Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,494,963 C→T 7.4% intergenic (+17/‑125) ibpB → / → yidF heat shock chaperone/predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,494,9630CT 7.4% 351.1 / 13.3 109intergenic (+17/‑125)ibpB/yidFheat shock chaperone/predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (56/44);  new base T (6/2);  total (63/46)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01

Only 100 of 113 total aligned reads displayed.

CATCGCAGCGCAGCGTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGTTTAA  >  Exported/2494895‑2495030
                                                                    |                                                                   
cATCGCAGCGCAGCGTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGccc                                                                   >  1:6475970/1‑71 (MQ=255)
      agcgcagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCAt                                                             <  1:759323/71‑1 (MQ=255)
      agcgcagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCAt                                                             <  1:51318/71‑1 (MQ=255)
      agcgcagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCAt                                                             <  1:5904388/71‑1 (MQ=255)
        cgcagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTc                                                           <  1:5721940/71‑1 (MQ=255)
         gcagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTc                                                           <  1:4276987/70‑1 (MQ=255)
         gcagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCt                                                          >  1:1827187/1‑71 (MQ=255)
          cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg                                                         >  1:2912738/1‑71 (MQ=255)
          cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg                                                         >  1:5349078/1‑71 (MQ=255)
          cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg                                                         <  1:1825029/71‑1 (MQ=255)
          cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg                                                         <  1:5400249/71‑1 (MQ=255)
          cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg                                                         <  1:1570800/71‑1 (MQ=255)
          cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg                                                         <  1:5848296/71‑1 (MQ=255)
          cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg                                                         <  1:6430225/71‑1 (MQ=255)
             cgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtggtg                                                      >  1:537489/1‑71 (MQ=255)
             cgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCCCCATTCtggtg                                                      >  1:3839563/1‑71 (MQ=255)
                 tCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCa                                                              >  1:6226721/1‑59 (MQ=255)
                 tCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCa                                                              >  1:4961241/1‑59 (MQ=255)
                 tCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCa                                                              >  1:4500740/1‑59 (MQ=255)
                  cGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGcc                                                               <  1:6221940/57‑1 (MQ=255)
                  cGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGcc                                                               <  1:6322925/57‑1 (MQ=255)
                  cGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGcc                                                               <  1:4212972/57‑1 (MQ=255)
                    cTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCttt                                               >  1:1634152/1‑71 (MQ=255)
                    cTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCttt                                               >  1:5449324/1‑71 (MQ=255)
                    cTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCttt                                               >  1:3099440/1‑71 (MQ=255)
                     tATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCtttt                                              >  1:4208789/1‑71 (MQ=255)
                       tCAGCGGACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGACATTCTGGTGGGGCtttttt                                            >  1:5221/1‑71 (MQ=255)
                            gAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGc                                                                     >  1:1813092/1‑41 (MQ=255)
                            gAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGc                                                                     >  1:1661176/1‑41 (MQ=255)
                            gAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGc                                                                     >  1:1379446/1‑41 (MQ=255)
                             aaCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTct                                       >  1:475217/1‑70 (MQ=255)
                             aaCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTct                                       >  1:4524106/1‑70 (MQ=255)
                             aaCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTct                                       >  1:4288952/1‑70 (MQ=255)
                             aaCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTct                                       >  1:2546769/1‑70 (MQ=255)
                             aaCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTct                                       >  1:4071682/1‑70 (MQ=255)
                              aCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg                                                         <  1:3296989/51‑1 (MQ=255)
                              aCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTctta                                     <  1:5924301/71‑1 (MQ=255)
                              aCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTctta                                     <  1:4017666/71‑1 (MQ=255)
                               cGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtggtg                                                      >  1:6660923/1‑53 (MQ=255)
                               cGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtggtg                                                      >  1:2292277/1‑53 (MQ=255)
                               cGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtggtg                                                      >  1:3513719/1‑53 (MQ=255)
                               cGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttac                                    >  1:2087339/1‑71 (MQ=255)
                                gTCCAGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttact                                   <  1:4902753/71‑1 (MQ=255)
                                  cccGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCtttt                                              <  1:5001250/58‑1 (MQ=255)
                                  cccGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCtttt                                              <  1:4171463/58‑1 (MQ=255)
                                     gcgTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTtacttact                               >  1:1689531/1‑70 (MQ=255)
                                     gcgTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTtacttact                               >  1:6037723/1‑70 (MQ=255)
                                     gcgTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTtacttact                               >  1:4607143/1‑70 (MQ=255)
                                     gcgTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTtacttact                               >  1:3153008/1‑70 (MQ=255)
                                      cgTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTca                             <  1:513111/71‑1 (MQ=255)
                                        ttAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCAt                                                             <  1:3834708/37‑1 (MQ=255)
                                        ttAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCAt                                                             <  1:1988624/37‑1 (MQ=255)
                                        ttAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCAt                                                             <  1:5787307/37‑1 (MQ=255)
                                          aaaTAGCTAACTAGCTATTCTCTTTGCCCCGCCATTc                                                           >  1:2366238/1‑37 (MQ=255)
                                           aaTAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTcaacaa                         <  1:1170927/70‑1 (MQ=255)
                                            aTAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATg                       <  1:4183975/71‑1 (MQ=255)
                                             tAGCTAACTAGCTATTCTCTTTGTCCCGCCATTCtg                                                         <  1:5778669/36‑1 (MQ=255)
                                             tAGCTAACTAGCTATTCTCTTTGTCCCGCCATTCtg                                                         <  1:6232719/36‑1 (MQ=255)
                                               gCTAACTAGCTATTCTCTTTGTCCCGCCATTCtggt                                                       >  1:5429251/1‑36 (MQ=255)
                                               gCTAACTAGCTATTCTCTTTGTCCCGCCATTCtggt                                                       >  1:6395765/1‑36 (MQ=255)
                                               gCTAACTAGCTATTCTCTTTGTCCCGCCATTCtggt                                                       >  1:505199/1‑36 (MQ=255)
                                               gCTAACTAGCTATTCTCTTTGTCCCGCCATTCtggt                                                       >  1:3310108/1‑36 (MQ=255)
                                               gCTAACTAGCTATTCTCTTTGTCCCGCCATTCtggt                                                       >  1:4904037/1‑36 (MQ=255)
                                                cTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttactta                                 >  1:5212998/1‑57 (MQ=255)
                                                cTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttactta                                 >  1:2829380/1‑57 (MQ=255)
                                                   aCTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttactta                                 <  1:1975227/54‑1 (MQ=255)
                                                   aCTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTaaa                >  1:3168883/1‑71 (MQ=255)
                                                   aCTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTaaa                >  1:1890366/1‑71 (MQ=255)
                                                     tAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttactt                                  >  1:6518084/1‑51 (MQ=255)
                                                     tAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttactt                                  >  1:2658257/1‑51 (MQ=255)
                                                     tAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTtacttact                               >  1:4121727/1‑54 (MQ=255)
                                                     tAGCTATGCTCTTTGCCCCGCCATTCTGGGGGGGCTTTTTTTGTctta                                     <  1:2442992/48‑1 (MQ=38)
                                                       gCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTc            <  1:2772245/71‑1 (MQ=255)
                                                       gCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTc            >  1:1154768/1‑71 (MQ=255)
                                                       gCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTc            <  1:1011267/71‑1 (MQ=255)
                                                        cTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTc            >  1:5618896/1‑70 (MQ=255)
                                                           ttCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTcaac                           <  1:6373689/52‑1 (MQ=255)
                                                           ttCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACt        >  1:4712718/1‑71 (MQ=255)
                                                           ttCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACt        >  1:1467589/1‑71 (MQ=255)
                                                            tctcTTTGCCCCGCCATTCTGGTGGGGCttttttt                                           <  1:3018298/35‑1 (MQ=255)
                                                             ctctTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTc                              >  1:1096929/1‑47 (MQ=255)
                                                             ctctTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGt      >  1:722976/1‑71 (MQ=255)
                                                             ctctTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGt      >  1:2683673/1‑71 (MQ=255)
                                                             ctctTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGt      >  1:138295/1‑71 (MQ=255)
                                                             ctctTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGt      >  1:2821715/1‑71 (MQ=255)
                                                             ctctTTGCCCCGCCATTCTGGTGGGGCGTTTTTTGTCTTACTTACTc                              >  1:4530945/1‑47 (MQ=255)
                                                              tctTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGtt     >  1:3467213/1‑71 (MQ=255)
                                                              tctTTGACCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGtt     >  1:11917/1‑71 (MQ=255)
                                                                tttGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTaa                 >  1:6473975/1‑57 (MQ=255)
                                                                tttGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGttt    >  1:3133536/1‑70 (MQ=255)
                                                                tttGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGttt    >  1:525764/1‑70 (MQ=255)
                                                                tttGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGt      <  1:3406764/68‑1 (MQ=255)
                                                                tttGCCACGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGtt     >  1:1854/1‑69 (MQ=255)
                                                                 ttGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTg       <  1:3019747/66‑1 (MQ=255)
                                                                  tGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttactta                                 >  1:1036428/1‑39 (MQ=255)
                                                                  tGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGttt    <  1:5958005/68‑1 (MQ=255)
                                                                  tGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGttt    <  1:5862515/68‑1 (MQ=255)
                                                                  tGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGtt     >  1:4051131/1‑67 (MQ=255)
                                                                  tGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGTTTaa  <  1:5950166/70‑1 (MQ=255)
                                                                  tGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGTTTaa  <  1:6574581/70‑1 (MQ=255)
                                                                    |                                                                   
CATCGCAGCGCAGCGTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGTTTAA  >  Exported/2494895‑2495030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: