| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,123 | C→G | 100% | R159R (CGC→CGG) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,123 | 0 | C | G | 100.0% | 233.9 / ‑6.7 | 68 | R159R (CGC→CGG) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (30/37); minor base T (0/1); total (30/38) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
TGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCA > AP009048/3825056‑3825190 | tGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGgggg < 1:664616/71‑1 (MQ=255) gagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcg < 1:3523770/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTTGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:2299867/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:2872675/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:1437815/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:818771/71‑1 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:1477384/1‑71 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc < 1:3381109/71‑1 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcgg < 1:3169786/70‑1 (MQ=255) tattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:1422559/1‑70 (MQ=255) tattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:3994035/1‑70 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:1515632/1‑68 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCgag < 1:45608/71‑1 (MQ=255) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcg > 1:1185276/1‑61 (MQ=255) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAtt < 1:515600/71‑1 (MQ=255) ggCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:2790021/71‑1 (MQ=255) ctaaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCg < 1:521735/69‑1 (MQ=255) aTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAtt > 1:3967269/1‑65 (MQ=255) aTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCgg < 1:1791900/69‑1 (MQ=255) tGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGgggg < 1:385098/52‑1 (MQ=255) tGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGTGGGCGCGGCGAGATTTCGGCg < 1:1652882/70‑1 (MQ=255) tGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGa > 1:1507987/1‑71 (MQ=255) tGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGCGCGCGGCGAGATTTCGGCGa > 1:103649/1‑71 (MQ=255) ggCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAt > 1:4317804/1‑71 (MQ=255) ggCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAt > 1:2012385/1‑71 (MQ=255) gcgcGTCGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt < 1:1991209/71‑1 (MQ=255) gcgcGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt > 1:343675/1‑71 (MQ=255) gACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg < 1:5249711/71‑1 (MQ=255) gACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg < 1:3642596/71‑1 (MQ=255) gACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg < 1:3307753/71‑1 (MQ=255) aCGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCga < 1:2857306/53‑1 (MQ=255) aCGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCg < 1:4466852/60‑1 (MQ=255) aCGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg > 1:803068/1‑70 (MQ=255) aCGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg > 1:809314/1‑70 (MQ=255) cGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg > 1:2926667/1‑69 (MQ=255) gCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAg > 1:2593382/1‑71 (MQ=255) tGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGtt > 1:4086254/1‑70 (MQ=255) gCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGtt < 1:4241194/69‑1 (MQ=255) gCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGtt < 1:4561962/69‑1 (MQ=255) cTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCga < 1:44209/46‑1 (MQ=38) tGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:2678767/41‑1 (MQ=37) tGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAg < 1:1654460/65‑1 (MQ=255) gCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGt < 1:3075122/71‑1 (MQ=255) gCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGt > 1:1835720/1‑71 (MQ=255) attGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGt < 1:4882202/66‑1 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTg < 1:3523858/61‑1 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa > 1:861699/1‑71 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa > 1:4895025/1‑71 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa > 1:1903922/1‑71 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa > 1:2742988/1‑71 (MQ=255) tGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa > 1:4395987/1‑70 (MQ=255) gATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGtt > 1:3177528/1‑58 (MQ=255) gATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTAc < 1:850844/70‑1 (MQ=255) aTTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGAtt < 1:2452891/67‑1 (MQ=255) aTTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACaa < 1:4291460/71‑1 (MQ=255) ttCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACt > 1:449507/1‑71 (MQ=255) gCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGc > 1:28862/1‑70 (MQ=255) gCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCa < 1:922962/71‑1 (MQ=255) tgATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAg > 1:4252781/2‑71 (MQ=255) tgATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAg > 1:1576985/2‑71 (MQ=255) gATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCg < 1:2287662/36‑1 (MQ=255) gATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCaaa < 1:4586538/69‑1 (MQ=255) aTCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGt < 1:4165249/70‑1 (MQ=255) gTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTAc < 1:548035/56‑1 (MQ=255) aGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGa > 1:197695/1‑37 (MQ=255) ggAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGAtt > 1:31329/1‑50 (MQ=255) gAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGtt < 1:1461419/39‑1 (MQ=255) aaCGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACaa < 1:2574390/52‑1 (MQ=255) aCGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTatca > 1:448517/1‑71 (MQ=255) aCGGGGGCGCGGAGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTatca < 1:759185/71‑1 (MQ=255) | TGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCA > AP009048/3825056‑3825190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |