| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 155,463 | A→T | 100% | *247K (TAA→AAA) | ecpD ← | predicted periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 155,463 | 0 | A | T | 100.0% | 234.9 / NA | 77 | *247K (TAA→AAA) | ecpD | predicted periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (40/37); total (40/37) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAA > AP009048/155395‑155530 | tgatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa < 1:3619228/71‑1 (MQ=255) gatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg > 1:2603509/1‑71 (MQ=255) atgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc > 1:2454417/1‑71 (MQ=255) tgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc < 1:1735630/71‑1 (MQ=255) tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg > 1:2713475/1‑71 (MQ=255) tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg > 1:4181576/1‑70 (MQ=255) tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTa > 1:4720342/1‑71 (MQ=255) ttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc > 1:3488313/1‑71 (MQ=255) ttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc > 1:2539098/1‑71 (MQ=255) gTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCt < 1:5148682/70‑1 (MQ=255) ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt > 1:3558132/1‑71 (MQ=255) ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt < 1:5274570/71‑1 (MQ=255) ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt < 1:5250861/71‑1 (MQ=255) tCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg > 1:2341397/1‑51 (MQ=255) cTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgc > 1:2684209/1‑71 (MQ=255) cTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgc > 1:2863302/1‑71 (MQ=255) tataGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCa > 1:2436367/1‑56 (MQ=255) gTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGcca > 1:2394295/1‑70 (MQ=255) tCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCt > 1:3775053/1‑47 (MQ=255) tCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt < 1:577340/53‑1 (MQ=255) aCGCTATGCTTCCTCCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca < 1:2156593/70‑1 (MQ=255) aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAg < 1:2858375/68‑1 (MQ=255) aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa > 1:644598/1‑71 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc < 1:1173016/38‑1 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCa < 1:3218119/52‑1 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt > 1:4287585/1‑71 (MQ=255) gCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa > 1:664251/1‑52 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg > 1:1007634/1‑38 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAcc < 1:3220990/46‑1 (MQ=255) ttCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgc < 1:445056/54‑1 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg > 1:3145197/1‑36 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc > 1:5133697/1‑42 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa < 1:3254353/69‑1 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATg > 1:222218/1‑71 (MQ=255) cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt < 1:2798870/39‑1 (MQ=255) gCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAt < 1:5170600/36‑1 (MQ=255) cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGcc > 1:3509645/1‑51 (MQ=255) cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa < 1:4702319/61‑1 (MQ=255) cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGt < 1:810039/71‑1 (MQ=255) ggAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca < 1:59977/54‑1 (MQ=255) ggAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCaaa > 1:1912152/1‑56 (MQ=255) ggAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCaaa > 1:5136727/1‑56 (MQ=255) gAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaa < 1:1773985/71‑1 (MQ=255) aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaa < 1:2399343/71‑1 (MQ=255) aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaa > 1:3514838/1‑70 (MQ=255) aTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa < 1:3143125/71‑1 (MQ=255) aTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaa > 1:1142217/1‑70 (MQ=255) ttATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcg < 1:2061954/44‑1 (MQ=255) ttATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaaa > 1:4741616/1‑71 (MQ=255) tataTCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaaa < 1:3689816/70‑1 (MQ=255) tataTCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaa < 1:1945107/68‑1 (MQ=255) tataTCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaa < 1:1951222/68‑1 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa > 1:225110/1‑37 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa > 1:325053/1‑37 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAAt < 1:4762007/70‑1 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATg > 1:4161112/1‑71 (MQ=255) tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCaaa > 1:3731518/1‑49 (MQ=255) tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa < 1:914243/57‑1 (MQ=255) tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATg < 1:4158664/70‑1 (MQ=255) atCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa < 1:821079/52‑1 (MQ=255) atCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa < 1:2279649/66‑1 (MQ=255) ccTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaaa < 1:3700940/65‑1 (MQ=255) cTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTa > 1:2781028/1‑52 (MQ=255) ttGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCaaa < 1:3178076/44‑1 (MQ=255) ttGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAg > 1:769309/1‑45 (MQ=255) ttGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAg > 1:3841114/1‑45 (MQ=255) tGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCAc > 1:2804666/1‑67 (MQ=255) gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGc < 1:4193189/54‑1 (MQ=255) gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg > 1:3753358/1‑71 (MQ=255) gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg > 1:1482727/1‑71 (MQ=255) gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg > 1:3156161/1‑71 (MQ=255) ccTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg < 1:416682/70‑1 (MQ=255) cTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGt < 1:232771/54‑1 (MQ=255) tGATTTCAGCCTGGTATTACCTTCAATTGCGCCACCAAAGTCATTAATgg < 1:2563018/50‑1 (MQ=255) tGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt < 1:1691217/45‑1 (MQ=255) gATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGt > 1:4412292/1‑52 (MQ=255) aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGaa > 1:5221089/1‑71 (MQ=255) | TGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAA > AP009048/155395‑155530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |