| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 70,581 | C→T | 29.3% | V65V (GTC→GTT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 70,581 | 0 | C | T | 29.3% | 117.2 / 58.8 | 75 | V65V (GTC→GTT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (33/20); new base T (16/6); total (49/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCT > AP009048/70511‑70650 | tGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTc < 1:2563541/71‑1 (MQ=255) aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTg > 1:3880503/1‑71 (MQ=255) aaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGc > 1:366112/1‑71 (MQ=255) aGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGcc > 1:4250894/1‑71 (MQ=255) aGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGc > 1:4311839/1‑70 (MQ=255) ggTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGcc > 1:1278682/1‑70 (MQ=255) ggTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGcc > 1:2884538/1‑70 (MQ=255) ggTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCg < 1:529983/71‑1 (MQ=255) gTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGa < 1:953937/71‑1 (MQ=255) ttATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCt > 1:4065915/1‑65 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCg > 1:590614/1‑71 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGAcc < 1:3117049/70‑1 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGAc > 1:502602/1‑69 (MQ=255) tatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCg < 1:4476863/70‑1 (MQ=255) ctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:3745008/1‑71 (MQ=255) cAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGata > 1:4875706/1‑71 (MQ=255) aaTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat < 1:1048507/71‑1 (MQ=255) aTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGata > 1:397074/1‑69 (MQ=255) tctcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCg < 1:4562010/57‑1 (MQ=255) cACCATTCGCGGTCATGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:2191455/1‑71 (MQ=255) cACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGAcc < 1:4427440/57‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAttt < 1:4108131/67‑1 (MQ=255) cATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg > 1:2095143/1‑67 (MQ=255) aTTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCgg > 1:4954919/1‑55 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGa > 1:2254462/1‑50 (MQ=255) gcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCg > 1:3489366/1‑71 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGc > 1:262648/1‑70 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGc > 1:1615428/1‑70 (MQ=255) cAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:4865216/1‑70 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:424527/1‑69 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAgg < 1:3655747/70‑1 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:1983318/1‑69 (MQ=255) gggggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGt < 1:581177/46‑1 (MQ=255) ggggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:3630727/1‑47 (MQ=255) ggggTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGAc < 1:3990789/40‑1 (MQ=255) gggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCg > 1:2897657/1‑41 (MQ=255) gggTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg > 1:3231651/1‑53 (MQ=255) ggtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:3744594/1‑70 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca > 1:3186627/1‑71 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtc < 1:2573053/70‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:1857965/71‑1 (MQ=255) gAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat > 1:1117994/1‑70 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagaga > 1:1818623/1‑64 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:5074428/1‑71 (MQ=255) aaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:2481030/1‑71 (MQ=255) aaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca < 1:878414/70‑1 (MQ=255) aaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:3002723/1‑70 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGtt > 1:1217733/1‑48 (MQ=255) aaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:2922700/1‑70 (MQ=255) aTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga > 1:627644/1‑58 (MQ=255) aTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc > 1:4153671/1‑71 (MQ=255) cAGGGACGAGAATTTTTCTGCCGACCGGGTGATATTTTg > 1:1318595/1‑39 (MQ=255) cAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCt > 1:1972826/1‑41 (MQ=255) cAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACgg > 1:3749215/1‑71 (MQ=255) aGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTa > 1:5277844/1‑67 (MQ=255) gggACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtc < 1:3327281/71‑1 (MQ=255) cGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTAATCACTACGGTCGTCa > 1:1506489/1‑71 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcgtc > 1:3080912/1‑69 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagag < 1:544507/50‑1 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt > 1:3646145/1‑71 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt < 1:1591208/71‑1 (MQ=255) agaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACg < 1:1241039/62‑1 (MQ=255) agaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATc < 1:2886835/71‑1 (MQ=255) gaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtc > 1:2315395/1‑52 (MQ=255) gaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcgt > 1:4131214/1‑66 (MQ=255) aaTTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCg < 1:566476/38‑1 (MQ=255) aaTTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga < 1:140469/44‑1 (MQ=255) aaTTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtc > 1:2795038/1‑63 (MQ=255) tttGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt < 1:1697628/66‑1 (MQ=255) tttGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:5043854/1‑69 (MQ=255) ttGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagaga < 1:3589843/45‑1 (MQ=255) ttGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagaga < 1:2903514/45‑1 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcgt > 1:2428617/1‑61 (MQ=255) gTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAgg < 1:5012641/70‑1 (MQ=255) gTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGc > 1:9150/1‑71 (MQ=255) tCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCt < 1:2120143/71‑1 (MQ=255) | TGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCT > AP009048/70511‑70650 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |