| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,736,260 | C→T | 5.5% | I468I (ATC→ATT) | fusA → | protein chain elongation factor EF‑G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,736,260 | 0 | C | T | 5.5% | 638.3 / 16.6 | 183 | I468I (ATC→ATT) | fusA | protein chain elongation factor EF‑G |
| Reads supporting (aligned to +/- strand): ref base C (80/93); new base T (4/6); total (84/99) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 194 total aligned reads displayed.
CGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGT > Exported/2736192‑2736329 | tATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGtt > 1:5814641/1‑71 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCTGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATg < 1:739670/71‑1 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAcatcat < 1:6404398/58‑1 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAcatcat < 1:4152947/58‑1 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt > 1:628054/1‑70 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt > 1:2326900/1‑70 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATg < 1:3796661/71‑1 (MQ=255) aagaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAg > 1:4082267/1‑71 (MQ=255) gaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCg > 1:5878672/1‑71 (MQ=255) aTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTg < 1:4693833/71‑1 (MQ=255) tAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGaa > 1:5299489/1‑70 (MQ=255) tAACCAGACCATCATCGCGGGTATGGGCGAACTGCACATCGACATCATCGTTGACCGTATGAAGCGTGa > 1:2949134/1‑69 (MQ=255) aCCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTc < 1:1333472/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:6506416/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:2469836/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:4463438/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:4937805/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:4957974/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:1388362/71‑1 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGAcatc > 1:6896862/1‑37 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGAcatc > 1:6436195/1‑37 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGAcatc > 1:3199533/1‑37 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGtt > 1:6341445/1‑70 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGtt > 1:6349278/1‑70 (MQ=255) tcatcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg > 1:6543640/1‑69 (MQ=255) atcGCGGGTATGGGCGAACTTCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:2174069/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:101987/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:4710064/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:2731120/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:5525506/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:426443/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:1823228/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:934541/1‑71 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt < 1:661245/53‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt < 1:6038362/53‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt < 1:4227830/53‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg < 1:177773/66‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg < 1:3731883/66‑1 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:172272/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:4982906/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:242732/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:5210485/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGa < 1:5370392/70‑1 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGa < 1:3426656/70‑1 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGa < 1:2824300/70‑1 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGa < 1:4530414/70‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:5248998/40‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:3682401/40‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:3051108/40‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:6473523/40‑1 (MQ=255) tATTGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:2272030/54‑1 (MQ=255) tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt < 1:1479535/39‑1 (MQ=16) tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt < 1:4345253/39‑1 (MQ=16) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGCATTCaa < 1:887262/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:6637131/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:564299/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:1560251/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAg > 1:3520655/1‑71 (MQ=255) aTGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:6780166/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:6208337/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:881510/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:4054594/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:311332/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:4283245/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:307313/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:1969056/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg > 1:6169055/1‑70 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:3339798/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg > 1:5954863/1‑70 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:5715923/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:5683570/70‑1 (MQ=255) tGGGCGAACTGCACCTCAACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg > 1:392734/1‑70 (MQ=255) gggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:2344979/69‑1 (MQ=255) ggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt > 1:795061/1‑53 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:5489715/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:5413812/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:4652862/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:3496298/1‑49 (MQ=255) cGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAc > 1:3319087/1‑71 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:4413281/1‑55 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:5134594/1‑55 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:1278170/1‑55 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc < 1:4104792/56‑1 (MQ=255) aaCTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCg > 1:811120/1‑71 (MQ=255) aCTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGc < 1:6628493/71‑1 (MQ=255) aCTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGc < 1:3722346/71‑1 (MQ=255) cTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCa < 1:5076167/71‑1 (MQ=255) tGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt < 1:4463348/46‑1 (MQ=255) tGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt < 1:1631101/46‑1 (MQ=255) gCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAgg > 1:4607324/1‑71 (MQ=255) gCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAgg > 1:3459250/1‑71 (MQ=255) cACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGt < 1:3285452/71‑1 (MQ=255) cACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGt < 1:2233858/71‑1 (MQ=255) cACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGt < 1:1101455/71‑1 (MQ=255) ccTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTg < 1:1888268/71‑1 (MQ=255) cGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTaaa > 1:2638174/1‑58 (MQ=255) gACATCATCGTTGACCGTATGAAGCGTGAATTCAACTTTGAAGCGAACGTAGGTAAACCGCAGGTTGCt < 1:4486772/69‑1 (MQ=255) gACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCt < 1:3580628/69‑1 (MQ=255) aCATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTAc < 1:6954121/71‑1 (MQ=255) catcatCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTAc > 1:6103541/1‑70 (MQ=255) | CGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGT > Exported/2736192‑2736329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |