BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,359,876	C→T	51.0%	noncoding (1219/2905 nt)	rrlA ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,359,876	0	C	T	51.0%	57.2 / inf	223	noncoding (1219/2905 nt)	rrlA	23S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base C (67/37); new base T (64/49); total (137/86)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 223 total aligned reads displayed.

CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTG  >  Exported/2359815‑2359945

 ccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac                                                             <  1:1343096/71‑1 (MQ=32)

 ccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac                                                             <  1:500881/71‑1 (MQ=32)

   gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg                                                           >  1:1244299/1‑71 (MQ=255)

   gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg                                                           >  1:1635997/1‑71 (MQ=255)

   gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg                                                           >  1:5535156/1‑71 (MQ=255)

      ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                                        >  1:4784833/1‑71 (MQ=34)

       tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                                        >  1:4268511/1‑70 (MQ=35)

       tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                                        <  1:2302443/70‑1 (MQ=35)

       tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTcaca                                                            >  1:3505045/1‑66 (MQ=11)

       tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTcaca                                                            >  1:455114/1‑66 (MQ=11)

       tATCGTTACTTAAGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTcaca                                                            >  1:6953648/1‑66 (MQ=11)

         tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATTCCTCACAGCACACCTTCACAGGCtt                                                       >  1:4087961/1‑69 (MQ=25)

         tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                                      <  1:6313178/70‑1 (MQ=35)

         tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTAc                                                     <  1:4186845/71‑1 (MQ=255)

         tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTAc                                                     <  1:6708360/71‑1 (MQ=255)

           gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                                      >  1:3779070/1‑68 (MQ=35)

           gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                                      >  1:3951941/1‑68 (MQ=35)

           gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                   <  1:2177686/71‑1 (MQ=34)

           gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                   <  1:5084278/71‑1 (MQ=34)

           gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACa                                                    <  1:6764703/70‑1 (MQ=255)

              aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAAc                                                >  1:5717927/1‑71 (MQ=34)

                   tGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                            <  1:3458748/70‑1 (MQ=255)

                     tCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc                                         <  1:5136959/71‑1 (MQ=255)

                      cAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc                                         >  1:1891344/1‑70 (MQ=255)

                       aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcc                                           <  1:6161038/67‑1 (MQ=11)

                       aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcc                                           <  1:5595732/67‑1 (MQ=11)

                       aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa                                       >  1:6449304/1‑71 (MQ=255)

                       aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa                                       >  1:6031892/1‑71 (MQ=255)

                        gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc                                      >  1:3039793/1‑71 (MQ=255)

                        gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc                                      >  1:4626457/1‑71 (MQ=255)

                         cATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                                     <  1:4987930/71‑1 (MQ=34)

                         cATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                              <  1:6701034/62‑1 (MQ=11)

                         cATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                              <  1:1835686/62‑1 (MQ=11)

                          aTTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                              <  1:4488663/61‑1 (MQ=11)

                          aTTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                                    <  1:4967176/71‑1 (MQ=255)

                           ttCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCca                                   <  1:967710/71‑1 (MQ=34)

                            tCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                                  >  1:6560609/1‑71 (MQ=255)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:6191674/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:520430/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:6628135/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:282853/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:3200033/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:3370982/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:6005640/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                                  >  1:2340363/1‑70 (MQ=255)

                             cGCACTTCTGATACCTCCAGAATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                                  >  1:5326964/1‑70 (MQ=255)

                               cACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                               <  1:4387181/71‑1 (MQ=34)

                                aCTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                            <  1:3317575/57‑1 (MQ=11)

                                 cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACa                                                    <  1:3547494/48‑1 (MQ=11)

                                 cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg                             <  1:2116592/71‑2 (MQ=255)

                                   tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAgg                                                          >  1:5428053/1‑40 (MQ=14)

                                   tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                               >  1:410865/1‑67 (MQ=255)

                                   tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                               >  1:248852/1‑67 (MQ=255)

                                   tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                               >  1:4869159/1‑67 (MQ=255)

                                    cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTATCCAACAAcaca                           >  1:5963505/1‑70 (MQ=255)

                                    cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                           <  1:3062870/70‑1 (MQ=40)

                                    cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                           <  1:3808528/70‑1 (MQ=40)

                                    cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                           >  1:5348448/1‑70 (MQ=40)

                                    cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                            >  1:6077081/1‑53 (MQ=11)

                                    cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                            >  1:472674/1‑53 (MQ=11)

                                    cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaac                              <  1:1987188/67‑1 (MQ=255)

                                     tGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                           >  1:2647114/1‑69 (MQ=40)

                                       aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                           >  1:1050833/1‑67 (MQ=40)

                                       aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                           >  1:844228/1‑67 (MQ=40)

                                       aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                           >  1:922961/1‑67 (MQ=40)

                                       aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAg                        >  1:6238992/1‑70 (MQ=255)

                                        tACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                                        <  1:3624654/37‑1 (MQ=14)

                                        tACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                                        <  1:5846793/37‑1 (MQ=14)

                                          ccTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTc                    >  1:4057187/1‑71 (MQ=255)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTa                                       >  1:1394192/1‑49 (MQ=38)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTa                                       >  1:436391/1‑49 (MQ=38)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAacac                            >  1:6365402/1‑60 (MQ=40)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAacac                            >  1:6855454/1‑60 (MQ=40)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAacac                            >  1:838629/1‑60 (MQ=40)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAacac                            >  1:1011665/1‑60 (MQ=40)

                                              cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCTACAACACATAGTGTCGCTg                >  1:1892633/1‑71 (MQ=255)

                                               aGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGc               >  1:5672033/1‑71 (MQ=255)

                                               aGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGc               >  1:166243/1‑71 (MQ=255)

                                                     ccTCACAGTACCCCTTCACAGGCTTACAGAACGCTCCCCTAcc                                     >  1:6339784/1‑43 (MQ=14)

                                                     ccTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                                     >  1:2275058/1‑43 (MQ=255)

                                                     ccTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                                     >  1:272999/1‑43 (MQ=255)

                                                     ccTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                                     >  1:5564895/1‑43 (MQ=255)

                                                     ccTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                                     >  1:5268238/1‑43 (MQ=255)

                                                     ccTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                                     >  1:5832654/1‑43 (MQ=255)

                                                     ccTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc                                      >  1:2391074/1‑42 (MQ=255)

                                                     ccTCACAGCACACCTTCACAGGCTTTCAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGc         <  1:317347/71‑1 (MQ=255)

                                                     ccTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGc         >  1:5652450/1‑71 (MQ=255)

                                                      cTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacgc                            >  1:5248167/1‑49 (MQ=255)

                                                      cTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacgc                            >  1:666506/1‑49 (MQ=255)

                                                      cTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacgc                            >  1:3511473/1‑49 (MQ=255)

                                                      cTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacgc                            >  1:2571655/1‑49 (MQ=255)

                                                      cTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCt        >  1:2944490/1‑71 (MQ=255)

                                                        cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCca                                   <  1:6573960/42‑1 (MQ=38)

                                                        cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCca                                   <  1:6690101/42‑1 (MQ=38)

                                                        cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCca                                   <  1:4247621/42‑1 (MQ=38)

                                                        cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCca                                   <  1:6961911/42‑1 (MQ=38)

                                                         acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCtt       <  1:5182970/69‑1 (MQ=255)

                                                         acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCtt       <  1:177367/69‑1 (MQ=255)

                                                         acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGGCGCTGCCGCAGCTTCg     <  1:2453938/71‑1 (MQ=255)

                                                            gCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTg  >  1:3746703/1‑71 (MQ=255)

CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTG  >  Exported/2359815‑2359945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`