Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC AP009048 654,214 Δ1,199 bp 100% [dcuC][dcuC] [dcuC], insH, [dcuC]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ AP009048 654214–655411 655412 2–1199 70 [0] [0] 72 [dcuC]–[dcuC] [dcuC],insH,[dcuC]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 = 6542130 (0.000)50 (0.950) 38/92 NT 100% coding (70/477 nt) dcuC anaerobic C4‑dicarboxylate transport
?AP009048 655413 = 0 (0.000)coding (978/1092 nt) dcuC anaerobic C4‑dicarboxylate transport

ATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/654140‑654213
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCG  >  AP009048/655413‑655481
                                                                                                                                               
ATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                          <  1:988701/71‑1
 TCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                          >  1:3363161/1‑70
  CAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                          >  1:2526576/1‑69
     GATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                          <  1:540136/66‑1
       TGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCC                                                                   >  1:803333/1‑71
         ATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTG                                                                 <  1:3406853/71‑1
         ATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCT                                                                  <  1:3083291/70‑1
         ATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCT                                                                  <  1:3465168/70‑1
         ATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCT                                                                  <  1:276666/70‑1
            CTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAG                                                               <  1:1820819/70‑1
             TCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGC                                                                           <  1:4051402/57‑1
              CGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                             >  1:3104844/1‑70
               GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAA                                                           >  1:322048/1‑71
               GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAA                                                           >  1:4351414/1‑71
               GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                             <  1:3034550/69‑1
               GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGC                                                              <  1:244159/68‑1
               GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGA                                                                >  1:434966/1‑66
                 CGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATA                                                         >  1:1804693/1‑71
                    ACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCC                                                      <  1:2402542/71‑1
                    ACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAA                                                                        <  1:4737729/53‑1
                      CAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCA                                                    <  1:1353199/71‑1
                      CAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCC                                                      >  1:2119903/1‑69
                      CAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAA                                                           <  1:511190/64‑1
                       AAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAG                                                   <  1:2023958/71‑1
                       AAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCC                                                                   >  1:742008/1‑55
                         ACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCT                                                                  >  1:140709/1‑54
                           GAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGC                                               <  1:2106577/71‑1
                           GAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGC                                               <  1:2435902/71‑1
                               TAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACC                                           <  1:1661059/71‑1
                                AGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                          <  1:4173026/39‑1
                                 GCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCA                                          <  1:203397/70‑1
                                 GCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGC                                               >  1:3035934/1‑65
                                 GCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                             <  1:2383816/51‑1
                                 GCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGC                                                              >  1:3534460/1‑50
                                    ATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAG                                      >  1:3850856/1‑71
                                    ATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAG                                      <  1:368971/71‑1
                                      AGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCA                                    >  1:1480840/1‑71
                                      AGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCA                                          >  1:3287061/1‑65
                                       GAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCAT                                   <  1:1759009/71‑1
                                       GAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCA                                    <  1:1640226/70‑1
                                         AATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCC                                                                   <  1:1959528/37‑1
                                           TCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGC                                        >  1:287550/1‑62
                                           TCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAAT                                                          <  1:1501608/44‑1
                                             AGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCAC                             <  1:1680240/71‑1
                                             AGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCA                                       <  1:4307777/61‑1
                                             AGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGA                                                            >  1:745839/1‑40
                                                CTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTT                          <  1:3835513/71‑1
                                                 TTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGA                                                            <  1:2913247/36‑1
                                                  TTGAATAAAGCCGATGGTGCTAAGCCCCTGATCGAATACCCCAGCGGCAACCAGCAGCATCACCAC                             <  1:113166/66‑1
                                                   TGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAG                        >  1:4942755/1‑70
                                                   TGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTA                         <  1:2677856/69‑1
                                                     AATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCA                      <  1:3059268/70‑1
                                                     AATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCA                      <  1:186699/70‑1
                                                      ATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAA                    >  1:2371598/1‑71
                                                        AAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCA                                    >  1:1106450/1‑53
                                                         AAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGC                 <  1:3383437/71‑1
                                                         AAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGC                 <  1:2530196/71‑1
                                                         AAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAAC                                            >  1:3114265/1‑44
                                                          AGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCA                >  1:3703534/1‑71
                                                           GCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCAT               >  1:2913195/1‑71
                                                            CCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCAC                             <  1:1340305/56‑1
                                                              GATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCA                      <  1:4968817/61‑1
                                                               ATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGC           <  1:5130038/71‑1
                                                               ATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCA                      <  1:3150541/60‑1
                                                                 GGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCA         >  1:5344110/1‑71
                                                                  GTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCAT        >  1:320036/1‑71
                                                                  GTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCA         <  1:4408574/70‑1
                                                                   TGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATC       >  1:2997590/1‑71
                                                                     CTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCAT               <  1:4566744/61‑1
                                                                     CTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCA                                    >  1:4938784/1‑40
                                                                       AAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC   >  1:876282/1‑71
                                                                        AGCCCCTGAGCGAATACCCCAGCGTCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC   <  1:3414168/70‑1
                                                                        AGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCG  <  1:2242070/71‑1
                                                                        AGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC   >  1:879877/1‑70
                                                                        AGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCAATCCCGC   >  1:710168/1‑70
                                                                        AGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGC                 <  1:2257030/56‑1
                                                                         GCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCG  >  1:1299901/1‑70
                                                                                                                                               
ATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/654140‑654213
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGCG  >  AP009048/655413‑655481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.