Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,684,717 A→G 100% pseudogene (342/582 nt)
pseudogene (1405/1645 nt)
ilvG ←
ilvG ←
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,684,7170AG100.0% 349.9 / NA 98pseudogene (342/582 nt)
pseudogene (1405/1645 nt)
ilvG
ilvG
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/52);  total (46/52)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 108 total aligned reads displayed.

CGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCAGTAAGGGTGGTTTCGCTGTATCGTTCCTGAAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAAC  >  AP009048/3684650‑3684783
                                                                   |                                                                  
 gAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaa                                                                 >  1:2884591/1‑70 (MQ=255)
  aaGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                                               >  1:1119941/1‑71 (MQ=255)
  aaGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                                               >  1:4615428/1‑71 (MQ=255)
    ggCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaa                                                                <  1:3073678/68‑1 (MQ=255)
     gcgcTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAg                                                            <  1:2823312/71‑1 (MQ=255)
     gcgcTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAg                                                            <  1:1793399/71‑1 (MQ=255)
      cgcTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGt                                                           <  1:72082/71‑1 (MQ=255)
        cTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtg                                                         <  1:5138101/71‑1 (MQ=255)
         tGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgc                                                        >  1:5197318/1‑71 (MQ=255)
         tGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgc                                                        >  1:3948820/1‑71 (MQ=255)
           gCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgctg                                                      >  1:4544153/1‑71 (MQ=255)
             tAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGtt                                                          >  1:4101208/1‑65 (MQ=255)
              aaCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAAc                                                              <  1:3773027/60‑1 (MQ=255)
              aaCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGt                                                           <  1:669714/63‑1 (MQ=255)
              aaCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgctg                                                      >  1:419031/1‑68 (MQ=255)
               aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGtt                                                          <  1:107172/63‑1 (MQ=255)
               aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgctg                                                      >  1:4727162/1‑67 (MQ=255)
               aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCa                                                   <  1:3088751/70‑1 (MQ=255)
               aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCAt                                                  >  1:4272919/1‑71 (MQ=255)
               aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCAt                                                  >  1:3785174/1‑71 (MQ=255)
               aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATAGTTCCTGGAAAAACAGTTGCTGCCa                                                   <  1:856777/70‑1 (MQ=255)
                cATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCa                                                   <  1:2525663/69‑1 (MQ=255)
                cATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCAtt                                                 <  1:865326/71‑1 (MQ=255)
                 aTGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTg                                                <  1:2564019/71‑1 (MQ=255)
                 aTGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTg                                                >  1:1631395/1‑71 (MQ=255)
                   gAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                                               >  1:2280790/1‑54 (MQ=255)
                   gAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgc                                                        <  1:4252661/61‑1 (MQ=255)
                   gAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATAACGTTCCTGgaaaa                                                                >  1:3477294/1‑53 (MQ=255)
                    aGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                             <  1:820412/71‑1 (MQ=255)
                    aGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                             <  1:4801936/71‑1 (MQ=255)
                     ggAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                             <  1:1479771/70‑1 (MQ=255)
                     ggAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                             >  1:3200681/1‑70 (MQ=255)
                          tCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTACTGCCATTGTCGAACCAt                                       <  1:4864395/71‑1 (MQ=255)
                           cGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATc                                      >  1:3879371/1‑71 (MQ=255)
                             gggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATcc                                     >  1:38688/1‑70 (MQ=255)
                              ggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCt                                   <  1:4541098/71‑1 (MQ=255)
                              ggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCt                                   <  1:735323/71‑1 (MQ=255)
                                ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                                               <  1:1900458/41‑1 (MQ=255)
                                ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                                               <  1:414901/41‑1 (MQ=255)
                                ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaa                                                                <  1:5034384/40‑1 (MQ=255)
                                ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgct                                                       >  1:4563491/1‑49 (MQ=38)
                                 tATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAAc                                >  1:176369/1‑71 (MQ=255)
                                   tcggTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAAcc                               <  1:2922442/67‑1 (MQ=255)
                                   tcggTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCg                              >  1:3430540/4‑71 (MQ=255)
                                     ggTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGt                             <  1:4206116/69‑1 (MQ=255)
                                     ggTAAGGGTGGTTTCGCTATATCGTTCATGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGt                             <  1:4757730/69‑1 (MQ=255)
                                      gTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTg                           >  1:3768193/1‑71 (MQ=255)
                                       tAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTgg                          >  1:4654491/1‑71 (MQ=255)
                                         aGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGt                         >  1:3153443/1‑70 (MQ=255)
                                         aGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGt                         <  1:4929216/70‑1 (MQ=255)
                                         aGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGt                         >  1:3323326/1‑70 (MQ=255)
                                          gggTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTa                       <  1:2656801/71‑1 (MQ=255)
                                          gggTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTa                       <  1:3143180/71‑1 (MQ=255)
                                           ggtggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTAt                      <  1:2636503/71‑1 (MQ=255)
                                            gtggtTTCGCTATATCGTTCCTGGAAAAACAGTtgc                                                        >  1:465470/1‑36 (MQ=37)
                                            gtggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATc                     <  1:1384201/71‑1 (MQ=255)
                                             tggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGaa                                           >  1:2251778/1‑48 (MQ=38)
                                               gtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGa                   <  1:4946216/70‑1 (MQ=255)
                                               gtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAg                  <  1:821012/71‑1 (MQ=255)
                                                tttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCAt                                       <  1:2228083/49‑1 (MQ=38)
                                                tttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCg                    <  1:1258985/68‑1 (MQ=255)
                                                tttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCg                    <  1:4254962/68‑1 (MQ=255)
                                                tttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGt                 <  1:3203561/71‑1 (MQ=255)
                                                 ttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCt                                   <  1:2303866/52‑1 (MQ=39)
                                                 ttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGt                 >  1:3527110/1‑70 (MQ=255)
                                                  tCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGt                 >  1:142585/1‑69 (MQ=255)
                                                   cGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGt                 >  1:444656/1‑68 (MQ=255)
                                                    gCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGa             <  1:700905/71‑1 (MQ=255)
                                                    gCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGa             >  1:5084257/1‑71 (MQ=255)
                                                    gCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGa             >  1:3336446/1‑71 (MQ=255)
                                                      tatATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGAc            >  1:5202033/3‑70 (MQ=255)
                                                      tatATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGAc            >  1:3765017/3‑70 (MQ=255)
                                                       atATCGTTCCTGGAAAAACATTTGCTTCCATTGTCGAAc                                          <  1:1000342/38‑1 (MQ=25)
                                                       atATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                             <  1:5171122/35‑1 (MQ=37)
                                                       atATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAAc                                          <  1:1305214/38‑1 (MQ=38)
                                                       atATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGa          >  1:3577860/2‑71 (MQ=255)
                                                       atATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGa          >  1:5185783/2‑71 (MQ=255)
                                                       atATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGa          >  1:4633573/2‑71 (MQ=255)
                                                        tATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTa                <  1:2102027/64‑1 (MQ=255)
                                                        tATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACg           <  1:1812389/69‑1 (MQ=255)
                                                        tATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGAt         >  1:1228565/1‑71 (MQ=255)
                                                         aTCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGaa                                           >  1:797006/1‑36 (MQ=255)
                                                          tCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGt                             <  1:555889/49‑1 (MQ=255)
                                                          tCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGAttt       <  1:174857/71‑1 (MQ=255)
                                                          tCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGAttt       <  1:793488/71‑1 (MQ=255)
                                                          tCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGAttt       >  1:4221060/1‑71 (MQ=255)
                                                           cGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTg                           >  1:1476428/1‑50 (MQ=255)
                                                           cGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGAtttt      >  1:2875979/1‑71 (MQ=255)
                                                            gTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGAtttt      >  1:654575/1‑70 (MQ=255)
                                                            gTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTc     <  1:537189/71‑1 (MQ=255)
                                                            gTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTc     <  1:1314708/71‑1 (MQ=255)
                                                             ttCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGt                             <  1:5347754/46‑1 (MQ=255)
                                                             ttCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGt                             <  1:5341738/46‑1 (MQ=255)
                                                              tCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTaa               >  1:4526387/1‑59 (MQ=255)
                                                              tCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCaa   >  1:758331/1‑71 (MQ=255)
                                                              tCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCaa   >  1:1697322/1‑71 (MQ=255)
                                                              tCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCaa   >  1:1027207/1‑71 (MQ=255)
                                                               ccTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAAc  >  1:2581918/1‑71 (MQ=255)
                                                                cTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGa                   <  1:3760764/53‑1 (MQ=255)
                                                                cTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAAc  >  1:2211093/1‑70 (MQ=255)
                                                                   |                                                                  
CGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCAGTAAGGGTGGTTTCGCTGTATCGTTCCTGAAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAAC  >  AP009048/3684650‑3684783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: