| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,051 | G→A | 100% | E135E (GAG→GAA) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,051 | 0 | G | A | 100.0% | 290.6 / ‑6.7 | 96 | E135E (GAG→GAA) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (36/59); minor base T (0/1); total (36/60) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGG > AP009048/3824984‑3825118 | gtAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATc < 1:874121/70‑1 (MQ=255)gtAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATc < 1:655465/70‑1 (MQ=255)gtAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATc < 1:2199552/70‑1 (MQ=255)gtAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCg < 1:1245477/71‑1 (MQ=255) tAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGa < 1:3401944/71‑1 (MQ=255) tAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGa > 1:4910867/1‑71 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt < 1:1552183/70‑1 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg > 1:26930/1‑71 (MQ=255) gcgTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg > 1:1773447/1‑69 (MQ=255) gcgTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg > 1:1767016/1‑69 (MQ=255) gTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg > 1:3578126/1‑67 (MQ=255) cAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGACCGGCGATTCGCGAATCGATGGAGattat < 1:213938/71‑1 (MQ=255) cAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattat < 1:4008054/71‑1 (MQ=255) aaGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattatt < 1:752941/71‑1 (MQ=255) aGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTc < 1:3262245/71‑1 (MQ=255) ggACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCa < 1:1339998/71‑1 (MQ=255) gACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAg < 1:5373618/71‑1 (MQ=255) aCGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGatta < 1:1522961/65‑1 (MQ=255) cgcgTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGc < 1:1960952/71‑1 (MQ=255) cgTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGcta < 1:1155042/71‑3 (MQ=255) gTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa < 1:2379423/71‑1 (MQ=255) gTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:2175215/1‑71 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt > 1:4338353/1‑71 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt < 1:944384/71‑1 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATc > 1:5219463/1‑47 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattatt > 1:3692528/1‑60 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg > 1:442658/1‑71 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg < 1:1754895/71‑1 (MQ=255) aTGCTGGTAGATGATGTGATCACAGCCGGAACGGCGATTCGCGAAACGATGGAGATTATTCAGGcta > 1:4782527/1‑65 (MQ=255) tGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt < 1:5266019/49‑1 (MQ=255) tGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg < 1:209060/69‑1 (MQ=255) tGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg < 1:1144436/69‑1 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:2415777/71‑1 (MQ=255) gTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGgaga > 1:2845795/1‑49 (MQ=255) tAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcg < 1:452084/66‑1 (MQ=255) tAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcg < 1:386110/68‑1 (MQ=255) tAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAAGGAGATTATTCAGGct < 1:3680219/59‑2 (MQ=255) aGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGgag > 1:5312397/1‑46 (MQ=255) gatgatGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCt > 1:3187031/1‑71 (MQ=255) atgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:3146230/64‑1 (MQ=255) atgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCtt > 1:4978972/1‑71 (MQ=255) atgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:5218356/61‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAg > 1:2906386/1‑49 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg < 1:5202710/71‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg < 1:1082817/71‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg < 1:4679733/71‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg < 1:31232/71‑1 (MQ=255) gtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTgg > 1:4114370/1‑71 (MQ=255) tgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGa > 1:4567979/1‑60 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATgg < 1:1107762/35‑1 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGc < 1:1078146/48‑1 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg > 1:5218470/1‑71 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg > 1:4652005/1‑71 (MQ=255) aTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg < 1:2105918/70‑1 (MQ=255) aTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg > 1:1875520/1‑70 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta > 1:4874633/1‑70 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta < 1:1230363/71‑2 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:2783251/1‑69 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:2538699/1‑69 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:1948340/1‑69 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:742818/1‑69 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat < 1:396331/70‑3 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTAtt < 1:2177988/71‑1 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTAtt < 1:2142433/71‑1 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTg > 1:4917043/1‑70 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGa > 1:2043124/1‑71 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGa < 1:729446/71‑1 (MQ=255) gccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAgg > 1:2364082/1‑40 (MQ=255) gccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCt < 1:2789603/60‑1 (MQ=255) gccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAt < 1:1516390/71‑1 (MQ=255) gccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAt < 1:1471559/71‑1 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGc > 1:958458/1‑54 (MQ=39) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt > 1:5151739/1‑71 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:2972446/71‑1 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:1994214/71‑1 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:217157/71‑1 (MQ=255) cgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg < 1:2567044/45‑1 (MQ=38) ggAACGGCGATTCGCGAATCGATCGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc < 1:3502057/71‑1 (MQ=255) gAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc < 1:1643196/70‑1 (MQ=255) aaCGGCGATTCGCGAATCGATGGATATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:2550445/71‑1 (MQ=255) cGGCGATTCGCGAATCGATGGAGATTATTCAGGct < 1:4717599/35‑2 (MQ=37) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:1896724/1‑37 (MQ=37) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:5296478/43‑1 (MQ=38) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGc < 1:3931762/56‑1 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:1073394/71‑3 (MQ=255) gCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGa < 1:617120/71‑1 (MQ=255) aTTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTg < 1:4437258/46‑1 (MQ=38) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:2572157/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:2181241/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt > 1:4122728/1‑71 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt > 1:263286/1‑58 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt > 1:2429780/1‑70 (MQ=255) cgcgATTCGATGGAGATTATTCAGGCTTATGGCGCGTCGCTTGCTGGCGTATTGATTTCGCTTGATCGTc < 1:5207062/70‑1 (MQ=255) gcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg > 1:1929920/1‑71 (MQ=255) cgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAgg < 1:1031153/71‑1 (MQ=255) cgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAgg < 1:5131789/71‑1 (MQ=255) | GTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGG > AP009048/3824984‑3825118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |