Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,684,858 A→G 100% pseudogene (201/582 nt)
pseudogene (1264/1645 nt)
ilvG ←
ilvG ←
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,684,8580AG100.0% 128.3 / NA 37pseudogene (201/582 nt)
pseudogene (1264/1645 nt)
ilvG
ilvG
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/25);  total (12/25)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTTTACGGTGCCCAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACC  >  AP009048/3684797‑3684923
                                                             |                                                                 
gTTTTACGGTGCCCAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCg                                                          >  1:41612/1‑71 (MQ=255)
 ttttACGGTGCCCAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGt                                                         <  1:2191685/71‑1 (MQ=255)
       ggTGCCCAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGtttg                                                      <  1:2878790/68‑3 (MQ=255)
       ggTGCCCAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTgg                                                     <  1:1741293/69‑1 (MQ=255)
         tGCCCAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTcgc                                                 <  1:3079662/71‑1 (MQ=255)
              aGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGTTATCGTTTGGTCGCGCGAc                                            <  1:2551685/71‑1 (MQ=255)
              aGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGAc                                            >  1:1296544/1‑71 (MQ=255)
              aGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGAc                                            <  1:1499237/71‑1 (MQ=255)
                   ttGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTtg                                         <  1:493307/69‑1 (MQ=255)
                    tGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTcgc                                                 <  1:2307300/60‑1 (MQ=255)
                    tGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTcg                                                  <  1:293479/59‑1 (MQ=255)
                     gCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTgcgc                                      >  1:3213881/1‑70 (MQ=255)
                     gCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGcgcg                                     >  1:3289431/1‑71 (MQ=255)
                        caTTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGt                                                         <  1:3384445/48‑1 (MQ=255)
                        caTTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTgcgcgc                                    >  1:522398/1‑69 (MQ=255)
                           tcatcaTGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTcgcgcg                                              >  1:3109925/1‑56 (MQ=255)
                            catcatGAAAGAGCCGTCACCGGAGATACAgacgac                                                                 >  1:1249703/1‑36 (MQ=255)
                            catcatGAAAGAGCCGTCACCGGAGATACAgacgac                                                                 >  1:3255235/1‑36 (MQ=255)
                              tcatGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGAc                                            <  1:1616307/55‑1 (MQ=255)
                               catGAAAGAGCCGTCACCGGAGATACACACGACGGTATCGTTTGGTcgc                                                 <  1:1166809/49‑1 (MQ=38)
                                  gAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACt                                           >  1:3536988/1‑52 (MQ=255)
                                    aaGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTtgcg                                       <  1:966728/54‑3 (MQ=255)
                                    aaGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTtgc                                        <  1:2443583/53‑2 (MQ=255)
                                     agagCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTtg                                         <  1:3355208/51‑1 (MQ=255)
                                       agCCGTCACCGGAGATACAGACGACGGTATCGTTTGGGcgcgcg                                              <  1:361811/44‑1 (MQ=255)
                                         ccGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTtg                                         <  1:2952770/47‑1 (MQ=255)
                                         ccGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCgg                       <  1:2870956/65‑1 (MQ=255)
                                          cGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGAc                                            <  1:1412327/43‑1 (MQ=38)
                                          cGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAccgcc                            <  1:609841/59‑1 (MQ=255)
                                             cACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCaaaa             >  1:4293296/1‑71 (MQ=255)
                                              aCCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAAc            >  1:699388/1‑71 (MQ=255)
                                               ccGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAAcc           >  1:1242849/1‑71 (MQ=255)
                                                cGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAAcc           >  1:1979605/1‑70 (MQ=255)
                                                    gaTACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGcc                                   <  1:802628/42‑1 (MQ=37)
                                                      tACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTaaa                   <  1:1181758/56‑1 (MQ=255)
                                                      aaCAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCaa               <  1:573832/59‑1 (MQ=255)
                                                        cAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGcc  >  1:4090211/1‑71 (MQ=38)
                                                         aGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAcc                               <  1:1784896/41‑1 (MQ=37)
                                                         aGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCa                <  1:5202355/56‑1 (MQ=255)
                                                             |                                                                 
GTTTTACGGTGCCCAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACC  >  AP009048/3684797‑3684923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: