New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 = 389338469 (1.180)3 (0.060) 3/94 NT 4.7% coding (938/987 nt) yiaO predicted transporter
?AP009048 = 3893395 57 (1.060)coding (927/987 nt) yiaO predicted transporter
Rejected: Frequency below/above cutoff threshold.

CCCCAGCCAGTATTGCGTCATGGTTTATTGCACCTCATCCACGGCTTTCAGCAGATCAGCTCCCTGCGGCACAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/3893311‑3893384
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acatGTTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGAC  <  AP009048/3893395‑3893327
                                                                                                                                               
CCCCAGCCAGTATTGCGTCATGGTTTATTGCACCTCATCCACGGCTTTCAGCAGATCAGCTCCCTGCGGCA                                                                          >  1:224603/1‑71
CCCCAGCCAGTATTGCGTCATGGTTTATTGCACCTCATCCACGGCTTTCAGCAGATCAGATCCCTGCGGCA                                                                          >  1:2946320/1‑71
 CCCAGCCAGTATTGCGTCATGGTTTATTGCACCTCATCCACGGCTTTCAGAAGATCAGCTCCCTGCGGCAC                                                                         <  1:4417196/71‑1
   CAGCCAGTATTGCGTCATGGTTTATTGCACCTCATCCACGGCTTTCAGCAGATCAGCTCCCTGCGGCACAT                                                                       >  1:2465291/1‑71
   CAGCCAGTATTGCGTCATGGTTTATTGCACCTCATCCACGGCTTTCAGCAGATCAGCTCCCTGCGGCACAT                                                                       <  1:831723/71‑1
   CAGCCAGTATTGCGTCATGGTTTATTGCACCTCATCCACGGCTTTCAGCAGATCAGCTCCCTGCGGCA                                                                          <  1:2072032/68‑1
         GTATTGCGTCATGGTTTATTGCACCTCATCCACGGCTTTCAGCAGATCAGCTCCCTGCGGCACAT                                                                       <  1:2628929/65‑1
              GCGTCATGGTTTATTGCACCTCATCCACGGCTTTCAGCAGATCAGCTCCCTGCGGCAC                                                                         >  1:1410608/1‑58
                 TCATGGTTTATTGCACCTCATCCACGGCTTTCAGCAGATCAGCTCCCTGCGGCACATGTTTGTTAAAGATG                                                         <  1:5106415/71‑1
                         TATTGCACCTCATCCACGGCTTTCAGCAGATCAGCTCCCTGCGGCAC                                                                         >  1:78003/1‑47
                           TTGCACCTCATCCACGGCTTTCAGCAGATCAGCTCCCTGCGGCACAT                                                                       >  1:3471157/1‑47
                                   CATCCACGGCTTTCAGCAGATCAGCTCCCTGCGGCACATGTTTGTTAAAGATGTG                                                       >  1:2004282/1‑55
                                               TCAGCAGATCAGCTCCCTGCGGCACATGTTTGTTAAAGATGTG                                                       >  1:4945016/1‑43
                                                                      ACATGTTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGA                       >  1:2886970/1‑52
                                                                       CATGTTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGA   >  1:4207862/1‑71
                                                                        ATGTTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGAC  >  1:636466/1‑71
                                                                                                                                               
CCCCAGCCAGTATTGCGTCATGGTTTATTGCACCTCATCCACGGCTTTCAGCAGATCAGCTCCCTGCGGCACAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/3893311‑3893384
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acatGTTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGAC  <  AP009048/3893395‑3893327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.