‑48 (MQ=255)
                          aTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACg                                             >  1:3367624/1‑70 (MQ=255)
                          aTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACg                                             >  1:2949495/1‑70 (MQ=255)
                          aTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACg                                             >  1:277513/1‑70 (MQ=255)
                              gCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACa                                                        >  1:2234281/1‑55 (MQ=255)
                              gCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACa                                                        >  1:2833313/1‑55 (MQ=255)
                              gCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACa                                                        >  1:98740/1‑55 (MQ=255)
                              gCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACa                                                        >  1:3231985/1‑55 (MQ=255)
                              gCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACa                                                        >  1:1205519/1‑55 (MQ=255)
                              gCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACa                                                        >  1:622869/1‑55 (MQ=255)
                               cGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCt                                        >  1:1155231/1‑70 (MQ=255)
                               cGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTg                                       >  1:2782294/1‑71 (MQ=255)
                                 ttCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCg                                     <  1:3401195/71‑1 (MQ=255)
                                 ttCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCg                                     <  1:3397186/71‑1 (MQ=255)
                                   cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg                                                   <  1:2051383/55‑1 (MQ=255)
                                   cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg                                                   <  1:1209989/55‑1 (MQ=255)
                                   cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg                                                   <  1:1734919/55‑1 (MQ=255)
                                   cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGa                                   >  1:2044478/1‑71 (MQ=255)
                                   cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGa                                   >  1:1775969/1‑71 (MQ=255)
                                     gCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGta                                                 >  1:3258571/1‑55 (MQ=255)
                                      cAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAt                                  <  1:602824/69‑1 (MQ=255)
                                         gAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgactt                             <  1:3045456/71‑1 (MQ=255)
                                         gAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgactt                             <  1:30089/71‑1 (MQ=255)
                                         gAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgactt                             <  1:1441752/71‑1 (MQ=255)
                                             gTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttgact                         <  1:1575890/71‑1 (MQ=255)
                                             gTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttga                           >  1:830049/1‑69 (MQ=255)
                                              tATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttgact                         <  1:2947086/70‑1 (MQ=255)
                                              tATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttgact                         <  1:2384686/70‑1 (MQ=255)
                                               aTCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTtgactga                       >  1:1691340/1‑71 (MQ=255)
                                                tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc                                          <  1:2837128/51‑3 (MQ=255)
                                                tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc                                          <  1:1522286/51‑3 (MQ=255)
                                                tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc                                          <  1:357078/51‑3 (MQ=255)
                                                tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc                                          <  1:2273389/51‑3 (MQ=255)
                                                tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc                                          <  1:2320244/51‑3 (MQ=255)
                                                tCACCTCCACCAAAACTGGTTCTATCACCTCCGTa                                                          <  1:2545540/35‑1 (MQ=255)
                                                tCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTtgactgac                      >  1:101238/1‑71 (MQ=255)
                                                 cACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGtat                                                >  1:1618831/1‑44 (MQ=255)
                                                 cACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGtat                                                >  1:3376782/1‑44 (MQ=255)
                                                 cACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGta                                                 >  1:1589875/1‑43 (MQ=255)
                                                 cACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGAcc                     >  1:904085/1‑71 (MQ=255)
                                                 cACCTCAACCAAAACTGGTTCTATCACCTCCGTACAGGCAGtat                                                >  1:1853/1‑44 (MQ=255)
                                                  aCCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGTTccc                                        >  1:807223/1‑50 (MQ=255)
                                                  aCCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGTTccc                                        >  1:1456628/1‑50 (MQ=255)
                                                  aCCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGTTccc                                        >  1:1656986/1‑50 (MQ=255)
                                                  aCCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGTTccc                                        >  1:1711370/1‑50 (MQ=255)
                                                  aCCTCCACCAAGAAAGGCTCGATCACCTCGAACCAGGCCGTATACGttc                                          >  1:230767/1‑47 (MQ=255)
                                                  aCCTCCACCAAAACTGGTTCTATCACCTCCGTACa                                                        >  1:902396/1‑35 (MQ=255)
                                                  aCCTCCACCAAAACTGGTTCTATCACCTCCGTACa                                                        >  1:1177211/1‑35 (MQ=255)
                                                   ccTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTAc                                          >  1:847435/1‑48 (MQ=255)
                                                   ccTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCg                   <  1:1487376/71‑1 (MQ=255)
                                                    cTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGt                  <  1:939294/71‑1 (MQ=255)
                                                       caccaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGtctc               >  1:2021175/1‑71 (MQ=255)
                                                       caccaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGtctc               >  1:521784/1‑71 (MQ=255)
                                                        accaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTcc              >  1:854831/1‑71 (MQ=255)
                                                        accaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTcc              >  1:1149309/1‑71 (MQ=255)
                                                        accaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTcc              >  1:1484170/1‑71 (MQ=255)
                                                           aaaaCTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCgg            >  1:2002623/1‑70 (MQ=255)
                                                           aaaaCTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGc           <  1:493031/71‑1 (MQ=255)
                                                             aaCTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttga                           >  1:56397/1‑53 (MQ=255)
                                                               ccggTTCGATCACCTCGATCCAGGCCGTCTACTTCCCTGCGGATGACTTGACCGACCCGTCGCCTGCAacc       <  1:308413/69‑1 (MQ=255)
                                                               ccggTTCGATCACCTCGATCCAGGCCGTCTACGTCCCTGCGGATGACTTGACCGACCCGTCGCCTGCAacc       <  1:2660082/69‑1 (MQ=255)
                                                                   ttCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCt   <  1:1712074/71‑1 (MQ=255)
                                                                   ttCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCt   <  1:2206227/71‑1 (MQ=255)
                                                                    tCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCtt  >  1:766038/1‑71 (MQ=255)
                                                                      |                                                                    
GTTATCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTT  >  Exported/2469818‑2469956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: