Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 705,870 | A→G | 50.0% | intergenic (‑367/+128) | ymcC ← / ← serX | predicted outer membrane lipoprotein/tRNA‑Ser |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 705,870 | 0 | A | G | 50.0% | ‑1.4 / 21.6 | 16 | intergenic (‑367/+128) | ymcC/serX | predicted outer membrane lipoprotein/tRNA‑Ser |
Reads supporting (aligned to +/- strand): ref base A (6/2); new base G (3/5); total (9/7) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.15e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01 |
TCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCC > Exported/705844‑705939 | tcgctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAc > 1:2684389/1‑71 (MQ=32) cgctcgcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAcc > 1:1606757/1‑71 (MQ=21) gctcgcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAccc < 1:1087680/71‑1 (MQ=21) gctcgcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAccc < 1:785119/71‑1 (MQ=21) tcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCg > 1:1490232/1‑71 (MQ=34) cccTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTc < 1:1456230/71‑1 (MQ=21) cccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTc < 1:1882434/71‑1 (MQ=34) cTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCgg > 1:1487687/1‑71 (MQ=34) cGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGc > 1:2031611/1‑71 (MQ=21) cGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCggg > 1:984778/1‑69 (MQ=35) cAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtct < 1:169549/71‑1 (MQ=21) aGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtctc < 1:601969/71‑1 (MQ=21) cccGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCAt < 1:724774/71‑1 (MQ=34) ccGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAcc > 1:67861/1‑52 (MQ=35) cTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtctc > 1:2259430/1‑65 (MQ=25) cTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcccc > 1:671873/1‑71 (MQ=34) aCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcccccc < 1:428107/70‑1 (MQ=35) aCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcccccc < 1:1051689/70‑1 (MQ=35) | TCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCC > Exported/705844‑705939 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |