Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,763,580 G→T 6.2% noncoding (264/1542 nt) rrsG ← 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,763,5800GT 6.2% 272.0 / 2.3 79noncoding (264/1542 nt)rrsG16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (28/46);  new base T (3/2);  total (31/48)
Fisher's exact test for biased strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTG  >  Exported/1763511‑1763650
                                                                     |                                                                      
aCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGt                                                                       <  1:2291173/71‑1 (MQ=34)
aCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGt                                                                       <  1:92239/71‑1 (MQ=34)
 ccGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGtt                                                                      <  1:322810/71‑1 (MQ=34)
 ccGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGt                                                                       >  1:34137/1‑70 (MQ=35)
 ccGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGt                                                                       >  1:725313/1‑70 (MQ=35)
  cGTGTCTCAGTTCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTa                                                                     >  1:2234487/1‑71 (MQ=11)
  cGTGTCTCAGTTCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTa                                                                     >  1:2064553/1‑71 (MQ=11)
   gtgtCTCAGTTCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTAc                                                                    >  1:2812637/1‑71 (MQ=11)
     gtCTCAGTTCCAGTGTGGCTGGTCATCCTCTCATACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTAc                                                                    <  1:629313/69‑1 (MQ=255)
     gtCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAccc                                                                  >  1:117713/1‑71 (MQ=34)
      tctcAGTTCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTAcctc                                                                 <  1:2114044/71‑3 (MQ=11)
      tctcAGTTCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTAcctc                                                                 <  1:1445443/71‑3 (MQ=11)
      tctcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGt                                                                       <  1:1206147/65‑1 (MQ=35)
      tctcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAccc                                                                  <  1:2317573/70‑1 (MQ=35)
        tcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccac                                                               >  1:1439129/1‑71 (MQ=21)
          aGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                                                             <  1:805551/71‑1 (MQ=35)
          aGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                                                             <  1:2998771/71‑1 (MQ=35)
          aGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCccacc                                                              >  1:2588559/1‑70 (MQ=35)
            ttCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                                           <  1:1069538/71‑1 (MQ=39)
             tCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                                           >  1:553835/1‑70 (MQ=255)
              ccAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaaca                                                          <  1:1426133/70‑1 (MQ=255)
              ccAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaaca                                                          <  1:2970047/70‑1 (MQ=255)
              ccAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                                           >  1:2829712/1‑69 (MQ=40)
              ccAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                                           >  1:708595/1‑69 (MQ=40)
               cAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAg                                                        <  1:2579144/71‑1 (MQ=255)
               cAGTGTGGCTGGTCATCCTCCCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaacaa                                                         <  1:1201307/70‑1 (MQ=255)
                aGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAg                                                        <  1:1998922/70‑1 (MQ=255)
                aGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaacaa                                                         <  1:2043415/69‑1 (MQ=255)
                   gtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                                                    >  1:2376979/1‑71 (MQ=255)
                   gtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGAGAGCCGTTACCCCACCAACAAGCTaa                                                    >  1:857008/1‑71 (MQ=255)
                    tgGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                                                    <  1:972724/70‑1 (MQ=255)
                      gCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATcc                                                 >  1:1797169/1‑71 (MQ=255)
                       cTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATcc                                                 >  1:2771500/1‑70 (MQ=255)
                         ggTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                                                    <  1:1307396/65‑1 (MQ=255)
                          gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                                             <  1:112475/71‑1 (MQ=255)
                          gTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCAt                                              <  1:1798062/70‑1 (MQ=255)
                          gTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCAt                                              <  1:766141/70‑1 (MQ=255)
                             aTCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTgg                                          >  1:236996/1‑71 (MQ=255)
                                ctctCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGca                                       >  1:2567421/1‑71 (MQ=255)
                                ctctCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTa                                                                     >  1:2310381/1‑41 (MQ=255)
                                ctctCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTa                                                                     >  1:1407705/1‑41 (MQ=255)
                                 tctcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTggg                                         >  1:148330/1‑68 (MQ=255)
                                 tctcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGca                                       >  1:1789183/1‑70 (MQ=255)
                                  ctcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                                                     >  1:1160361/1‑55 (MQ=255)
                                  ctcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTa                                                     >  1:1535652/1‑55 (MQ=255)
                                   tcAGACCAGCTAGGGATCGACGCCTTGGTGAGCCGTTa                                                                     >  1:2522269/1‑38 (MQ=255)
                                     aGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                  <  1:1231157/71‑1 (MQ=255)
                                     aGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                  <  1:677553/71‑1 (MQ=255)
                                     aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACAt                                    <  1:828174/69‑1 (MQ=255)
                                     aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                  <  1:1356616/71‑1 (MQ=255)
                                     aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc                                  >  1:2513018/1‑71 (MQ=255)
                                      gACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCg                                 <  1:521077/71‑1 (MQ=255)
                                       aCCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGa                                <  1:928956/71‑1 (MQ=255)
                                       aCCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGa                                <  1:915099/71‑1 (MQ=255)
                                         cAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCa                                               >  1:1707202/1‑54 (MQ=255)
                                         cAGCTAGGGATCGTCCCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCa                                               >  1:303607/1‑54 (MQ=255)
                                           gCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGc                            <  1:388795/71‑1 (MQ=255)
                                             caGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCaa                          <  1:1840125/70‑1 (MQ=255)
                                                ggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCg                                 <  1:2108278/61‑1 (MQ=255)
                                                ggATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCg                                 <  1:1403563/61‑1 (MQ=255)
                                                    agtcgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGccc                   >  1:1482084/2‑71 (MQ=255)
                                                       cgCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCg                  >  1:857574/1‑69 (MQ=255)
                                                         ccTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCaa                          <  1:645177/59‑1 (MQ=255)
                                                         ccTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCg                  <  1:445101/67‑1 (MQ=255)
                                                         ccTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAggg              <  1:2751618/71‑1 (MQ=255)
                                                          cTTGGTGAGCCGTTACCCCACCAACAAGCTAATcc                                                 <  1:326226/35‑1 (MQ=255)
                                                          cTTGGTGAGCCGTTACCCCACCAACAAGCTAATcc                                                 <  1:1360436/35‑1 (MQ=255)
                                                            tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCt                                            <  1:1499890/38‑1 (MQ=255)
                                                             ggTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTc            <  1:2588313/69‑1 (MQ=255)
                                                                gAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGa                                >  1:2159488/1‑46 (MQ=255)
                                                                gAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAag                         <  1:1060074/53‑1 (MQ=255)
                                                                  gCCGTTACCCCACCAACAAGCTAATCCCATCTGGGc                                        <  1:1872561/36‑1 (MQ=255)
                                                                  gCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCctct     <  1:1006842/71‑1 (MQ=255)
                                                                  gCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCctct     <  1:1010439/71‑1 (MQ=255)
                                                                  gCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCctct     <  1:1499787/71‑1 (MQ=255)
                                                                  gCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct     <  1:752786/71‑1 (MQ=39)
                                                                   ccGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAggg              <  1:248207/61‑1 (MQ=255)
                                                                   ccGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCtt    >  1:2297303/1‑71 (MQ=39)
                                                                    cGTTACCCCACCAACAAGCTAATCCCATCTGGGcaca                                     <  1:2205481/37‑1 (MQ=255)
                                                                     gTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTg  <  1:1405403/71‑1 (MQ=39)
                                                                     |                                                                      
ACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTG  >  Exported/1763511‑1763650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑