Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,644,441	T→A	6.8%	Q231H (CAA→CAT)	nlpB ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,644,441	0	T	A	6.8%	171.6 / 4.4	59	Q231H (CAA→CAT)	nlpB	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (23/32);  new base A (2/2);  total (25/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

GTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGGCAGATTTATCCAGAC  >  Exported/1644374‑1644511                                                                    |                                                                       gTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACtt                                                                        <  1:1476535/68‑1 (MQ=255) gTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTg                                                                      >  1:2769100/1‑70 (MQ=255)  ttGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTg                                                                      <  1:645629/69‑1 (MQ=255)  ttGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTg                                                                      <  1:140121/69‑1 (MQ=255)   tGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGGCATCAGCTGCACTTTg                                                                      <  1:930422/68‑1 (MQ=255)     ccAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGt                                                                 >  1:1025283/1‑71 (MQ=255)      cAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTc                                                                <  1:121824/71‑1 (MQ=255)       aaaCCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTc                                                                >  1:4098/1‑70 (MQ=255)        aaCCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCa                                                              >  1:1622030/1‑71 (MQ=255)        aaCCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCa                                                              <  1:1089862/71‑1 (MQ=255)           cacaTTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTATGGACGTACAAAg                                                           <  1:2475901/71‑1 (MQ=255)            acaTTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgt                                                          >  1:1096015/1‑71 (MQ=255)             caTTGAACGGCCCGCGTACGACCATCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtg                                                         <  1:215284/71‑1 (MQ=255)             caTTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtg                                                         <  1:282944/71‑1 (MQ=255)             caTTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtg                                                         <  1:974574/71‑1 (MQ=255)             caTTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtg                                                         <  1:2276920/71‑1 (MQ=255)              aTTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtgg                                                        >  1:1307264/1‑71 (MQ=255)              aTTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtgg                                                        >  1:1348224/1‑71 (MQ=255)              aTTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtg                                                         >  1:972442/1‑70 (MQ=255)                 gAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtggtg                                                      <  1:2561662/70‑1 (MQ=255)                  aaCGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtggtg                                                      >  1:2880668/1‑69 (MQ=255)                  aaCGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGtggtgg                                                     <  1:772668/70‑1 (MQ=255)                  aaCGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGtggtgg                                                     <  1:873049/70‑1 (MQ=255)                     ggCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGc                                                 <  1:2122714/71‑1 (MQ=255)                        ccGCGTACGACCAGCATTGTTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACg                                              <  1:123862/71‑1 (MQ=255)                         cgcgTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGa                                             <  1:813563/71‑1 (MQ=255)                         cgcgTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGa                                             <  1:2490558/71‑1 (MQ=255)                         cgcgTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGa                                             <  1:763239/71‑1 (MQ=255)                         cgcgTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGa                                             <  1:1491880/71‑1 (MQ=255)                           cgTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGAtt                                           >  1:897294/1‑71 (MQ=255)                              aCGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTg                                        >  1:2070982/1‑71 (MQ=255)                               cGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTgc                                       >  1:2999412/1‑71 (MQ=255)                               cGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTgc                                       >  1:1050543/1‑71 (MQ=255)                                  ccAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCa                                    <  1:26939/71‑1 (MQ=255)                                     gCATTGGTAAACCGGTGTCATCAGCTGCACTATGGACGTACAAAg                                                           <  1:2984836/45‑1 (MQ=25)                                      cATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtg                                                         <  1:3031863/46‑1 (MQ=255)                                       aTTGGTAAACCGGTGTCATCAGCTGCACTATGGACGTACAAAgtg                                                         >  1:2844247/1‑45 (MQ=21)                                       aTTGGTAAACCGGTGTCATCAGCTGCACTATGGACGTACAAAgtg                                                         >  1:418479/1‑45 (MQ=21)                                          ggTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtgg                                                        >  1:922105/1‑43 (MQ=255)                                          ggTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtgg                                                        >  1:1092739/1‑43 (MQ=255)                                          ggTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtgg                                                        >  1:576944/1‑43 (MQ=255)                                          ggTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTcg                             >  1:1415516/1‑70 (MQ=255)                                          ggTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGGGGAGGCACGATTTTGCGCAGCGTTcg                             <  1:697026/70‑1 (MQ=255)                                           gTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtggtg                                                      <  1:1300746/44‑1 (MQ=255)                                            tAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtggtg                                                      <  1:112498/43‑1 (MQ=255)                                             aaaCCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGa                                                    >  1:2216531/1‑44 (MQ=255)                                              aaCCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGa                                                    >  1:295652/1‑43 (MQ=255)                                                    tgtCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTg                  <  1:2380876/71‑1 (MQ=255)                                                     gtCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTgg                 >  1:2946515/1‑71 (MQ=255)                                                        atcaGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTc                              >  1:561064/1‑55 (MQ=255)                                                        atcaGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGGCAg              <  1:2787568/71‑1 (MQ=255)                                                             cTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGAGGCAGATTTa         <  1:985194/71‑1 (MQ=255)                                                               gCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGGCAGATTTATc       >  1:2568991/1‑71 (MQ=255)                                                                cACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGGCAGATTTATcc      <  1:1888968/71‑1 (MQ=255)                                                                cACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGGCAGATTTATcc      <  1:2946855/71‑1 (MQ=255)                                                                cACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGGCAGATTTATc       <  1:1106688/70‑1 (MQ=255)                                                                cACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGGCAGATTTATc       <  1:816481/70‑1 (MQ=255)                                                                 aCTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGGCAGATTTATCCa     <  1:602418/71‑1 (MQ=255)                                                                  cTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGGCAGATTTATc       >  1:989862/1‑68 (MQ=255)                                                                  cTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGGCAGATTTATCCAg    <  1:1655757/71‑1 (MQ=255)                                                                   tttGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTc                      <  1:3003637/52‑1 (MQ=255)                                                                    ttGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGc                                  >  1:2915630/1‑39 (MQ=255)                                                                    ttGTACATCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGGCAGATTTATCCAGAc  >  1:2474162/1‑71 (MQ=255)                                                                    |                                                                       GTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGGCAGATTTATCCAGAC  >  Exported/1644374‑1644511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑