Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 705,870 A→G 50.0% intergenic (‑367/+128) ymcC ← / ← serX predicted outer membrane lipoprotein/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported705,8700AG50.0% ‑1.4 / 21.6 16intergenic (‑367/+128)ymcC/serXpredicted outer membrane lipoprotein/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (6/2);  new base G (3/5);  total (9/7)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01

TCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCC  >  Exported/705844‑705939
                          |                                                                     
tcgctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAc                           >  1:2684389/1‑71 (MQ=32)
 cgctcgcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAcc                          >  1:1606757/1‑71 (MQ=21)
  gctcgcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAccc                         <  1:1087680/71‑1 (MQ=21)
  gctcgcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAccc                         <  1:785119/71‑1 (MQ=21)
    tcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCg                       >  1:1490232/1‑71 (MQ=34)
       cccTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTc                    <  1:1456230/71‑1 (MQ=21)
       cccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTc                    <  1:1882434/71‑1 (MQ=34)
         cTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCgg                  >  1:1487687/1‑71 (MQ=34)
            cGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGc               >  1:2031611/1‑71 (MQ=21)
            cGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCggg                 >  1:984778/1‑69 (MQ=35)
                cAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtct           <  1:169549/71‑1 (MQ=21)
                 aGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtctc          <  1:601969/71‑1 (MQ=21)
                   cccGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCAt        <  1:724774/71‑1 (MQ=34)
                    ccGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAcc                          >  1:67861/1‑52 (MQ=35)
                       cTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtctc          >  1:2259430/1‑65 (MQ=25)
                       cTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcccc    >  1:671873/1‑71 (MQ=34)
                          aCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcccccc  <  1:428107/70‑1 (MQ=35)
                          aCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcccccc  <  1:1051689/70‑1 (MQ=35)
                          |                                                                     
TCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCC  >  Exported/705844‑705939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: