| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,108 | C→T | 100% | L154L (CTC→CTT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,108 | 0 | C | T | 100.0% | 249.0 / NA | 83 | L154L (CTC→CTT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (35/48); total (35/48) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCA > AP009048/3825040‑3825175 | gCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGa < 1:617120/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt > 1:4122728/1‑71 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:2572157/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:2181241/71‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt > 1:2429780/1‑70 (MQ=255) cgcgATTCGATGGAGATTATTCAGGCTTATGGCGCGTCGCTTGCTGGCGTATTGATTTCGCTTGATCGTc < 1:5207062/70‑1 (MQ=255) gcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg > 1:1929920/1‑71 (MQ=255) cgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAgg < 1:5131789/71‑1 (MQ=255) cgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAgg < 1:1031153/71‑1 (MQ=255) gaatCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGa < 1:4606113/68‑1 (MQ=255) gaatCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGa < 1:5022526/68‑1 (MQ=255) atCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAc < 1:281851/70‑1 (MQ=255) atCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAc < 1:2496723/70‑1 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcg > 1:3432087/1‑71 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACTCTTGCTGGCGTATTGATTTCGCTTGATCGTCa > 1:39516/1‑63 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg > 1:1681475/1‑69 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg < 1:2709218/71‑3 (MQ=255) tGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg > 1:1448637/1‑62 (MQ=255) tGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGgggg < 1:664616/71‑1 (MQ=255) gagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcg < 1:3523770/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTTGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:2299867/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGaa < 1:2286496/61‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg > 1:875163/1‑63 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:2872675/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:1437815/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:818771/71‑1 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:1477384/1‑71 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc < 1:3381109/71‑1 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcgg < 1:3169786/70‑1 (MQ=255) tattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:3994035/1‑70 (MQ=255) tattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:1422559/1‑70 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:1515632/1‑68 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCgag < 1:45608/71‑1 (MQ=255) tCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:325361/49‑1 (MQ=38) cAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:1360805/48‑1 (MQ=38) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcg > 1:1185276/1‑61 (MQ=255) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAtt < 1:515600/71‑1 (MQ=255) ggCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:2790021/71‑1 (MQ=255) ctaaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCg < 1:521735/69‑1 (MQ=255) aaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:5361619/1‑39 (MQ=37) aTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAtt > 1:3967269/1‑65 (MQ=255) aTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCgg < 1:1791900/69‑1 (MQ=255) tGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcgg < 1:2773914/49‑2 (MQ=38) tGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGgggg < 1:385098/52‑1 (MQ=255) tGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGTGGGCGCGGCGAGATTTCGGCg < 1:1652882/70‑1 (MQ=255) tGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGa > 1:1507987/1‑71 (MQ=255) tGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGCGCGCGGCGAGATTTCGGCGa > 1:103649/1‑71 (MQ=255) ggCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAt > 1:2012385/1‑71 (MQ=255) ggCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAt > 1:4317804/1‑71 (MQ=255) gcgcGTCGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt < 1:1991209/71‑1 (MQ=255) gcgcGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt > 1:343675/1‑71 (MQ=255) gACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg < 1:5249711/71‑1 (MQ=255) gACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg < 1:3307753/71‑1 (MQ=255) gACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg < 1:3642596/71‑1 (MQ=255) aCGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCga < 1:2857306/53‑1 (MQ=255) aCGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCg < 1:4466852/60‑1 (MQ=255) aCGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg > 1:809314/1‑70 (MQ=255) aCGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg > 1:803068/1‑70 (MQ=255) cGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg > 1:2926667/1‑69 (MQ=255) gCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAg > 1:2593382/1‑71 (MQ=255) tGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGtt > 1:4086254/1‑70 (MQ=255) gCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGtt < 1:4241194/69‑1 (MQ=255) gCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGtt < 1:4561962/69‑1 (MQ=255) cTGGCGTATTGATTTCGCTTGATCGTCAGGAAcgg > 1:4007665/1‑34 (MQ=37) cTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCga < 1:44209/46‑1 (MQ=38) tGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:2678767/41‑1 (MQ=37) tGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAg < 1:1654460/65‑1 (MQ=255) gCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGt > 1:1835720/1‑71 (MQ=255) gCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGt < 1:3075122/71‑1 (MQ=255) attGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGt < 1:4882202/66‑1 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTg < 1:3523858/61‑1 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa > 1:2742988/1‑71 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa > 1:1903922/1‑71 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa > 1:4895025/1‑71 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa > 1:861699/1‑71 (MQ=255) tGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa > 1:4395987/1‑70 (MQ=255) gATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGtt > 1:3177528/1‑58 (MQ=255) gATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTAc < 1:850844/70‑1 (MQ=255) aTTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGAtt < 1:2452891/67‑1 (MQ=255) aTTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACaa < 1:4291460/71‑1 (MQ=255) ttCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACt > 1:449507/1‑71 (MQ=255) gCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGc > 1:28862/1‑70 (MQ=255) gCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCa < 1:922962/71‑1 (MQ=255) | GCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCA > AP009048/3825040‑3825175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |