Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 204,556 A→G 100% intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported204,5560AG100.0% 173.1 / NA 53intergenic (+156/‑28)alaV/rrlHtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/20);  total (33/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGG  >  Exported/204492‑204610
                                                                |                                                      
aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                   <  1:628882/70‑1 (MQ=255)
 aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                   >  1:584478/1‑69 (MQ=255)
 aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                   >  1:843815/1‑69 (MQ=255)
   ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                                               <  1:88151/71‑1 (MQ=255)
          acTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCt                                        <  1:644245/71‑1 (MQ=255)
              aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc                                      <  1:1696861/69‑1 (MQ=255)
                 aatgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGtt                                 <  1:1100012/68‑1 (MQ=255)
                  atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg                                <  1:2858633/69‑1 (MQ=255)
                   tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                               <  1:2664495/70‑1 (MQ=19)
                   tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                               <  1:2650169/70‑1 (MQ=19)
                   tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                               <  1:2037128/70‑1 (MQ=19)
                    gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCa                                                  >  1:613811/1‑51 (MQ=16)
                    gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg                                    >  1:1952921/1‑65 (MQ=25)
                    gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg                                    >  1:860037/1‑65 (MQ=25)
                    gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg                                    >  1:460635/1‑65 (MQ=25)
                    gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg                                    >  1:2083452/1‑65 (MQ=25)
                    gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg                                    >  1:2084956/1‑65 (MQ=25)
                    gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg                                    >  1:3038096/1‑65 (MQ=25)
                      aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg                            >  1:1684708/1‑71 (MQ=21)
                        gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt                          <  1:1947470/71‑1 (MQ=21)
                             tCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                     >  1:2904320/1‑71 (MQ=21)
                                tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                                         >  1:3098343/1‑48 (MQ=255)
                                tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                               <  1:1210821/58‑1 (MQ=255)
                                  aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa                             >  1:1328766/1‑58 (MQ=255)
                                  aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa                             >  1:2382687/1‑58 (MQ=255)
                                  aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa                             >  1:988025/1‑58 (MQ=255)
                                  aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                 <  1:3369317/70‑1 (MQ=25)
                                  aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                 >  1:2611436/1‑70 (MQ=25)
                                   gTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg                                >  1:1168944/1‑54 (MQ=255)
                                   gTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg                                >  1:2572770/1‑54 (MQ=255)
                                   gTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg                                >  1:2130269/1‑54 (MQ=255)
                                      tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg            >  1:276918/1‑71 (MQ=21)
                                      tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg            >  1:225146/1‑71 (MQ=21)
                                       ctcAAATTTTCGCAACACGATGATGGATCGCAAGaa                                              <  1:371231/36‑1 (MQ=255)
                                       ctcAAATTTTCGCAACACGATGATGGATCGCAAGaa                                              <  1:2037129/36‑1 (MQ=255)
                                       ctcAAATTTTCGCAACACGATGATGGATCGCAAGa                                               <  1:3290883/35‑1 (MQ=255)
                                       ctcAAATTTTCGCAACACGATGATGGATCGCAAGa                                               <  1:2020453/35‑1 (MQ=255)
                                        tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa                             <  1:1586953/52‑1 (MQ=255)
                                        tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa          >  1:3422567/1‑71 (MQ=21)
                                         cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                              <  1:626362/50‑1 (MQ=255)
                                          aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg                    >  1:2597238/1‑59 (MQ=255)
                                          aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg                    >  1:2002123/1‑59 (MQ=255)
                                          aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa                   >  1:558953/1‑60 (MQ=255)
                                          aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa                   >  1:1841924/1‑60 (MQ=255)
                                          aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa                   >  1:829337/1‑60 (MQ=255)
                                          aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca        >  1:319047/1‑71 (MQ=21)
                                           aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAg              >  1:3404654/1‑64 (MQ=25)
                                           aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAg              >  1:3408588/1‑64 (MQ=25)
                                           aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAg              >  1:1841084/1‑64 (MQ=25)
                                             ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                               <  1:927816/45‑1 (MQ=255)
                                             ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg     >  1:1880007/1‑71 (MQ=21)
                                             ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg     >  1:1510335/1‑71 (MQ=21)
                                                 cGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTgg  <  1:1296310/70‑1 (MQ=25)
                                                            gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg            <  1:995071/49‑1 (MQ=255)
                                                            gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg            <  1:3315799/49‑1 (MQ=255)
                                                            gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg            <  1:26318/49‑1 (MQ=255)
                                                                |                                                      
AAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGG  >  Exported/204492‑204610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: